ABSTRACT
Lachema kits ENTEROtest 24, ENTERO-Rapid 24, ENTERO-Screen, NEFERMtest 24, STAPHYtest 16, STREPTOtest 16, En-COCCUS test were used in identification of 871 strains of microorganisms isolated from clinical material. The kits proved to be highly reliable: they identified 88.8-97.5% cultures and permitted rapid (within 4 h) identification of enterobacteria most often occurring in clinical practice. The advantages of these kits are stripped plates allowing identification of cultures in individual stripes, a wide spectrum of biochemical tests, modern composition of biosubstrates in suspension media, and rational disposition of the tests, allowing technologically simpler identification of a wider spectrum of cultures in one stage without difficult accessory tests. Comparison of the above kits with kits manufactured by bioMérieux (France) showed a 84.6-100% correlation of the results.
Subject(s)
Bacterial Infections/diagnosis , Bacteriological Techniques/instrumentation , Bacteria/isolation & purification , Bacteria/metabolism , Bacterial Infections/microbiology , Bacteriological Techniques/statistics & numerical data , Czech Republic , Evaluation Studies as Topic , France , Humans , Indicators and ReagentsABSTRACT
Thirty-five specimens of pasteurized milk were tested for bacterial contamination by rapid autocalibration photometry for liquid, semiliquid, and homogenized hard foodstuffs, developed by the Ecotest Firm, Russia. Photometric (turbidimetric) analysis was carried out using the Labsystems (Finland) iEMS Reader plate photometer and Russian Analitika BACT software. The results of traditional analysis and the new rapid method developed by Ecotest were in good correlation, the differences did not surpass the magnitude of errors of measurements. The new method takes just 10-15 h instead of the routine 72 h and requires 300 times less nutrient media.
