Biomimetic Use of Food-Waste Sources of Calcium Carbonate and Phosphate for Sustainable Materials-A Review.

Natural and renewable sources of calcium carbonate (CaCO3), also referred to as "biogenic" sources, are being increasingly investigated, as they are generated from a number of waste sources, in particular those from the food industry. The first and obvious application of biogenic calcium carbonate is in the production of cement, where CaCO3 represents the raw material for clinker. Overtime, other more added-value applications have been developed in the filling and modification of the properties of polymer composites, or in the development of biomaterials, where it is possible to transform calcium carbonate into calcium phosphate for the substitution of natural hydroxyapatite. In the majority of cases, the biological structure that is used for obtaining calcium carbonate is reduced to a powder, in which instance the granulometry distribution and the shape of the fragments represent a factor capable of influencing the effect of addition. As a result of this consideration, a number of studies also reflect on the specific characteristics of the different sources of the calcium carbonate obtained, while also referring to the species-dependent biological self-assembly process, which can be defined as a more "biomimetic" approach. In particular, a number of case studies are investigated in more depth, more specifically those involving snail shells, clam shells, mussel shells, oyster shells, eggshells, and cuttlefish bones.

Dual Anta-Inhibitors Targeting Protein Kinase CK1δ and A2A Adenosine Receptor Useful in Neurodegenerative Disorders.

Currently, the number of patients with neurodegenerative pathologies is estimated at over one million, with consequences also on the economic level. Several factors contribute to their development, including overexpression of A2A adenosine receptors (A2AAR) in microglial cells and up-regulation and post-translational alterations of some casein kinases (CK), among them, CK-1δ. The aim of the work was to study the activity of A2AAR and CK1δ in neurodegeneration using in-house synthesized A2A/CK1δ dual anta-inhibitors and to evaluate their intestinal absorption. Experiments were performed on N13 microglial cells, which were treated with a proinflammatory CK cocktail to simulate an inflammatory state typical of neurodegenerative diseases. Results showed that the dual anta-inhibitors have the ability to counteract the inflammatory state, even if compound 2 is more active than compound 1. In addition, compound 2 displayed an important antioxidant effect similar to the reference compound ZM241385. Since many known kinase inhibitors are very often unable to cross lipid bilayer membranes, the ability of A2A/CK1δ double anta-inhibitors to cross the intestinal barrier was investigated by an everted gut sac assay. HPLC analysis revealed that both compounds are able to cross the intestinal barrier, making them promising candidates for oral therapy.

One-Pot Synthesis of Silica-Coated Gold Nanostructures Loaded with Cyanine 5.5 for Cell Imaging by SERS Spectroscopy.

Anisotropic gold nanoparticles have been recognized as promising agents for medical diagnostics and cancer therapy due to their wide functionality, photothermal effect, and ability for optical signal amplification in the near-infrared range. In this work, a simple and rapid method for the preparation of bone-shaped gold nanoparticles coated with a dye-impregnated silica shell with an aminated surface is proposed. The possibility of further functionalization the nanostructures with a delivery vector using folic acid as an example is demonstrated. The average size of the resulting tags does not exceed 70 nm, meeting the criteria of cell endocytosis. The prepared tags exhibit surface-enhanced Raman scattering (SERS) spectra at excitation with lasers of 632.8 and 785 nm. Cell imaging is performed on HeLa cells based on the most pronounced SERS bands as a tracking signal. The obtained images, along with scanning electron microscopy of cell samples, revealed the tendency of tags to agglomerate during endocytosis followed by the "hot spots" effect. To evaluate the toxic and proliferative effect of the nanotags, an MTT assay was performed with two HeLa and HEP G2 cell lines. The results revealed higher viability for HEP G2 cells.

Comparative study of fluorescence core-shell nanotags with different morphology of gold core.

The development of compact and highly active plasmonic nanotags tuned on the first transparency window of biological tissues is under demand for cell imaging applications. The optical activity of bare plasmonic nanoparticles is determined by morphology but the more complex core-shell systems require experimental verification as a shell may change the expected trends. A comparative study of fluorescence core-shell nanotags with different morphology of gold core is presented in this work. Four types of gold nanoparticles (nanostars, nanobones, short and long nanorods), differing in the surface roughness were used for preparation of complex nanotags with a polymer shell containing cyanine 5.5 dye inside and surface functionalized with folic acid as a model delivery vector. The obtained core-shell nanotags were characterized with transmission electron microscopy, UV-Vis absorption spectroscopy and zeta potential measurements. Imaging performance of the obtained nanotags was studied with a fluorescence microscope on human pancreatic cancer cells, indicating a successful internalization of all nanotags by cancer cells and fluorescence intensity depending on the spectral overlap between the dye, plasmonic band of gold core and laser wavelength. The tags based on gold nanorods showed the brightest fluorescence among the studied systems. Scanning electron microscopy of the cells incubated with nanotags proved their internalization in membrane and cytoplasm. The cell viability assay showed reduced cytotoxicity and good biocompatibility up to the concentration enough for cell imaging. The obtained results suggested that compact core-shell nanotags can be used for targeting the folate receptor positive tumor cells.

Uplink NOMA-MIMO Systems with Rayleigh Power Distribution.

The article is devoted to multiple-input multiple-output antenna systems, also called MIMO systems, which are widely used in wireless communication systems. In this article we consider a case when the MIMO system works in overloaded mode. In this mode MIMO systems can be considered as a system with non-orthogonal multiple access NOMA. The main goal of this article is to analyze this interesting case using statistical computer simulation. Based on the analysis of the capacity of a discrete-continuous multiuser MIMO uplink channel, the possibility of such systems functioning in overload mode is proved.