ABSTRACT
A procedure for large-scale separation and purification of two mink serum macroglobulins, Lpm and alpha 2M, is described. Individual preparations of each of these macroglobulins were obtained in an immunologically pure state. After precipitation from the serum at 6.5-13% PEG 6000, Lpm and alpha 2M were separated by a pH stepwise gradient elution metal chelate affinity chromatography and purified by chromatographies on Biogel A 1.5 m and DEAE-Trisacryl M. Each of these macroglobulins was tested by counter-immunoelectrophoresis with the corresponding monospecific antiserum. The yields per 100 ml of the source serum were 23-44 mg of Lpm and 7-30 mg of alpha 2M which corresponded to 10-20% of their serum contents. Some of general biochemical properties of mink Lpm and alpha 2M and of all mammalian alpha-macroglobulins are discussed.
Subject(s)
Lipoproteins, HDL/isolation & purification , Mink/blood , alpha-Macroglobulins/isolation & purification , Animals , Chromatography, Liquid , Electrophoresis, Polyacrylamide Gel , Hydrogen-Ion Concentration , Lipoproteins, HDL/metabolism , alpha-Macroglobulins/metabolismABSTRACT
Distribution in the organs and tissues of two proteins of alpha-macroglobulin fraction, that differ in their antigenic structures, has been studied in the American mink. The both proteins (alpha 2M and Lpm) are present in hepatocytes, in cells of the follicular epithelium of the ovary, in the thymic bodies, in the alveolar macrophages of the lungs and in the splenic lymphoid nodes. Joint localization of alpha 2M and Lpm is revealed in the connective tissue of all the organs examined. The exception make the stomach and the uterine, where alpha 2M is revealed but not Lpm. The results obtained demonstrate a similar distribution of the two alpha-macroglobulins in the mink organism. They correspond to the literature data on morphofunctional topography of alpha 2M in the man. Certain individual differences in alpha 2M and Lpm localization can reflect peculiarities inherent in each of these alpha-macroglobulins of the American mink.
Subject(s)
Lipoproteins, HDL/metabolism , Mink/metabolism , alpha-Macroglobulins/metabolism , Animals , Female , Immunohistochemistry , Immunologic Techniques , Lipoproteins, HDL/analysis , Tissue Distribution , alpha-Macroglobulins/analysisSubject(s)
Contrast Media/pharmacology , Diatrizoate Meglumine/pharmacology , Diatrizoate/analogs & derivatives , Iodine/administration & dosage , Iodobenzoates/administration & dosage , Kidney Diseases/diagnostic imaging , Triiodobenzoic Acids/administration & dosage , Urography/methods , Adolescent , Child , Child, Preschool , Diuresis/drug effects , Drug Combinations , Humans , Kidney/physiopathologySubject(s)
Nephritis, Hereditary/diagnostic imaging , Adolescent , Adult , Age Factors , Child , Child, Preschool , Female , Humans , Infant , Male , Nephritis/diagnostic imaging , RadiographySubject(s)
Nephritis, Hereditary/diagnosis , Nephritis/diagnosis , Child , Diagnosis, Differential , Humans , MathematicsABSTRACT
In 75 children with hereditary nephritis, in 27 ones with hematuric form of acquired nephritis and in 55 parents dysplastic symptoms were investigated. A high differential and diagnostic value of disembriogenetic stigmata for hereditary nephritis is demonstrated. It is considered that connective tissue stigmata are one of the symptoms of hereditary nephritis. Some genetic and ontogenetic aspects of displastic symptoms formation in this case are discussed.
