Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
Biochimie ; 90(8): 1207-23, 2008 Aug.
Article in English | MEDLINE | ID: mdl-18343231

ABSTRACT

We report here the details of G4-FID (G-quadruplex fluorescent intercalator displacement), a simple method aiming at evaluating quadruplex-DNA binding affinity and quadruplex- over duplex-DNA selectivity of putative ligands. This assay is based on the loss of fluorescence upon displacement of thiazole orange from quadruplex- and duplex-DNA matrices. The original protocol was tested using various quadruplex- and duplex-DNA targets, and with a wide panel of G-quadruplex ligands belonging to different families (i.e. from quinacridines to metallo-organic ligands) likely to display various binding modes. The reliability of the assay is further supported by comparisons with FRET-melting and ESI-MS assays.


Subject(s)
DNA/chemistry , DNA/metabolism , G-Quadruplexes , Acridines/chemistry , Acridines/metabolism , Base Sequence , Benzothiazoles/metabolism , DNA/genetics , Ligands , Oligonucleotides/chemistry , Oligonucleotides/genetics , Oligonucleotides/metabolism , Organometallic Compounds/metabolism , Quinolines/metabolism , Quinolinium Compounds/metabolism , Salts/pharmacology , Sensitivity and Specificity , Time Factors
SELECTION OF CITATIONS
SEARCH DETAIL
...