In contrast to matrix metalloproteinases, serum adiponectin concentrations increase after radioiodine treatment of thyrotoxicosis.

BACKGROUND: Matrix metalloproteinases (MMPs), together with their tissue inhibitors (TIMPs), remodel extracellular matrix under physiological and pathological conditions and are implicated in pathogenesis of cardiovascular diseases, cancer and in chronic inflammation. We have endeavoured to assess whether concentrations of MMPs, TIMPs, and anti-inflammatory adiponectin are altered by pharmacological treatment of acute thyrotoxicosis or by radioiodine therapy (RIT). MATERIAL AND METHODS: We measured serum concentrations of MMP-2, MMP-9, TIMP-1, TIMP-2, and adiponectin, TSH, free T4 (FT4) and free T3 (FT3) in 15 patients (4 males), age (years) 51.8±15.3 (mean±SD) with hyperthyroidism treated with thiamazole (Group 1) and in 20 subjects (2 males), treated for thyrotoxicosis with radioiodine, age 52.3±12.4 (Group 2), where blood samples were taken before RIT, visit 1 (V1), seven days post RIT, visit 2 (V2), and two to three months post RIT, visit 3 (V3). RESULTS: In Group 1 there was no significant change in concentrations of MMP-2, MMP-9, TIMP-1, TIMP-2 or adiponectin, despite a fall in FT4 and FT3 (8.74±4.79 pg/ml vs 3.54±2.40 pg/ml, for FT3, and 4.48 ±2.21 ng/ml vs 1.02±1.07 ng/ml, for FT4, p<0.001). In Group 2 RIT did not cause any acute change in serum MMP-2, MMP-9, TIMP-1 and TIMP-2 or adiponectin (V1 vs V2). However, there was a significant increase in serum adiponectin [from 15201±8860 ng/ml (V1) to 19373±8657 ng/ml (at V3), p<0.05], and TIMP-2 at V3 [from 129±45 ng/ml (V1) to 149±38 ng/ml (V3), p<0.01]. There was no significant change MMP-2, MMP-9 and TIMP-1 between V1 and V3. There was a decrease in FT4 and FT3 from 24.4±15.4 pmol/l (V1) to 14.7±10.6 pmol/l (V3), and from 10.0±5.65 (V1) to 6.1±4.8 pmol/l (V2), p<0.01, for FT4 and FT3, respectively. CONCLUSIONS: Radioiodine therapy of thyrotoxicosis does not alter serum MMP-2, MMP-9 or TIMP-1 concentrations either acutely or after about three months of observation. An increase in serum adiponectin might reflect favourable effects of radioiodine administration on cardiovascular risk factors, while an increase in TIMP-2 (principal MMP-2 inhibitor) might lead to a decrease in free MMP-2 concentrations.

Effects of substance P on the growth processes in rat thyroid lobes in vitro.

The effects of substance P (SP) on (1) 3H-thymidine incorporation into the DNA of rat thyroid lobes during a 4-hour incubation, and (2) thymidine kinase (TK, EC 2.7.1.21) activity in homogenates of rat thyroid lobes were investigated. We also examined the influence of the joint action of SP and thyrotropin (TSH) on these processes. It was shown that the effects of SP on these processes were dependent on the concentration of the neuropeptide applied. At the lowest concentration (10(-12) M), SP significantly (p < 0.0025) decreased and at the highest one (10(-6) M) it slightly increased (p < 0.09) the values of the examined growth indices. The activity of TK rose in parallel with the increase in SP concentration. SP and TSH, when used together, exerted similar decreasing effects, both on 3H-thymidine incorporation into DNA and on TK activity.

Changes of 3H-thymidine incorporation into DNA and thymidine kinase activity in rat thyroid lobes, following their exposure to neuropeptide Y.

The effects of neuropeptide Y (NPY) on 3H-thymidine incorporation into DNA of rat thyroid lobes in a 4 h incubation, and on the activity of thymidine kinase (TK) in the homogenates of thyroid lobes in vitro, as well as the influence of thyrotropin (TSH), applied alone or together with NPY, were investigated. The effects of NPY were dependent on the concentration used. Neuropeptide Y, in the lowest and the highest concentrations, decreased both the incorporation of 3H-thymidine to DNA and TK activity, and when used in intermediate concentrations NPY did not significantly affect the processes examined. Thyrotropin decreased TK activity and revealed a tendency towards lowering 3H-thymidine uptake, the latter effect being not statistically significant. NPY (10(-10) M) used together with TSH, produced an increase of 3H-thymidine incorporation into DNA, when compared with controls and the TSH-exposed group. Following the joint exposure to NPY and TSH, TK activity declined.

Occurrence of goitre in relation to iodine deficiency in school children in Lódz City, the Lódz metropolitan area and in Piotrków, Sieradz, Plock, Wloclawek, Radom and Skierniewice Voivodships (Lódz coordinating center).

This paper presents the results of studies performed within the Applied Project of the Ministry of Health and Social Welfare and of the Committee for Scientific Research: "Studies on Iodine Deficiency in Poland", including the school children in Lódz City and seven voivodships of the Central Poland. According to the results, the analyzed regions can be regarded as areas of goitrous endemia of a moderate degree (the goitre was found in 32.7% of examined population); the same regions can also be classified among the regions of mild iodine deficiency (the mean iodide concentration in urine was 64.0 micrograms/l). The obtained results prove the to-date's iodine prophylaxis to be not entirely efficient in the areas of interest, demanding an effective model of iodine complementation in diet to be designed.

[Effects of prophylactic doses of potassium iodide on the course of thyroid diseases (1986-1990) diagnosed due to the atomic accident at Czernobyl in adult patients at the outpatient endocrinologic hospital clinic in Lodz]. / Wplyw profilaktycznej dawki jodku potasu na przebieg chorób tarczycy (1986-1990) zdiagnozowanych przed awaria elektrowni atomowej w Czarnobylu u doroslych pacjentów Poradni Endokrynologicznej Szpitala Klinicznego nr 3 Akademii Medycznej w Lodzi.

2521 patients of the Lódz Outpatient Endocrinological Clinic (2290 females, 231 males; inhabitants of the central region of Poland Lódz City, Lódz Metropolitan Area, Piotrków, Plock, Sieradz, Skierniewice and Wloclawek Provinces in which committed dose equivalent to the thyroid was between 2.7-7.0 mSv [min.-max.] in Skierniewice Province and 4.6-11.7 mSv in Plock Province) were included in the study. The patients were divided into 5 groups: I--persons who did not take the protective dose of potassium iodide (KI) after Chernobyl Nuclear Power Plant accident and did not received any treatment with thyroid preparations or hormones at that time (n = 1282), II--patients who receive KI, once or several times (n = 774), III--patients who took orally iodine tincture or other iodine-containing preparations for the above purposes (n-37), IV--patients who took tablets of Thyroideum (Polfa) Thyroideum siccum (dry thyroid extract), once or several times, as a prophylactic action (n = 79), V--patients who were in the course of continuous treatment with Thyreoideum or thyroid hormones at the time of Chernobyl accident (n = 349). The analysis was performed for all the patients jointly, as well as separately for: either sex, three age groups (18-30, 31-55, 56-70 yrs) and 7 administrative areas specified above. All the patients were subjected into complex clinical examination, serum TSH, T3, T4 concentrations, anti-thyroid membrane antibodies (ATMA) and antithyroglobulin antibodies (ATg) titres, as well as ultrasound, scintigraphy, and fine needle aspiration biopsy of the thyroid (the last two according to indications) included. The patients were also examined by means of a special questionnaire (Patient's Inquiry Sheet), which was subsequently submitted to computer analysis. All the doctors' diagnoses from 1986 (17 different diagnoses) and 1990 (27 different diagnoses), as well as the course of diseases, were verified with use of a specially prepared IBM PC/AT computer program ChernStat.(ABSTRACT TRUNCATED AT 400 WORDS)