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1.
J Neurosci ; 33(6): 2338-55, 2013 Feb 06.
Article in English | MEDLINE | ID: mdl-23392664

ABSTRACT

Androgens have dramatic effects on neuronal structure and function in hippocampus. However, androgen depletion does not always lead to hippocampal impairment. To address this apparent paradox, we evaluated the hippocampus of adult male rats after gonadectomy (Gdx) or sham surgery. Surprisingly, Gdx rats showed increased synaptic transmission and long-term potentiation of the mossy fiber (MF) pathway. Gdx rats also exhibited increased excitability and MF sprouting. We then addressed the possible underlying mechanisms and found that Gdx induced a long-lasting upregulation of MF BDNF immunoreactivity. Antagonism of Trk receptors, which bind neurotrophins, such as BDNF, reversed the increase in MF transmission, excitability, and long-term potentiation in Gdx rats, but there were no effects of Trk antagonism in sham controls. To determine which androgens were responsible, the effects of testosterone metabolites DHT and 5α-androstane-3α,17ß-diol were examined. Exposure of slices to 50 nm DHT decreased the effects of Gdx on MF transmission, but 50 nm 5α-androstane-3α,17ß-diol had no effect. Remarkably, there was no effect of DHT in control males. The data suggest that a Trk- and androgen receptor-sensitive form of MF transmission and synaptic plasticity emerges after Gdx. We suggest that androgens may normally be important in area CA3 to prevent hyperexcitability and aberrant axon outgrowth but limit MF synaptic transmission and some forms of plasticity. The results also suggest a potential explanation for the maintenance of hippocampal-dependent cognitive function after androgen depletion: a reduction in androgens may lead to compensatory upregulation of MF transmission and plasticity.


Subject(s)
CA3 Region, Hippocampal/physiology , Long-Term Potentiation/physiology , Mossy Fibers, Hippocampal/physiology , Synaptic Transmission/physiology , Testosterone/deficiency , Age Factors , Animals , Male , Neural Pathways/physiology , Orchiectomy , Rats , Rats, Inbred F344 , Testosterone/metabolism
2.
J Neurosci ; 31(17): 6392-7, 2011 Apr 27.
Article in English | MEDLINE | ID: mdl-21525279

ABSTRACT

Aquaporin-4 (AQP4) is the major water channel in the CNS and is primarily expressed in astrocytes. Little is known about the potential for AQP4 to influence synaptic plasticity, although many studies have shown that it regulates the response of the CNS to injury. Therefore, we evaluated long-term potentiation (LTP) and long-term depression (LTD) in AQP4 knock-out (KO) and wild-type mice. KO mice exhibited a selective defect in LTP and LTD without a change in basal transmission or short-term plasticity. Interestingly, the impairment in LTP in KO mice was specific for the type of LTP that depends on the neurotrophin BDNF, which is induced by stimulation at theta rhythm [theta-burst stimulation (TBS)-LTP], but there was no impairment in a form of LTP that is BDNF independent, induced by high-frequency stimulation. LTD was also impaired in KO mice, which was rescued by a scavenger of BDNF or blockade of Trk receptors. TrkB receptors, which mediate effects of BDNF on TBS-LTP, were not altered in KO mice, but p75NTR, the receptor that binds all neurotrophins and has been implicated in some types of LTD, was decreased. The KO mice also exhibited a cognitive defect, which suggests a new role for AQP4 and astrocytes in normal cognitive function. This defect was evident using a test for location-specific object memory but not Morris water maze or contextual fear conditioning. The results suggest that AQP4 channels in astrocytes play an unanticipated role in neurotrophin-dependent plasticity and influence behavior.


Subject(s)
Aquaporin 4/deficiency , Brain-Derived Neurotrophic Factor/metabolism , Memory Disorders , Neuroglia/metabolism , Neuronal Plasticity/physiology , Action Potentials/drug effects , Action Potentials/genetics , Action Potentials/physiology , Animals , Biophysics/methods , Carbazoles/pharmacology , Chi-Square Distribution , Disease Models, Animal , Electric Stimulation/methods , Enzyme Inhibitors/pharmacology , Immunoprecipitation , In Vitro Techniques , Indole Alkaloids/pharmacology , Long-Term Synaptic Depression/genetics , Maze Learning/drug effects , Maze Learning/physiology , Memory Disorders/genetics , Memory Disorders/pathology , Memory Disorders/physiopathology , Mice , Mice, Knockout , Neuronal Plasticity/drug effects , Neuronal Plasticity/genetics , Patch-Clamp Techniques , Receptor, trkB/metabolism , Signal Transduction/drug effects , Signal Transduction/genetics
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