[Metabolic correction of bile outflow in patients with obstructive jaundice].

UNLABELLED: The purpose of research is to study the influence of infusion therapy structure on the of metabolic disorders and biliary excretion correction in patients with obstructive jaundice. MATERIALS AND METHODS: The study was carried out in 139 patients divided into 3 groups with the same volume of infusion therapy (1600 ml/day). In 1-St group (54 patients) infusion therapy included ringer's lactate and 10% glucose solution in the ratio of 1:1. In the 2nd group (37 patients) infusion therapy was sterofundine - G-5. In the 3rd group (48 patients) in infusion therapy have included remaxol with dosage of 800 ml/day. RESULTS: The inclusion of remaxol and sterofundin in infusion therapy leads to the improvement of biliary excretion in the early postoperative period versus ringer's lactate and 10% glucose solution. Remaxol has the advantage in metabolic disorders and biliary excretion correction versus sterofundine-G-5.

The properties of human DNA fingerprints produced by polymeric monocore probes (PMC probes).

The properties of human DNA fingerprints detected by multilocus polymeric monocore probes (PMC probes) have been investigated. The PMC probes were produced by the polymerase chain reaction with two partly complementary oligonucleotides homologous to various minisatellite or microsatellite core sequences (Ijdo J, Wells RA, Baldini A, Reeders ST. Nucleic Acids Res 1991;19:4780). It has been shown that these probes possess increased sensitivity, they detect considerably more hypervariable fragments in genomic DNA thus exhibiting advantages over the corresponding oligonucleotides and natural polycore minisatellite probes. Variation in the DNA fingerprints of different individuals produced by these probes indicates that the probability of accidental identity is very low (< 10(-12)). According to the data of cross-hybridization with PMC probes of various specificity, several distinct families can be distinguished among G-rich hypervariable sequences of the human genome.

[Collagen type IV of the basal membrane of the epithelium of the skin in certain forms of pathology]. / Kollagen IV tipa bazal'noi membrany épiteliia kozhi pri nekotorykh formakh patologii.

Using immunofluorescence, attenuation or complete disappearance of type IV collagen antiserum reaction were shown in the epidermal basal membrane zone of SLE patients. Some positive material was revealed in the intercellular spaces of the epidermis. There is an inverse correlation between the amount of immune complexes in the dermo-epidermal region and the intensity of anticollagen serum reaction in the basal membrane zone. This can be explained by the toxic effect of immune complexes on collagen-synthesizing cells and the disturbance of this protein fixation in the basal membrane followed by its sequestration from the affected regions. Complex therapy including hemoperfusion restores normal collagen location in the epidermal basal membrane.