Silicon (Si) mitigates the negative effects of iron deficiency in common bean (Phaseolus vulgaris L.) by improving photosystem activities and nutritional status.

Silicon (Si) is the second most abundant element in the Earth's crust after oxygen. Its beneficial impact on crop development and yield, particularly under stressful conditions such as iron (Fe) deficiency, has been well documented. Fe deficiency is a critical constraint that limits crop production globally. The objective of this study was to investigate the effects of silicon (Na2SiO3) on common bean (Phaseolus vulgaris L. 'Coco Rose' variety) under iron-deficient conditions. The common bean plants were subjected to six treatments, which included three sufficient iron treatments (50 µM Fe) each paired with three varying silicon concentrations (0, 0.25, and 0.5 mM Si), and three iron-deficient treatments (0.1 µM Fe) each associated with the same silicon concentrations (0, 0.25, and 0.5 mM Si). The results indicate that iron deficiency had a negative impact on almost all the measured parameters. However, under silicon treatments, especially with 0.5 mM Si, the depressive effects of iron deficiency were significantly mitigated. The addition of 0.5 mM Si alleviated leaf chlorosis and improved biomass production, nutritional status, photosynthetic pigment content, photosynthetic gas exchange, and photosystem (PSI and PSII) activities. Interestingly, a greater beneficial effect of silicon was observed on PSII compared to PSI. This was accompanied by a significant augmentation in leaf iron concentration by 42%. Therefore, by enhancing the photosystem activities and nutritional status, among other mechanisms, silicon is capable of mitigating the adverse effects of iron-deficient conditions, making it a successful and effective solution to cope with this nutritional stress.

Changes in organic compounds secreted by roots in two Poaceae species (Hordeum vulgare and Polypogon monspenliensis) subjected to iron deficiency.

Despite their economic and ecological interests, Poaceae are affected by the low availability of iron in calcareous soils. Several studies focused on the capacity of this family to secrete phytosiderophores and organic acids as a mechanism of tolerance to iron deficiency. This work aimed at studying the physiological responses of two Poaceae species; Hordeum vulgare (cultivated barley) and Polypogon monspenliensis (spontaneous species) to iron deficiency, and evaluate especially the release of phytosiderophores and organic acids. For this purpose, seedlings of these two species were cultivated in complete nutrient solution with or without iron. The biomass production, iron status, phytosiderophores and organic acids release by roots were studied. The results demonstrated that Polypogon monspenliensis was relatively more tolerant to iron deficiency than Hordeum vulgare. Polypogon monspenliensis had the ability to secrete a higher amount of phytosiderophores and organic acids, especially citric, acetic, oxalic and malic acids, compared to Hordeum vulgare. We propose this spontaneous species as a forage plant in calcareous soils and in intercropping systems with fruit trees to prevent iron chlorosis.

Natural Variation in Physiological Responses of Tunisian Hedysarum carnosum Under Iron Deficiency.

Iron (Fe) is an essential element for plant growth and development. The cultivation of leguminous plants has generated strong interest because of their growth even on poor soils. Calcareous and saline soils with poor mineral availability are wide-spread in Tunisia. In an attempt to select better forage crops adapted to Tunisian soils, we characterized Fe deficiency responses of three different isolates of Hedysarum carnosum, an endemic Tunisian extremophile species growing in native stands in salt and calcareous soil conditions. H. carnosum is a non-model crop. The three isolates, named according to their habitats Karkar, Thelja, and Douiret, differed in the expression of Fe deficiency symptoms like morphology, leaf chlorosis with compromised leaf chlorophyll content and photosynthetic capacity and leaf metal contents. Across these parameters Thelja was found to be tolerant, while Karkar and Douiret were susceptible to Fe deficiency stress. The three physiological and molecular indicators of the iron deficiency response in roots, Fe reductase activity, growth medium acidification and induction of the IRON-REGULATED TRANSPORTER1 homolog, indicated that all lines responded to -Fe, however, varied in the strength of the different responses. We conclude that the individual lines have distinct adaptation capacities to react to iron deficiency, presumably involving mechanisms of whole-plant iron homeostasis and internal metal distribution. The Fe deficiency tolerance of Thelja might be linked with adaptation to its natural habitat on calcareous soil.

The role of nodules in the tolerance of common bean to iron deficiency.

Iron is vital for the establishment and function of symbiotic root nodules of legumes. Although abundant in the environment, Fe is often a limiting nutrient for plant growth due to its low solubility and availability in some soils. We have studied the mechanism of iron uptake in the root nodules of common bean to evaluate the role of nodules in physiological responses to iron deficiency. Based on experiments using full or partial submergence of nodulated roots in the nutrient solution, our results show that the nodules were affected only slightly under iron deficiency, especially when the nodules were submerged in nutrient solution in the tolerant cultivar. In addition, fully submerged root nodules showed enhanced acidification of the nutrient solution and showed higher ferric chelate reductase activity than that of partially submerged roots in plants cultivated under Fe deficiency. The main results obtained in this work suggest that in addition to preferential Fe allocation from the root system to the nodules, this symbiotic organ probably develops some mechanisms to respond to iron deficiency. These mechanisms were implied especially in nodule Fe absorption efficiency and in the ability of this organ to take up Fe directly from the medium.

Immunolocalization of H(+)-ATPase and IRT1 enzymes in N(2)-fixing common bean nodules subjected to iron deficiency.

The demand for iron in leguminous plants increases during symbiosis, as the metal is utilised for the synthesis of various Fe-containing proteins in both plant and bacteroids. However, the acquisition of this micronutrient is problematic due to its low bioavailability at physiological pH under aerobic conditions. Induction of root Fe(III)-reductase activity is necessary for Fe uptake and can be coupled to the rhizosphere acidification capacity linked to the H(+)-ATPase activity. Fe uptake is related to the expression of a Fe(2+) transporter (IRT1). In order to verify the possible role of nodules in the acquisition of Fe directly from the soil solution, the localization of H(+)-ATPase and IRT1 was carried out in common bean nodules by immuno-histochemical analysis. The results showed that these proteins were particularly abundant in the central nitrogen-fixing zone of nodules, around the periphery of infected and uninfected cells as well as in the vascular bundle of control nodules. Under Fe deficiency an over-accumulation of H(+)-ATPase and IRT1 proteins was observed especially around the cortex cells of nodules. The results obtained in this study suggest that the increase in these proteins is differentially localized in nodules of Fe-deficient plants when compared to the Fe-sufficient condition and cast new light on the possible involvement of nodules in the direct acquisition of Fe from the nutrient solution.

Metabolic changes of iron uptake in N(2)-fixing common bean nodules during iron deficiency.

Iron is an important nutrient in N(2)-fixing legume nodules. The demand for this micronutrient increases during the symbiosis establishment, where the metal is utilized for the synthesis of various iron-containing proteins in both the plant and the bacteroid. Unfortunately, in spite of its importance, iron is poorly available to plant uptake since its solubility is very low when in its oxidized form Fe(III). In the present study, the effect of iron deficiency on the activity of some proteins involved in Strategy I response, such as Fe-chelate reductase (FC-R), H(+)-ATPase, and phosphoenolpyruvate carboxylase (PEPC) and the protein level of iron regulated transporter (IRT1) and H(+)-ATPase proteins has been investigated in both roots and nodules of a tolerant (Flamingo) and a susceptible (Coco blanc) cultivar of common bean plants. The main results of this study show that the symbiotic tolerance of Flamingo can be ascribed to a greater increase in the FC-R and H(+)-ATPase activities in both roots and nodules, leading to a more efficient Fe supply to nodulating tissues. The strong increase in PEPC activity and organic acid content, in the Flamingo root nodules, suggests that under iron deficiency nodules can modify their metabolism in order to sustain those activities necessary to acquire Fe directly from the soil solution.