[Membrane-filtration immunoassay: reagents, methods and the diagnostic and technical means for detection]. / Membranno-fil'tratsionnyi immunoanaliz: reagenty, metody, diagnosticheskie i tekhnicheskie sredstva indikatsii.

The comprehensive development of dot-EIA made at the State Research Institute of Biological Instrument-Making Industry has provided devices KIMF-02 and KIMF-03), a base of chemical reagents, immunoassays, test systems for detection of a wide range of causative agents of viral and bacterial infections, that of serodiagnosis of their related diseases. The KIMF-02 kit has undergone engineering and medical tests and recommended for the Ministry of Health of the Russian Federation to produce them in stock. The kit includes all required for analysis even in an ill-equipped laboratory, a set of attached agents ensures a valid visual recording of results. The developed procedures and test systems allow the immunoassay to be as sensitive as TIFA; however, they are laborious and much simpler in design. The simple and rapid procedures of dot-EIA are recommended for incorporation into the a package of laboratory methods for verification of the accumulation of virus-specific antigens in various biological substrata, environmental samples, for control of the activity of antigens and antibodies used in serological tests, for detection of specific antigens in the clinical samples, and for serodiagnosis of infections.

[Diagnostic test kit for detection of antibodies to syphilis agent]. / Diagnosticheskaia test-sistema dlia vyiavleniia antitel k vozbuditeliu sifilisa.

A diagnostic enzyme immunoassay test kit is proposed for detecting anti-treponema antibodies in sera of syphilis patients, usable in two variants: on prefabricated polystyrene plates or by immunofiltration using a membrane filtration enzyme immunoassay kit. A peculiar feature of the test kit is the use of enzyme immunoassay conjugate based on monoclonal antibodies to light chains of human immunoglobulins, highly purified antigen from cultural T. pallidum, two substrates for detecting enzymatic activity. The efficacy of the test was confirmed on 163 patients' and control sera and compared with that of the complement fixation test, two modifications of immunofluorescent test, and micromodification of the precipitation test. The test proved to be highly sensitive, specific, and reproducible, and is recommended as a confirmation test for the diagnosis of syphilis.