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Klin Lab Diagn ; (1): 17-20, 2008 Jan.
Article in Russian | MEDLINE | ID: mdl-18314774

ABSTRACT

Multiple myeloma is a malignant proliferative disease of plasma cells. Flow cytometric immunophenotyping makes it possible to identify a malignant clone of myeloma cells in the shortest possible time, to determine its phenotype, and differentiate transformed and preserved plasma cells. The article presents an immunophenotyping strategy using three-color monoclonal antibodies (CD35, CD14, CD38, CD138, and CD19) and an algorithm of verification of transformed plasma cells. Particular emphasis is placed on both the practical aspects of performing this assay and on the clinical application of the obtained results.


Subject(s)
Antigens, CD/analysis , Bone Marrow/immunology , Immunophenotyping/methods , Multiple Myeloma/diagnosis , Antibodies, Monoclonal/immunology , Bone Marrow/pathology , Flow Cytometry/methods , Humans , Multiple Myeloma/pathology
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