ABSTRACT
The fact that antibiotics exert no effect on Mycobacterium tuberculosis may be attributable to the production of their persistent forms. A correlation was studied between the expression of the genes Rv3286c, Rv2626c, Rv2031, and Rv3133c and the phenotypical tolerance to antibiotics in relation to the physiological condition of M. bovis BCG. The anaerobic growth conditions ("Wayne dormancy model") were found to be attended by the increased expression of all the studied genes, by the higher tolerance to rifampicin, and by the decreased phenotypical resistance to metronidazole.
Subject(s)
Antibiotics, Antitubercular/pharmacology , Drug Resistance, Bacterial/genetics , Gene Expression/physiology , Genes, Bacterial/genetics , Metronidazole/pharmacology , Mycobacterium bovis/genetics , Rifampin/pharmacology , Animals , Colony Count, Microbial , DNA, Bacterial/genetics , Gene Expression/drug effects , Genes, Bacterial/drug effects , In Vitro Techniques , Mycobacterium bovis/drug effects , Mycobacterium bovis/growth & development , Phenotype , RNA, Messenger/genetics , Reverse Transcriptase Polymerase Chain ReactionABSTRACT
The type of human papilloma virus (HPV) was determined in 26 children aged between 1 year 10 months to 15 years 5 months suffering from recurrent respiratory papillomatosis (RRP). Polymerase chain reaction identified DNA of HPV type 6, 11, 16 and 18. HPV DNA was detected in all the patients including fifteen patients infected with HPV type 11; seven patients infected with HPV type 6; four children infected with HPV type 6 and 11. Types 16 and 18 of HPV were not detected. The analysis of RRP course has found that laryngeal papillomatosis runs a more aggressive course in cases with HPV type 11 infection than in those with HPV type 6.
