ANALYSIS OF THE BLOOD HYPERCOAGULATION RISK IN PATIENTS WITH ISCHEMIC ATHEROTHROMBOTIC STROKE DEPENDING OF THE VDR GENE POLYMORPHISMS.

OBJECTIVE: Aim: of our study was the analysis of the blood hypercoagulation risk in patients with ischemic atherotrombotic stroke depending of the VDR gene polymorphisms. PATIENTS AND METHODS: Materials and Methods: Blood of 170 patients with ischemic atherothrombotic stroke (IATS) and 124 healthy individuals (control group) was used for genotyping. Four polymorphisms (FokI, BsmI, ApaI, TaqI) of gene VDR were examined with PCR-RFLP methodology. Statistical analysis was performed by using SPSS-17.0 program. RESULTS: Results: Among patients with IATS who are carriers of the f/f genotype, FokI polymorphism of VDR gene by high thrombin time and a decrease in the rate of spontaneous fibrinolysis was registered. In individuals with the B/B genotype homozygous for the polymorphic variant, BsmI had significantly lower mean values of prothrombin and thrombin time and increased the rate of spontaneous fibrinolysis. The homozygotes for the A-allele ApaI polymorphism have 2.7 times higher risk of developing blood hypercoagulation than homozygotes for the a-allele was found. CONCLUSION: Conclusions: Biochemical signs of hypercoagulation syndrome among patients with IATS who are carriers of the f/f genotype of the FokI polymorphic variant and among B/B homozygotes of the BsmI polymorphic variant and homozygotes for the A-allele of the AрaI polymorphism of the VDR gene were registered.

Arterial hypertension associated with hyperuricemia: features of heart damage.

OBJECTIVE: The aim is the analysis of hyperuricemia influence on the heart features in patients with arterial hypertension. PATIENTS AND METHODS: Materials and methods: We include 75 patients with arterial hypertension which were divided in two groups according to the level of uric acid in the blood, 30 practically healthy people. Patients from the I group (n = 40) had arterial hypertension and coexistent hyperuricemia; ІІ (n = 35) - arterial hypertension. Left ventricular mass index was determined for left ventricular hypertrophy confirmation. We used clinical, anthropometric, biochemical, instrumental, statistical method. Serum uric acid level was observed by the reaction with uricase. Left ventricular mass index was calculated as left ventricular mass to body surface area ratio. The results were analyzed statistically by SPSS 21 and Graphpad. RESULTS: Results: Left ventricular mass index was significantly higher (р = 0,0498) in patients from the І group (109,7 ± 3,21) g/m2 comparable with the ІІ (97,6 ± 5,35) g/m2 and increased in proportion to the biggest level of uric acid (r = 0,31; p = 0,04) in patients with arterial hypertension and hyperuricemia. CONCLUSION: Conclusions: Concentric and excentric left ventricular hypertrophy, increased left ventricular mass index proportionally to uric acid levels (r = 0,31; p = 0,04) is the confirmation of important role of hyperuricemia in the left ventricular hypertrophy development in patients with arterial hypertension.

Indicators of left ventricle hypertrophy in patients with arterial hypertension combined with obesity and their interconnection with polymorphism of lys198asn genе of endothelin-1.

OBJECTIVE: The aim of the study was to find dependence of left ventricular hypertrophy indexes to polymorphism of Les198Asn gene endothelin-1 and BMI. PATIENTS AND METHODS: Materials and methods: We took research in 160 patients with arterial hypertension, using ECG and polymerase chain reaction (PCR). Groups were divided additionally according to BMI (body mass index). RESULTS: Results: It was found, that patients with obesity had their Left ventricular mass and hypertrophy left ventricular indexes higher, than in patients with normal and increased body weight. Carriers of Asn198Asn and Lys198Asn genotypes Left ventricular mass and hypertrophy left ventricular indexes are higher than in carriers of Lys198Lys genotype. CONCLUSION: Conclusions: It was determined that in patients-carriers of Asn198Asn genotype, Left ventricular mass (LVMI) and hypertrophy left ventricular indexes (LVMMI) were higher compared to patients-carriers of Lys198Lys and Lys198Asn type, both in men and women. The dependence of LVMI and LVMMI are shown to be higher in patients with obesity than in people with normal and increased body mass.