[The Neisseria gonorhoeae and Chlamydia trachomatis coinfection in patients of Department of Dermatology and Venereology Medical University of Warsaw]. / Wsólistnienie zakazen Neisseria gonorhoeae i Chlamydia trachomatis u pacjentów zglaszajacych sie do Kliniki Dermatologii i Wenerologii Warszawskiego Uniwersytetu Medycznego.

INTRODUCTION: The aim of the study was to investigate the coexistence of N. gonorrhoeae and C. trachomatis infections in patients of Department of Dermatology and Venereology in Warsaw. METHODS: We investigated the urethral, cervical, anal and pharyngeal speci mens from 140 symptomatic and asymptomatic patients of Department of Dermatology and Venereology in Warsaw using the Real-Time PCR method. Real Time PCR DUPLICα® RealTime Neisseria gonorrhoeae and DUPLICα® RealTime Chlamydia trachomatis 2nd Generation Detection Kits (Euroclone®)was performed on termocycler Smart Cycler® Dx. For DNA isolation the Bact Extra Pure Kit (Euroclone®) was used. RESULTS: 22 samples were positive for C. trachomatis and 28 were positive for N. gonorrhoeae. Both infections coexisted in nine patients (6.4%). In our investigations, in opposition to results from other centers, gonorrhoea was more prevalent than C. trachomatis infection. The chlamydial infection coexisted in 32.1% with gonorrhea and gonorrhea coexisted with chlamydial infection in 40.9%. CONCLUSIONS: The coinfection with Neisseria gonorrhoeae and Chlamydia trachomatis occurs very often. According IUSTI and CDC recommendations patients with one kind of sexually transmitted disease (STI) diagnosed should be tested for the others. It is especially true in the case of gonorrhea and Chlamydia trachomatis infection. In IUSTI and CDC recommendations treatment for Chlamydia trachomatis is indicated in patients with gonorrhea.

[The Comparison of Real-Time PCR and direct immunofluorescence in laboratory diagnostics of chlamydiosis in patients of Department of Dermatology and Venereology Medical University of Warsaw]. / Porównanie metody Real-Time PCR z metoda bezposredniej immunofluorescencji w laboratoryjnej diagnostyce chlamydiozy u pacjentów Kliniki Dermatologii i Wenerologii Warszawskiego Uniwersytetu Medycznego.

INTRODUCTION: Chlamydial infection is one of the most common bacterial sexually transmitted diseases. The aim of the study was to compare Real Time PCR and Direct Immunofluorescence (DIF) in laboratory diagnostics of Chlamydia trachomatis infection in patients of Department of Dermatology and Venereology in Warsaw. METHODS: We investigated the urethral, cervical, anal and pharyngeal specimens from 152 patients of Department of Dermatology and Venereology in Warsaw. We used DNA Bact Extra Pure Kit (Euroclone®) to isolate DNA. Real Time PCR DUPLICα® 2nd Generation Detection Real Time Advanced Dual Easy Chlamydia trachomatis Kit (Euroclone®) was performed on termocycler Smart Cycler® Dx. For direct immunofluorescence we used MicroTrack Chlamydia trachomatis Direct Specimen Test (Trinity Biotech). RESULTS: In 90% of cases of Real Time PCR and DIF were consistent. 9% of samples were DIF negative and PCR positive and 1% were PCR negative and DIF positive. The results of PCR and DIF were identical more often in woman (98%)than in men (84%). In patients after antibiotics treatment the results of the two tests were consistent in 88%. CONCLUSIONS: In laboratory diagnostics of C. trachomatis infections the results of the Real Time PCR and DIF were highly consistent although Real Time PCR. was more sensitive than DIF.