ABSTRACT
In this work the comparative data on the antigenic schemes of P. aeruginosa proposed by different authors are presented. The scheme of serological grouping, accepted in the USSR, has been brought into accord with the current nomenclature of O-antigens, proposed by Lányi and Bergan. The O-antigen composition of the typing strains belonging to groups 3 and 9 according to the classification of Lányi-Bergan has been deciphered, which allows one to extend the antigenic scheme of P. aeruginosa by introducing new antigenic variants.
Subject(s)
Pseudomonas aeruginosa/classification , Antigens, Bacterial/classification , Serotyping/methods , Terminology as Topic , USSRABSTRACT
The authors carried out serological typing of 98 Pseudomonas aeruginosa strains, isolated from patients of burn department of the Sklifosovsky First Aid Institute in January-July, 1974, and of 215 strains obtained from other sources; their sensitivity to 13 antibiotics was determined. Pseudomonas aeruginosa cultures isolated from the patients were typed with O-sera of 10 serological types. The presence of several hospital strains of Pseudomonas aeruginosa was found by means of serological typing; along with these there were revealed cultures of this causative agent sporadically appearing in the department. Sensitivity to some antibiotics could serve as an additional criterion for differentiation of Pseudomonas aeruginosa strains of the same serological type.
Subject(s)
Pseudomonas Infections/epidemiology , Pseudomonas aeruginosa/isolation & purification , Adult , Aged , Burns/complications , Drug Resistance, Microbial , Epidemiologic Methods , Humans , Male , Middle Aged , Pseudomonas Infections/complications , Pseudomonas Infections/microbiology , Pseudomonas aeruginosa/classification , SerotypingABSTRACT
Materials of clinico-bacteriological study of 302 patients with thermal burns of different severity pointed to a considerable elevation of the incidence of Ps. aeruginosa isolation (in cultures) from burn wounds; the last few years it was found in about 50% of the cases. The greater frequency of Ps. aeruginosa detection correlated with the increase of the severity of the affection. By using Soviet set of 17 type agglutinating sera it was possible to type almost 90% of the Ps. aeruginosa cultures isolated from the wounds; a marked prevalence (over 70% of the strains) of cultures belonging to the serological group II was revealed. Patients admitted to the burn centre at early periods after the trauma displayed infection of the wound with the Ps. aeruginosa strain of the II serological group.
Subject(s)
Burns/complications , Pseudomonas Infections/complications , Wound Infection/etiology , Humans , Pseudomonas Infections/microbiology , Pseudomonas aeruginosa/isolation & purification , Serotyping , Wound Infection/microbiologyABSTRACT
A total of 552 strains of Pseudomonas aeruginosa were obtained in 1970-1975 from different hospitals; the strains were isolated from various sources. Serotyping of these cultures was carried out with the aid of a set of agglutinating sera (20 in all) prepared by the author, and 97.5% of these cultures were typed. A high percentage of the strains belonging to the II, III, and IV serological groups and to the serotype 011 pointed to the epidemiological significance of these serological groups. The results obtained showed the expediency of introduction of the method of serological typing of the Ps. aeruginosa cultures into the public health practice for the purpose of epidemiological analysis.
Subject(s)
Pseudomonas Infections/microbiology , Pseudomonas aeruginosa/immunology , Humans , Pseudomonas aeruginosa/isolation & purification , Serotyping , USSRABSTRACT
The authors used the method of indirect immunofluorescence for detection and serological typing of Ps. aeruginosa in pathological material. This method was found to be useful for detection of Ps. aeruginosa in association with other microorganisms. The indirect immunofluorescence method can be used for determination of the serological group of the clinical Ps. aeruginosa strains.
Subject(s)
Fluorescent Antibody Technique , Pseudomonas aeruginosa/classification , Serotyping/methods , Humans , Pseudomonas Infections/microbiologyABSTRACT
The authors elaborated a method of obtaining sorbed specific sera for serotyping of Ps. aeruginosa on slide. A set of specific sera consisting of 20 sera was prepared; 93% of 500 cultures isolated from pathological material were typed with the aid of this set.
Subject(s)
Antigens, Bacterial/analysis , Pseudomonas aeruginosa/immunology , Serotyping/methodsABSTRACT
Seven antibiotics, such as gentamycin, kanamycin, streptomycin, polymyxin M, carbenicillin, tetracycline and rifampicin were studied with respect to their effect on 260 strains of Ps. aeruginosa isolated from various sources within 1945-1971. Gentamycin and polymyxin M proved to be most active in vitro. Carbenicillin showed moderate activity. The sensitivity levels of the cultures of Ps. aeruginosa isolated from various sources within 30 years were identical. Serological typing of 59 cultures of Ps. aeruginosa was performed and their virulence was studied in parallel with their antibiotic sensitivity testing. No correlation between the antibiograms, serotypes and virulence of Ps. aeruginosa was found.
Subject(s)
Anti-Bacterial Agents/pharmacology , Pseudomonas aeruginosa/drug effects , Aminoglycosides/pharmacology , Animals , Culture Media , Humans , Mice , Microbial Sensitivity Tests , Serotyping , Snakes/microbiology , VirulenceABSTRACT
A method of preparation of antigens of Verder and Evans and a scheme of rabbit immunization suggested by them was used to obtain agglutinating specific O-sera to 12 type Ps. aeruginosa strains of Bulgarian and London collections. There was shown a principal correlation of the O-types of Ps. aeruginosa cultures of both collections. A possibility of using the sera obtained for typing the Ps. aeruginosa on glass was confirmed. Of 80 museum cultures 52 strains were typed; among them type 0-6 was frequently encountered.