ABSTRACT
The experimental simulation of ovary hyperstimulation syndrome (OHSS) in test murine by administration with several dozes of exogenous gonadotropic hormones has been performed. It has been shown that mouse ovary hormone stimulation leads to manifestation of all OHSS symptoms: ovary swelling and hyperemia, injury of their structure, cystic corpus luteum formation, hemorrhagic follicles, folliculogenesis disturbance, oocyte and embryo morphology injury. The degree of OHSS symptom manifestation depends on hormone doze. On the third day after human chorionic gonadotropin administration all OHSS symptoms take place as an evidence of this syndrome prolonged character.
Subject(s)
Oocytes/pathology , Ovarian Hyperstimulation Syndrome/pathology , Ovary/pathology , Animals , Chimera , Chorionic Gonadotropin/pharmacology , Disease Models, Animal , Dose-Response Relationship, Drug , Embryo, Mammalian , Female , Gonadotropins, Equine/pharmacology , Injections, Intraperitoneal , Mice , Mice, Inbred C57BL , Mice, Inbred CBA , Oocytes/drug effects , Oocytes/growth & development , Organ Size/drug effects , Ovarian Hyperstimulation Syndrome/chemically induced , Ovarian Hyperstimulation Syndrome/embryology , Ovary/drug effects , Ovary/growth & development , Ovulation Induction/methodsABSTRACT
Intracellular concentrations of potassium and phosphorus were determined by Electron Probe Microanalysis in mouse mature oocytes and zygotes. The oocytes were characterized by insignificant variations in the concentrations of these elements in the cytoplasm: 60 +/- 4 and 103 +/- 6 mM, respectively. In zygotes, on the contrary, significant variations were observed: 64 +/- 16 and 84 +/- 14 mM, respectively. Changes in the potassium homeostasis during the first cell cycle have been discussed.
