[Lipid and protein peroxidation process and catalase activity in pre-eclamptic placenta]. / Procesy peroksydacji lipidów i bialek oraz aktywnosc katalazy w lozyskach z ciaz powiklanych stanem przedrzucawkowym.

There are some suggestions that free radicals are involved in some dysfunctions observed in preeclampsia. In this study we have examined the antioxidant status of preeclamptic placentas. We have used placentas obtained from normal pregnant women and women with preeclampsia. Lipoperoxidative process was measured by means of Okhawa method. Sedlak method was used to measured the total thiol groups. The catalase activity was measured by means of Pffeifer method. The results show that the catalase activity decreases, the amount of MDA increases and the total amount of thiol groups is smaller in preeclamptic placentas. The level of lipid peroxides in preeclamptic placentas is about 1.8 times higher in comparison with normal placentas. The decreased level of total thiol groups in preeclamptic placentas can be caused by a more intensive process of protein peroxidation. Catalase is less active in preeclamptic placentas. It can be due to lower activity of antioxidant systems or the destruction of antioxidant systems by reactive oxygen species. The results of our experiments confirm lower antioxidant status in preeclamptic placentas and suggest that peroxidative reaction may cause many dysfunctions associated with preeclampsia.

[The effect of Trolox-vitamin E analog on placental lipid peroxidation with physiologic pregnancies and pregnancies complicated by pre-eclampsia]. / Wplyw troloxu-analogu witaminy E na procesy peroksydacji lipidów w lozyskach kobiet z ciaz fizjologicznych oraz powiklanych stanem przedrzucawkowym.

There are suggestions that free radicals are involved in some dysfunctions observed in preeclampsia. In this study we have examined the effect of Trolox (water soluble vitamin E analog) on placental lipid peroxidation. Placentas from 20 normal and 20 preeclamptic woman were used in this study. Lipoperoxidative process was measured by means of Okhawa method. The level of lipid peroxides in preeclamptic placentas is higher than in normal placentas. In normal placentas 20 microM, of Trolox caused the greatest fall in MDA level (about 25%) in comparison with the control MDA level (only with peroxidative system without Trolox). In preeclamptic placentas the greatest fall in lipooxygenation process (about 14%) was caused by 25 microM. of Trolox. The influence of Trolox on the level of lipid peroxides in preeclamptic placentas is less effective in comparison with normal placentas.