ABSTRACT
The cancer stem cell (CSC) theory implicates a small subpopulation of cells with stem-like properties, which is responsible for tumour initiation, development and metastasis. The unique biological and functional characteristics of CSCs, widely associated with treatment resistance, indicate an association between metastasis and stemness. It was hypothesised that metastatic cell lines may be enriched in CSCs and that this would correlate with a more resistant tumour. In the present study, the SW480 and SW620 paired cell lines derived from a colon adenocarcinoma and its lymph node metastasis, respectively were compared as an in vitro model of cancer progression. Their chemosensitivity and CSC properties were investigated. A range of in vitro assays were performed, including the side population assay, ALDEFLUOR assay, tumoursphere assay and assessment of CSC-associated surface phenotypes. It was determined that the SW480 and SW620 cells exhibited similar growth rates, although the SW480 cells were more migratory in wound healing assays on collagen and fibronectin matrices. SW480 and SW620 cells displayed similar CSC profiles, however, SW480 cells demosntrated significantly greater tumoursphere forming efficiency over SW620 cells. Tumourspheres derived from SW480 and SW620 cells also displayed differential sensitivity to 5-fluorouracil, oxaliplatin, geldanamycin and novobiocin that was not apparent when cells were grown under adherent conditions. Taken together, these results suggest that although the two cell lines have similar levels of putative CSC populations, there are differences in their biology that cannot be explained by these CSC levels. To the best of our knowledge, this is the first study to conduct a detailed analysis of the CSC populations using multiple in vitro assays in a paired cell line model. These results have clinical relevance for the understanding of the differences between primary tumours and their metastatic counterparts.
ABSTRACT
UNLABELLED: The lay press, scientists, and physicians appear to believe that gymnasts are continually getting smaller and that their "smallness" is a health risk. PURPOSE: To assess the historical changes in the size and age of the US women's Olympic gymnastics teams from 1956 to 2008. METHODS: The official records from the US Olympic Committee and USA Gymnastics of Olympic team members were assessed at 2 levels: individual height, mass, age, and body-mass index (BMI) and the team performance scores and rankings. Fourteen Olympic teams with a total of 106 team members, including the alternates, were included. Trend analyses were conducted using linear and polynomial models. RESULTS: Simple linear correlations indicated that since 1956, height, mass, age, BMI, and team Olympic rank have been declining. However, second-order polynomial curve fits indicated that in the last 4 Olympic Games the members of the US women's gymnastics teams have been getting larger. CONCLUSION: Women Olympic gymnasts were getting smaller through approximately the 1980s and early 1990s. Since then the size of these gymnasts has increased. The minimum-age rule modifications may have played a role in athlete size changes along with a shift from the near dominance of the former communist Eastern Bloc.
