ABSTRACT
Bovine ligamentum nuchae elastin that had been partially digested by porcine pancreatic elastase and then thoroughly washed with 0.2 M-NaC1/0.05 M-sodium borate solution continued to release peptide fragments in solution. Complete removal of elastase from the elastin surface required extraction with dilute alkali, a procedure that does not irreversibly inactivate the enzyme or hydrolyse elastin.
Subject(s)
Elastin/analysis , Pancreatic Elastase/isolation & purification , Animals , Borates , Cattle , Kinetics , SolubilityABSTRACT
A new type of pH sensor suitable for chronic intra-vascular implantation by virtue of its small size, flexibility, and ruggedness was constructed and evaluated. The pH-sensitive element was a thin film of an elastromeric polymer made ion permselective to proteons by adding a lipophilic, specific H+-ion carrier. This was coated onto small diameter silver wires to form sensors. In preliminary trials in anesthetized dogs, the sensors permitted continuous, accurate in vivo blood pH measurement with rapid response (less than 0.1 s).