[Microbiological evaluation of the effectiveness of gynalgin in the treatment of vaginitis]. / Ocena mikrobiologiczna skutecznosci Gynalginu w zapaleniach pochwy u kobiet.

The microbiological effectiveness of the preparation Gynalgin produced by POLFA Pharmaceutical Works in Rzeszów was assessed in cases of vulvovaginitis in 55 patients with clinically diagnosed inflammatory conditions of the lower genital tract, who were given Gynalgin tablets in 10-day courses. Vaginal smears were examined three times for the presence of bacteria, fungi and trichomonas vaginalis (before and immediately after the treatment, and two weeks later). In the initial examination in five vaginal smears mixed bacterial flora was found, in 6 smears trichomonas was present, in 4--bacteria and fungi, and in one--trichomonas and fungi. After the treatment in control examinations I and II the number of the isolated bacterial strains was lower, trichomonas was no longer present, and the number of fungi was reduced evidently. In the light of these microbiological examinations Gynalgin was found to exert a strong fungicidal, bactericidal and antitrichomonal activity, and the results of laboratory investigations agreed with those of clinical trials of Gynalgin effectiveness.

[Content of essential metals in colostrum of women delivered by cesarean section in early puerperium]. / Zawartosc metali esencjalnych w siarze poloznic po cieciu cesarskim we wczesnym okresie pologu.

The concentrations of magnesium, iron, copper and zinc were determined by atomic absorption spectrometry in the colostrum of 5 women in puerperium after delivery by Cesarean section. The samples of colostrum were obtained for examination between 11 and 12 hours on puerperium days from 4th to 7th. A significant rise of the concentration between these days was noted only in the case of iron (p less than 0.05). The differences in the levels of other metals over time period were random. The daily supply of these elements with milk to the breast-fed newborn increased evidently in that time period. Statistically significant differences were noted between days 4 and 6 (p less than 0.05) and the successive days (p less than 0.01). The daily supply of magnesium estimated on the 8th day of puerperium was not exceeding 50% of the lower limit of the recommended daily supply of this metal for newborns and infants. The contents of iron and copper in the daily portion of colostrum were at the borderline of the recommended values, and only in the case of zinc they fell within the range of these values.

The intrapartum content of toxic metals in maternal blood and umbilical cord blood.

The concentrations of cadmium, lead and total mercury were determined by atomic absorption spectrometry in maternal blood and umbilical cord blood collected during the III-rd stage of term delivery from 56 mother-neonate pairs. Cadmium and mercury levels measured on both sides of placenta did not differ significantly while lead levels in maternal blood were significantly higher than its cord blood values. In all three metals studied, the concentrations in maternal blood strongly correlated with the corresponding cord blood values. A significant, positive correlation was established between the parity of the examined women and the umbilical cord blood contents of lead and mercury. The obtained results support the opinion that human placenta does not form an effective barrier to toxic metal intake by the fetus.

[Bartlett's test in the statistical determination of amino acids using an automatic analyzer]. / Zastosowanie testu Bartletta do staystycznej charakterystyki oznaczania aminokwasów za pomoca automatycznego analizatora

Bartlett's test was applied to estimate the precision of the determination method of amino acids using the automatic analyser "Unichrom". The calculations were done on the basis of the results obtained from determinations of the amino acids in 8 different materials of plant and animal origin. No significant differences among standard deviation of 11 amino acids were found; this allowed the authors to express the precision of the method for these amino acids by mean standard deviation. In order to express the error of the method in relative values (variation coefficient), the analysis of variance was carried out, which showed a significance of differences in the mean levels for all amino acids. Confidence intervals of the mean levels of the amino acids determined by the Duncan test allowed us to form groups of materials in which the levels of the individual amino acids were similar. The variation coeffitients (the error of the method expressed in percentage) were calculated for the material groups which did not differ significantly in regard to the mean content of the given amino acid. In the majority of cases the error of the method for determining amino acids was about 5%. As a rule, it was higher only for threonine and tyrosine, and it was from 5 to 9% in the particular materials.