[The choice of antibiotic according the results of standard bacteriological analysis and express-method without isolation of pure cultures.]

The efficiency of choosing antibiotics using two methods was analyzed. The first method is based on standard laboratory analysis including isolation of pure cultures of prospective agent and detection of their individual sensitivity to antibiotics. The second method is based on a new express-technique permitting to simultaneously evaluate effect of pharmaceuticals on cultivated and not cultivated yet bacteria without isolation of pure cultures. At that, the second method permits to determine efficiency of antibiotics namely in those concentrations that can be made in the given nidus of infection. The study demonstrated existence of discrepancy between results obtained by using the given methods. The cause of discrepancy can be presence of not cultivated yet bacteria in pathologic sample. In favor of this assumption testify the received data concerning samples from patients where were presented a significant number of spore-former bacteria having additional mechanisms of resistance to antibiotics. Furthermore, the implemented study established antibiotics characterized by the greatest discrepancy of results obtained by applied methods that indicates the necessity of revision of schemes of implementation of start or empiric therapy.

[The choice of antibiotic in microbiological analysis of phlegm.]

The efficiency of application of the test-system "Vy`borAntibiotika" (AntibioticChoice) in incubation of a maximal possible number of bacteria from pathologic material in case of pneumonia was studied. The results of meta-genome analysis permitted to establish that test-system support incubation of practically all bacteria detected in phlegm, including those attributed to so far non-incubated ones. The comparison of the results was carried out concerning a standard detection of sensitivity of bacteria to antibiotics and choice of efficient medicinal according the results of application of test-system "Vy`borAntibiotika". The obtained data demonstrates that test-system permits to choose antibiotic during 6-20 hours without isolation of pure strain.

[The choice of antibiotic in microbiological study of pyoinflammatory processes.]

The efficiency of application of test-system "Antibiotic Choice" was examined concerning evaluation of sensitivity to medications of maximal possible number of bacteria from pathological samples at burn trauma without isolation of pure culture. The results of metagenome analysis demonstrated that test-system permits supporting factually all bacteria discovered in wound discharge, including ones related to not cultivated yet. The comparison was carried out concerning results of standard identification of sensitivity of bacteria to antibiotics and efficient medication according the results of application of test-system "Antibiotic Choice". The obtained results demonstrate that test-system permits choosing antibiotic during 6-20 hours without separation of pure culture.

[CHOOSING ANTIBIOTIC IN MICROBIOLOGICAL ANALYSIS OF INFECTIONS OF URINARY EXCRETION SYSTEM].

The effectiveness of application of test-system "Choice of antibiotic" was evaluated as a tool for incubation of maximal amount of bacteria from pathological material under acute cystitis. The results of meta-genome analysis established that test-system permits supporting growth of practically all bacteria detected in urine, including ones relating to "uncultivated for the present". The comparison of results of standard detection of sensitivity of bacteria to antibiotics and identification of effective pharmaceutical according the results of application of test-system "Choice of antibiotic" as well was implemented It is demonstrated that test- system permits choosing antibiotic during 6-20 hours wiihout isolation of pure strain.

[Theoretic scheme of immunoenzyme analysis (the "sandwich" variant)]. / Teoreticheskaia skhema immunofermentnogo analiza (variant "sandvich").

The sandwich variant of the enzyme-linked immunosorbent assay (ELISA) is a simple serological reaction of three components, and the antigen-antibody interaction follows the law of active masses. To increase the sensitivity of ELISA with respect to the antigen, the plates should be sensitized with moderate concentrations of antibodies and the confugate should be introduced at relatively low concentrations. To increase the activity of the conjugate, the plates should be sensitized with high concentrations of antibodies, but in this case the sensitivity of ELISA will not be high.

[Determination of embryotoxic and teratogenic effect of a new antidepressive agent, pyrazidol]. / Opredelenie embriotoksicheskogo i teratogennogo deistviia novogo antidepressanta pirazidola.

The effect of the new original antidepressant pyrasidol on the embryogenesis of albino rats was investigated. With a single introduction of the drug with a tube into the stomach in a dose of 200 mg/kg within the first 15 days of pregnancy pyradisol was found to produce no embrytoxic and teratogenic effects. Fluorescent microscopy revealed the presence of the pyrasidol metabolites in the amniotic fluid of the test animals 2 hours after enteral administration of the drug, on the 15th and 20th days of pregnancy.

[Influence of antibiotics of the penicillin series on the fetal and newborn development of rats]. / Vliianie antibiotikov penitsillinovogo riada na razvitie ploda i novorozhdennogo u krys

Penicillin injected to rats in high doses at various periods of pregnancy produced no negative effect on the devloping fetus and neonate. At the same time bicillin-3 produced resorption of the embryonic cells and had a negative effect on the heart development.

[Intracellular growth of actinophage in Actinomyces lividans culture lysogenic heat-induced mutant]. / Vnutrikletochnoe razvitie aktinofaga v kul'ture Actinomyces lividans, lizogennoi po termoindutsiruemomu mutantu

Intracellular growth of a thermoinduced prophage was caused by the action of elevated temperature on the lysogenic culture of Actinomyces lividans 66 (phiC31 ct22). The growth took place in the nucleoid where loose, oval or round, particles consisting of twisted NA threads were formed. Later, electron-dense phage heads, covered with a sheath, appeared, and still later--phage tails. Thermoinduction was terminated by lysis of the mycelium of the actinomycete when mature particles of the actinophage were liberated into a surrounding medium.