[Antibiotic resistance and biofilm formation in Pseudomonas aeruginosa strains isolated from patients with urinary tract infections]. / Pseudomonas aeruginosa, její rezistence k vybraným antibiotikum a tvorba biofilmu u kmenu izolovaných od pacientu s infekcí mocových cest.

Altogether 118 Pseudomonas aeruginosa strains isolated from urine of patients with urinary tract infection were tested by the disk diffusion method for susceptibility to ciprofloxacin, ofloxacin, gentamicin, amikacin, colistin, meropenem, imipenem, piperacillin/tazobactam and ceftazidime. All strains were also screened for biofilm formation using a modified Christensen method. Eighty-eight, i.e., 74.6%, of the tested strains were resistant to ofloxacin, 86 (72.9%) to ciprofloxacin and 70 (59.3%) to gentamicin. Forty strains (33.9%) were resistant to imipenem, 42 strains (35.6%) to meropenem, 14 strains (11.9%) to amikacin, 2 strains (1.7%) to colistin, 35 strains (29.7%) to piperacillin/tazobactam and 41 strains (34.7%) to ceftazidime. Co-resistance to ofloxacin, ciprofloxacin and gentamicin was detected in 67 strains (56.8%) while 12 strains (10.2%) were resistant to most tested antibiotics, with the exception of amikacin and colistin. Biofilm formation was found in 41 strains (34.7%), more precisely in 23 of 46 inpatient strains and 18 of 72 outpatient strains. Eight (66.6%) of 12 polyresistant strains were biofilm producers.

[Squamous intraepithelial lesions in young female university students]. / Skvamózní intraepitelová léze u mladých zen, studujících vysokých skol.

The authors investigated the frequency of squamous intraepithelial lesions (SIL) in young women, in particular university students with special attention to morphological signs of the presence of human papilloma virus (HPV) in SIL low grade-SIL, LG-HPV. As compared with data in the world literature, they revealed a significantly lower rate of SIL LG, HPV-2.81% as compared with 5.5% reported in WHO studies of young women. The authors analyzed the biological behaviour of SIL, LG-HPV. As compared with data in the literature on known values of spontaneous regression which are 50-62% in the course of three years, they demonstrated during a time interval from 3 months to 2 years spontaneous regression in 39.1%. Further possibilities of regression in a longer time interval are expected in the group of clinically latent cytologically persistent LG lesions (18.1%) and in the group of patients which after termination of their studies were lost from the records (24.3%). During the investigated time interval no case of progression to a higher grade lesion was recorded. The authors reflect on diagnostic and therapeutic procedures with regard to the application of results of HPV typing.