[Plasmapheresis in combined treatment of acute rejection crises after kidney transplantation]. / Primenenie plasmafereza v kompleksnoi terapii ostrykh krizov ottorzheniia posle peresadki pochki.

The aim of the study was assessment of effectiveness and safety of extracorporeal procedures (plasmapheresis) in prevention and combined treatment of acute rejection crises (ARC) after kidney transplantation in patients at high risk to develop acute immunological conflicts in early postoperative period. 94 patients aged 21-56 years after allotransplantation of cadaver kidney (ATCK) entered the study. They were divided into two groups: group 1 patients (n = 47) received plasmapheresis procedures in combined preventive and therapeutic treatment of ARC; matched group 2 patients (n = 47, retrospective) received the same therapy but plasmapheresis. In both groups there were many patients with a high titer of pre-existing antibodies and massive hemotransfusions in the past. The analysis covered also recipients with the second ATCK (9 and 4 patients, respectively). After ATCK both groups received three-component immunosuppressive treatment (neoral, prednisolone, azatioprin and/or sell-cept). The analysis of the transplant and recipient 11-month survival has estimated that it was 93.6 and 91.5% for the recipients, respectively, and 91.5 and 76.5% for the transplanted kidney for group 1 and 2, respectively. The conclusion is that plasmapheresis in combined prevention and treatment of ARC by humoral type in sensitized patients (high titer of the pre-existing antibodies, repeat transplantations, hemotransfusions) is pathogenetically grounded and lowers the percentage of irreversible episodes of acute transplant rejection early after surgery.

[Recombinant Thermus thermophilus His6-DNA polymerase with reverse transcriptase activity]. / Rekombinantnaia His6-DNK-polimeraza Thermus thermophilus s aktivnost'iu obratnoi transkriptazy.

The Tth DNA polymerase genes from the thermophilic bacterium Thermus thermophilus (strans HB-8 and Tt-111) were cloned and expressed in Escherichia coli cells. The target protein contained a polyhistidine tag at the N-terminus that allowed its single-stage isolation by affinity chromatography on Ni-NTA-agarose. The isolated enzyme displayed both high DNA polymerase and reverse transcriptase activities.

[Membrane permeability in combined arterial hypertension and pathology of the digestive organs (based on epidemiological study data)]. / Sostoianie membrannoi pronitsaemosti pri sochetanii arterial'noi gipertonii s patologiei organov pishchevareniia (po dannym épidemiologicheskogo issledovaniia).

An epidemiological study of arterial hypertension and digestive diseases revealed 27% patients with concurrent abnormalities. It was shown that determination of membranous lesions by the rate of red blood cell Na-Li contratransport may be useful in the diagnosis of syntropy.

[Cloning the asd and lysC genes from Cornyebacterium glutamicum]. / Klonirovanie genov asd i LysC Corynebacterium glutamicum.

Plasmids carrying an asd gene from a mutant. S-(2-aminoaethyl)-L-cysteine resistant strain of Corynebacterium glutamicum were selected from a clonoteque constructed on a plasmid cloning vector pSL5 by complementation of asd mutation in Escherichia coli. Evidence has been obtained that the cloned chromosomal DNA fragment contains also a complete sequence for feed-back-resistant aspartokinase lysC gene.

[Study of the regulation of crp gene expression in Escherichia coli K12]. / Izuchenie reguliatsii ékspressii gena crp u Escherichia coli K12.

The regulation of crp gene expression by CRP-cAMP complex was studied in E. coli strain by the crp-lac operon fusion. F'141 crp+ episome decreased 5-7 fold the high level of crp-lac expression in crp strains while F'141 crp episome had no effect. The hybrid plasmid pCAP2 crp+ with the intact crp gene did not affect the crp gene expression level in crp mutants, though they had acquired the Crp+ phenotype just as they did in F'141 crp+ presence. The F'141 crp+ and pCAP2 crp+ combination in crp mutants also resulted in decrease of the crp gene expression comparable to the registered in the presence of the F'141 crp+ plasmid. Similar repression occurred only in cya+ strains but not in cya strains. The crp gene is supposed to possess negative regulation by CRP-cAMP complex with a complementary factor also necessary. The latter is evidently located in an E. coli chromosome site overlapped by F'141 episome.

[The epidemiological aspects of combined arterial hypertension and peptic ulcer]. / Epidemiologicheskie aspekty sochetaniia arterial'noi gipertonii i iazvennoi bolezni.

Altogether 598 workers and employees of an industrial enterprise underwent epidemiological examination. The age of the examined ranged within 30 to 49 years. Arterial hypertension was revealed in 14.2% and ulcer disease in 9% of the population. 1.5% of the population presented with associated pathology. Among risk factors of paramount importance was unbalanced nutrition and hypotensive treatment. Impairment of cell membranes also played a certain role in the occurrence of associated pathology.

[Study of the expression of the cya gene in Escherichia coli K12 on a model of the hybrid cya-lac operon]. / Izuchenie ékspressii gena cya u Escherichia coli K12 na modeli gibridnogo operona cya-lac.

Bacteriophage Mud1 (Ap, lac) was used to construct the cya-lac operon fusion in Escherichia coli chromosome. The maximal cya expression was observed by measuring the levels of beta-galactosidase in the strains lacking the cAMP. The inhibition of cya transcription was proportional either to the amount of cAMP added or to the copy number of cya gene in the cell. Functional cAMP receptor protein (CRP) was necessary for the effect. The cya gene expression was low in cAMP-independent crp mutant even in the absence of the cAMP.

[Isolation of mutants with impaired retroinhibition of histidine biosynthesis]. / Poluchenie mutantov s narushennym retroingibirovaniem biosinteza gistidina.

The Escherichia coli, strain possessing purF, deoD and add mutations converts exogenous adenine into guanine nucleotides exclusively by the pathway coupled with histidine biosynthesis. When grown on adenine, this strain demonstrated sensitivity to histidine, thus making it possible to select histidine-resistant hisGR mutants with ATP-phosphoribosyltransferase desensibilized for histidine. The hisGR mutations were obtained in two his operons introduced into the his operon-sensitive E. coli strain: his operon of Salmonella typhimurium incorporated in DNA and his operon of E. coli on the F'episome. In both cases, the hisGR mutants obtained were shown to excrete histidine.

[Cloning and expression of the histidine operon of Salmonella typhimurium in cells of Escherichia coli]. / Klonirovanie i ékspressiia gistidinovogo operona Salmonella typhimurium v kletkakh Escherichia coli.

The histidine operon of Salmonella typhimurium and its fragments were cloned in Escherichia coli cells on a multicopy plasmid. Expression of the cloned genes and histidine production by the variants possessing the hisG mutation which desensibilizes the ATP phosphoribosyl transferase for histidine were studied. Amplification of the complete operon including the hisG gene enables histidine accumulation of 2-3 g/l after 72 hours of fermentation.

[Metabolic regulation of the histidine operon in Escherichia coli and Salmonella typhimurium]. / Metabolicheskaia reguliatsiia gistidinovogo operona Escherichia coli i Salmonella typhimurium.

Expression of the histidine operon in Escherichia coli cells in contrast to the one in Salmonella typhimurium is changed proportionally to cells growth rate on the different carbon sources. The specific activity of histidinol-dehydrogenase is repressed by addition of 19 amino acids both in Escherichia coli and Salmonella typhimurium independent of the growth medium used. Using of Escherichia coli and Salmonella typhimurium strains containing the heterologous histidine operons made possible to demonstrate the dependence of the histidine operon metabolic regulation to be determined by the operon itself but not by the specificity of the recipient cells. ppGpp was shown to be a positive regulator of the histidine operon expression in Escherichia coli.

[Prospective epidemiologic study of arterial hypertension in an organized male population]. / Prospektivnoe épidemiolgicheskoe issledovanie arterial'noi gipertenzii v organizovannoi muzhskoi populiatsii.

The effect of active arterial-hypertension preventive programs on the epidemiologic situation was studied over a nine-year period, 1977 through 1985. In the first five years, the number of effectively treated cases increased, while essential hypertension morbidity associated with temporary disability diminished, as did mortality and the incidence of myocardial infarction and cerebral accidents among 40- to 54-year-old male industrial workers. After an active preventive program was abandoned at one factory, the parameters examined deteriorated considerably, and a sample selected for comparison showed an abrupt drop in treatment efficiency within three years after annual screenings were discontinued.

[Primary structure of the alpha-subunit of Na+,K+-ATPase from the swine kidney. III. Complete nucleotide sequence corresponding to the structural region of the gene]. / Pervichnaia struktura alpha-sub''edinitsy Na+,K+-ATP-azy pochek svin'i. III. Opredelenie polnoi nukleotidnoi posledovatel'nosti, sootvetstvuiushchei strukturnoi chasti gena.

The nucleotide sequence of the cDNA, containing coding region of the alpha-subunit of the pig kidney Na+, K+-ATPase, was determined. The region contains 3063 b.p. coding for 1021 amino acid residues. In the course of processing, five amino acid residues are cleaved to yield the mature Na+, K+-ATPase alpha-subunit containing 1016 amino acid residues.

[Genes encoding the beta-subunit of bacterial RNA-polymerases. I. Primary structure of the EcoRI-C fragment of the Salmonella typhimurium gene rpoB]. / Geny, kodiruiushchie beta-sub''edinitsu RNK-polimeraz bakterii. I. Pervichnaia struktura EcoRI-C-fragmenta gena rpoB Salmonella typhimurium.

BglII fragment of S. typhimurium DNA, containing rpoB gene coding for the RNA polymerase beta-subunit, was cloned. The nucleotide sequence of the rpoB gene EcoRI-C fragment (2873 b.p.) was determined.

[Construction of the hybrid crp-lac operon and study of the role of CRP-cAMP complex in its regulation in Escherichia coli]. / Konstruirovanie gibridnogo operona crp-lac i izuchenie roli kompleksa CRP-tsAMF v ego reguliatsii u Escherichia coli.

Phage Mu d1(Ap,lac) was used to construct the hybrid crp-lac operon in Escherichia coli cells possessing crps mutation which increases sensitivity of bacterial growth to exogenous cAMP. Transcription of the structural lac genes in this hybrid operon was initiated from the promoter region of the crp gene. By measuring levels of beta-galactosidase enzyme, the maximal transcription of the crp gene was obtained in strains with cya or crp mutations causing inactivation of the CRP - cAMP complex. Restoration of cya+crp+ genotype resulted in 5 to 7 times decrease of the lacZ gene activity. At the concentration of exogenous cAMP which induces catabolic derepression in the wild-type strain, the expression of crp-lac operon increased 2-3 times. Using F'ts114lac-mediated chromosome mobilization, the direction of transcription of the crp gene was found to be counterclockwise, relative to the E. coli genetic map.

[Prospective epidemiological study and the effectiveness of the prevention of arterial hypertension in an organized female population]. / Prospektivnoe épidemiologicheskoe issledovanie i éffektivnost' profilaktiki arterial'noi gipertenzii v zhenskoi organizovannoi populiatsii.

Altogether 3875 women aged 30 to 50 engaged in the textile industry, were examined. Primary prevalence of arterial hypertension was 7.0-7.2%. The implementation of a special program of active prophylaxis resulted in a significant rise of the number of effectively treated patients as compared to the control group.

[Protective effect of alpha-tocopherol on the hydroxylating system in liver endoplasmic reticulum membranes against the damaging activity of hyperbaric oxygenation]. / Zashchita al'fa-tokoferolom gidroksiliruiushchei sistemy membran éndoplazmaticheskom retikuluma pecheni ot povrezhdaiushchego deistviia giperbaricheskoi oksigenatsii.

Effect of hyperbaric oxygenation (oxygen pressure 3039 hPa, exposition 3 hrs) on activity of the hydroxylating system and lipid peroxidation was studied in membranes of rat liver endoplasmic reticulum. Within the first 3 hrs after hyperbaric oxygenation the rates of aniline n-hydroxylation, amidopyrine N-demethylation, NADPH oxidation and the cytochrome P-450 level were decreased, while the rates of ascorbate- and NADPH-dependent lipid peroxidation were increased. Oil emulsion of alpha-tocopherol, administered per os into rats at a dose of 50 mg/kg every 12 hrs within 2 days before hyperbaric oxygenation, prevented the posthyperoxic deterioration of xenobiotic metabolism and the intensification of lipid peroxidation in liver microsomes.