ABSTRACT
A total of 187 parturients (66 with a history of measles and 121 immunized with live measles vaccine, or LMV, in childhood) and their 187 newborn infants, as well as 195 children aged up to 1 year, were examined. Antimeasles antibodies in blood sera were detected in the hemagglutination inhibition test. In all mothers with a history of measles and in their newborn infants antimeasles antibodies in different titers were detected. In mothers, formerly immunized with LMV, antimeasles antibodies were absent in 5.8% and in their newborn infants, in 6.6% of the examinees. Among children aged up to 1 year, born of formerly immunized mothers, more rapid disappearance of passive antimeasles immunity was observed. In cases of contact with measles, the serological examinations of all children born of mothers immunized with LMV should be carried out in order to protect seronegative children by passive or active immunization.
Subject(s)
Maternal-Fetal Exchange/immunology , Measles/immunology , Adolescent , Adult , Antibodies, Viral/blood , Female , Hemagglutination Inhibition Tests , Humans , Infant , Infant, Newborn , Measles/prevention & control , Measles Vaccine/immunology , Measles virus/immunology , Pregnancy , Russia , Urban PopulationABSTRACT
The immune structure of the Moscow City population (mostly, pregnant women) in relation to rubella virus antigen. Specific antihemagglutinins were found in 82%-93% of pregnant women, depending on their age. Examination of sera from 207 pregnant women who had contacts with rubella patients demonstrated clinically manifest rubella verified serologically in 9.2%, and asymptomatic form of rubella in 8.6%. By an immunochemical method (treatment of sera with staphylococca reagent) specific rubella IgM were found in 7 (100%) pregnant women who had experienced clinically manifest rubella, and in 6 (14.3%) pregnant women with asymptomatic rubella.
Subject(s)
Pregnancy Complications, Infectious/immunology , Rubella/immunology , Adolescent , Adult , Aged , Antigens, Viral/analysis , Child , Child, Preschool , Female , Humans , Immunoglobulin M/analysis , Immunohistochemistry , Infant , Middle Aged , Moscow , Pregnancy , Pregnancy Complications, Infectious/epidemiology , Rubella/epidemiologyABSTRACT
A number of two-phase aqueous polymers based on dextran (mol. w. 500) and polyethylene glycol (mol. w. 1000, 4000, 6000) was studied. The two-step distribution of virus-containing tissue culture fluid in these systems was found to result in significant purification of measles virus from protein admixtures. The degree of virus purification was shown to depend on ratios of volumes and molecular weights of the polymers.
Subject(s)
Measles virus/isolation & purification , Polymers/pharmacology , Dextrans/pharmacology , Methods , Molecular Weight , Polyethylene Glycols/pharmacology , Solubility , Viral Proteins/isolation & purification , Virus CultivationABSTRACT
The suspension of staphylocci containing protein A in their cell walls (strain Cowan 1) has been used for the determination of the immunological class of measles antibodies in the blood of measles patients and healthy persons. Adsorption with staphylococcal reagent is a simple and precise method for the detection of IgM which cannot be detected at equal or higher IgG titers when the samples under study are treated with reducing substances similar to mercaptoethanol. The advantages given by the use of protein A preparations for differentiating the nature of serum antibodies are shown, which is essential for the diagnosis and epidemiological analysis of infectious diseases.
