Potential interactions among disease, pesticides, water quality and adjacent land cover in amphibian habitats in the United States.

To investigate interactions among disease, pesticides, water quality, and adjacent land cover, we collected samples of water, sediment, and frog tissue from 21 sites in 7 States in the United States (US) representing a variety of amphibian habitats. All samples were analyzed for >90 pesticides and pesticide degradates, and water and frogs were screened for the amphibian chytrid fungus Batrachochytrium dendrobatidis (Bd) using molecular methods. Pesticides and pesticide degradates were detected frequently in frog breeding habitats (water and sediment) as well as in frog tissue. Fungicides occurred more frequently in water, sediment, and tissue than was expected based upon their limited use relative to herbicides or insecticides. Pesticide occurrence in water or sediment was not a strong predictor of occurrence in tissue, but pesticide concentrations in tissue were correlated positively to agricultural and urban land, and negatively to forested land in 2-km buffers around the sites. Bd was detected in water at 45% of sites, and on 34% of swabbed frogs. Bd detections in water were not associated with differences in land use around sites, but sites with detections had colder water. Frogs that tested positive for Bd were associated with sites that had higher total fungicide concentrations in water and sediment, but lower insecticide concentrations in sediments relative to frogs that were Bd negative. Bd concentrations on frog swabs were positively correlated to dissolved organic carbon, and total nitrogen and phosphorus, and negatively correlated to pH and water temperature. Data were collected from a range of locations and amphibian habitats and represent some of the first field-collected information aimed at understanding the interactions between pesticides, land use, and amphibian disease. These interactions are of particular interest to conservation efforts as many amphibians live in altered habitats and may depend on wetlands embedded in these landscapes to survive.

Residues of toxaphene in insectivorous birds (Petrochelidon spp.) from the Rio Grande, Texas.

Although it has been documented that wildlife in the Rio Grande Valley (RGV) contain increased concentrations of organochlorine (OC) contaminants, particularly DDE, little has been published on residues of toxaphene throughout this major North American watershed. In this study, 28 liver composites from adult swallows (Petrochelidon spp.) collected along the Rio Grande from 1999 through 2000 were analyzed for toxaphene residues using congener-specific gas chromatography-electron-capture negative ionization-mass spectrometry. Estimated total toxaphene concentrations ranged from 12 to 260 ng/g wet wt and were highest in samples from the lower RGV near Llano Grande Lake in Hidalgo and Cameron counties (Texas). Toxaphene congener profiles were relatively invariant throughout the watershed and were dominated by 2,2,5-endo,6-exo,8,8,9,10-octachlorobornane (P-42a or B8-806) with lesser amounts of several other Cl(7)-Cl(9) compounds, many of which remain unidentified. Petrochelidon spp. liver profiles appear to be intermediate in complexity between those in invertebrates and fish (more complex) and mammals (less complex) and differs somewhat from those reported for other avian species. In addition to other legacy OC contaminants, toxaphene residues were most concentrated in the lower RGV and accumulated at up to hundreds of parts per billion in these insect-eating birds, underscoring their utility as avian bioindicators of persistent organic pollutants.

Interpreting nonracemic ratios of chiral organochlorines using naturally contaminated fish.

Although reports of nonracemic proportions of chiral organochlorine pollutants in the environment are widespread, the interpretation of such data is not well developed. Using GC/MS and a chiral stationary phase consisting of 25% tert-butyldimethylsilylated beta-cyclodextrin in PS086 (beta-BSCD), we followed the change in the enantiomeric signature of 2-exo,3-endo,6-exo,8,9,10-hexachlorobornane (B6-923) in naturally contaminated fish maintained under toxaphene-free conditions. Whereas the enantiomeric ratio (ER) of B6-923 was near racemic at the start of the elimination experiment, it had increased severalfold by the end of 60 d. On the basis of first-order kinetics, one enantiomer of B6-923 was eliminated twice as fast as its mirror image, resulting in half-lives of 7 and 13 d, respectively. Enantioselective elimination by our test fish (Fundulus sp.) strongly suggests active biotransformation of B6-923; however, bioprocessing throughput estimates suggest a very low in situ rate of natural attenuation. These results confirm that the relatively constant ERs observed for chiral organochlorines in a given species are the result of competing processes, e.g., uptake vs elimination. Our experiments also further illustrate the utility of enantioselective analysis in characterizing the biotransformation of persistent organochlorine pollutants.