[Prenatal diagnostics of Toxoplasma gondii invasion by quantitative method of PCR-TaqMann]. / Prenatalna diagnostyka zarazenia pierwotniakiem Toxoplasma gondii metoda ilosciowej polimerazowej reakcji lancuchowej PCR-TaqMann.

Routine serological diagnosis of toxoplasmosis provides high sensitivity, but not high specificity. The high sensivity combined with high specifity offered by PCR-TaqMann as well as the degree of infection led us to investigate the presence and levels of T. gondii DNA in amniotic fluid, maternal and neonatal blood in cases of pregnancy where infection with this agent was suspected. Samples of amniotic fluid and blood were taken from pregnant women. Postnatal blood samples were also taken from their infants. Presence and levels of toxoplasma DNA was investigated using PCR-TaqMann. PCR products were detected by electrophoresis on polyacrylamide gel. The PCR-TaqMann test is highly sensitive, specific and useful method allowing detection of the parasite genome and assessement of its level.

[Detection of Toxoplasma gondii DNA by PCR in mother's blood, amniotic fluid and child's blood in selected cases of pathological pregnancy]. / Wykrywanie DNA Toxoplasma gondii metoda polimerazowej reakcji lancuchowej (PCR) we krwi matki, plynie owodniowym i krwi noworodka w wybranych przypadkach patologii ciazy.

Prenatal screening of Toxoplasma gondii infection is controversial. The diagnosis is based on serological tests detecting IgM and IgG antibodies against T. gondii, but interpretation of these tests results is often confusing. It is commonly made retrospectively when serological screening indicates a possibility of recent infection. Most women have antibodies against T. gondii and serial testing is required only in monitoring of pregnancies where initial screening is negative. The introduction of Polymerase Chain Reaction (PCR) assay for detection of Toxoplasma gondii DNA in selected cases of pathologic pregnancies has permitted a more accurate and faster diagnosis of congenital toxoplasmosis infections.

[Usefulness of quantitative assessment of Toxoplasma gondii genome using PCR-TaqMan in amniotic fluid, maternal and neonatal blood in selected complications in pregnancy]. / Przydatnosc ilosciowej oceny genomu Toxoplasma gondii przy uzyciu metody PCR TaqMan w plynie owodniowym, krwi matki oraz krwi noworodków w wybranych przypadkach patologii ciazy.

UNLABELLED: Routine serological diagnosis of Toxoplasmosis provides high sensitivity, but not high specificity. The high sensitivity combined with high specificity offered by PCR-TaqMan as well as the degree of infection led us to investigate the presence and levels of Toxoplasma gondii genome in amniotic fluid, maternal and neonatal blood in cases of pregnancy where infection with this agent was suspected. MATERIALS AND METHODS: Samples of amniotic fluid and blood were taken from 28 women between the 16th and 40th week of gestational age. Postnatal blood samples were also taken from their infants. Included in the study group were women with IUGR, PROM, preterm delivery imminent, Toxoplasmosis in previous pregnancies, raised IgG or IgM anti-Toxoplasma antibody titers and with amniotic fluid disturbances (oligohydramnios and polyhydramnios). Presence and levels of Toxoplasma genome was investigated using PCR-TaqMan. PCR products were detected by electrophoresis on polyacrylamide gel. RESULTS: Toxoplasma gondii genome was detected in blood from 13 women, 3 newborns and in amniotic fluid from one other women. Toxoplasma genome was detected in blood from one newborn, but was not detected in sample from its mother. CONCLUSIONS: The PCR-TaqMan test is highly sensitive and specific method allowing detection of the parasite genome and assessment of its level. Limitations of this method are its relatively high cost and poor access to ABI PRISM 7700 (TaqMan) sequence detector. The PCR TaqMan is useful in cases, where serological tests for the presence of infections are ambiguous.

[A cause of fetal ascites during intrauterine ileus with perforatio intesine]. / Przypadek izolowanego wodobrzusza plodu, w przebiegu wewnatrzmacicznej niedroznosci jelit z perforacja.

Fetal ascites may be caused by different abnormalities. Determining of the cause is very important in terms and the way of treatment. Some cases may require immediate interaction. We presented fetal ascites with atresio intensive with intrauterine perforation--the way of diagnosis and success of therapy.

[Congenital defects in the newborn--difficulties in prenatal diagnosis]. / Wady rozwojowe noworodków--trudnosci w diagnostyce prenatalnej.

The aim of this work is an analysis of developmental defects in newborn infants, born in the Clinical Department of Pregnancy Pathology and Reproduction in Zabrze, during the 19 months of its existence. Difficulties in prenatal diagnosis of developmental defects are presented and their structure is discussed. The study included 1524 live born infants with birth weight over 500 g and 12 stillborn. In the live born group developmental defects were found in 71 (4.6%) infants. Among the 12 stillborn infants developmental defects were present in one child (8.3%). The most common developmental defects, in our material, were those of the skeletal system and heart defects. Prenatally, developmental defects were diagnosed in 8% of the examined foetuses. The quality of health care in pregnant mothers of the investigated newborn infants was assessed, with relation to medical examination, ultrasonographic scan and biochemical screening tests. In many cases, modern standards of management were not maintained.