ABSTRACT
Morphological, morphometric, and ultrastructural analysis of the nerve fibers in the colon mucosa was performed in C57BL/6 mice at various terms of development of acute colitis induced by dextran sodium sulphate. The nerve fibers were labeled with antibodies to pan-neuronal marker ßIII-tubulin. The progression of inflammatory and ulcerative processes in the mucosa on days 3-5 was associated with hyperplasia and hypertrophy of nerve fibers that peaked on day 7 after colitis induction. Ultrastructural analysis at all terms of colitis development showed moderate degeneration of axons. Thus, hypertrophy and hyperplasia of the nervous fibers in colon mucosa in experimental acute colitis correlated with aggravation of the ulcerative process in the mucosa. These changes are determined by alteration of histoarchitectonics and regenerative processes in the mucosa.
Subject(s)
Colitis/pathology , Colon/innervation , Intestinal Mucosa/innervation , Nerve Fibers/pathology , Acute Disease , Animals , Colitis, Ulcerative/pathology , Colon/pathology , Disease Models, Animal , Intestinal Mucosa/pathology , Male , Mice , Mice, Inbred C57BL , Microscopy, Electron , Nerve Fibers/ultrastructureABSTRACT
We studied the antiinflammatory effect of a Russian-made glucocorticoid budesonide administered by inhalation and intraperitoneal injection to rats with experimental noninfectious pulmonary granulomatosis. The antiinflammatory effects of budesonide at both administration routes were similar. Intraperitoneal injection, in contrast to inhalation, produced a systemic effect manifested in suppression of hyperplasia of non-encapsulated lymphoid formations in the mucosa.
Subject(s)
Anti-Inflammatory Agents/therapeutic use , Budesonide/therapeutic use , Lymphomatoid Granulomatosis/drug therapy , Administration, Inhalation , Animals , Bronchoalveolar Lavage Fluid , Hyperplasia/drug therapy , Infusions, Parenteral , Lung/drug effects , Male , Rats , Rats, Wistar , Time FactorsABSTRACT
Rats were exposed to a single (30 min) or repeated (30 min x 10, daily) normobaric hypoxia by a gas mixture with 10% of O2. In both cases urgent adaptive reactions develop in the lungs in the form of epithelium desquamation, increasing migration of neutrophils into the lung tissue, enhancement of labrocyte degranulation. Long-term adaptive reactions develop after the repeated exposures: proliferation of connective tissue labrocytes, hyperplasia of the lymphoid tissue associated with bronchi.
