A red orange extract modulates the vascular response to a recreational dive: a pilot study on the effect of anthocyanins on the physiological consequences of scuba diving.

Nutritional antioxidants have been proposed as an expedient strategy to counter the potentially deleterious effects of scuba diving on endothelial function, flow-mediated dilation (FMD) and heart function. Sixteen volunteers performing a single standard dive (20 min at 33 m) according to US Navy diving procedures were randomly assigned to two groups: one was administered with two doses of 200 mg of an anthocyanins (AC)-rich extract from red oranges, 12 and 4 h before diving. Anthocyanins supplementation significantly modulated the effects of diving on haematocrit, body water distribution and FMD. AC administration significantly reduces the potentially harmful endothelial effects of a recreational single dive. The lack of any significant effect on the most common markers of plasma antioxidant capacity suggests that the mechanism underlying this protective activity is independent of the putative antioxidant effect of AC and possibly involves cellular signalling modulation of the response to high oxygen.

The effect of a passive stretch training protocol on performance during a drop jump in humans.

AIM: Our study's aim is to show how a five-week stretch training protocol, based on passive stretching, can change muscle performance during a drop jump (stretch shortening cycle). METHODS: This study observes in 8 healthy subjects (four males and four females), the effect of a 5-week passive stretch training protocol on the stretch shortening cycle (SSC) during the performance of a drop jump, and identify the architectural changes in the muscle. Subjects underwent measurements of their drop jump performance 3 times before, and 3 times after, the stretch training protocol. For the muscle tendon unit (MTU), changes were measured using the Hawkins and Hull's model. In order to calculate the length changes in the MTU, we measured the ankle and knee angles. For changes in the fascicle, the pennation angle and the thickness of the gastrocnemius medialis (GM) muscle were measured. RESULTS: After the 5-week stretch training protocol, a significant increase in flexibility was observed in the fascicle during the first phase of the jump (a shortening of 10% after training vs. 20% before). No significant change was observed in the tendon, while the MTU showed a highly significant improvement (P<0.01) in muscle compliance during the landing phase. The average performance during the drop jump also showed a highly significant change (P<0.01). CONCLUSION: The results show a higher degree of flexibility in the MTU, enabled the latter to store more energy which was then converted to kinetic energy during the push-off phase of the drop jump.

Are there hemodynamic implications related to an axillary arch?

The axillary arch (AA) has been thoroughly studied and described as a supernumerary muscle, present unilaterally or bilaterally. This study aims to provide an in vivo demonstration of the influence of an AA on vascular, biometrical, and hemodynamic parameters. Two-hundred thirty-nine subjects with a mean age of 21.3 ± 2.7 years participated in this study. After visual screening by two independent experts, 20 subjects (8.4%) presented with an AA unilaterally (n = 12) or bilaterally (n = 8). An echo-Doppler examination of the vena and arteria axillaris was performed to measure blood flow, velocity of circulating elements, and blood vessel diameter in different positions of the arm (abduction: 45°, 90°, 90° combined with exorotation 'ER', 120°). The arteria axillaris parameters, measured in the test (n = 9; six women, three men) and control group (n = 11; six women, five men), were equivalent for all tested positions. The axillary vein parameters, compared to variations within the groups, revealed no significant differences. However, when comparing variation between groups, significant differences were found for (i) diameter in 90° abduction + ER and 120° abduction; (ii) velocity in 90° and 120° abduction. Blood flow demonstrated no significant difference between groups in any of the positions. The results of this study indicate that there is no functional vascular implication of the AA in the test samples. This study also questions the interpretation of some hypotheses regarding the AA and entrapment syndromes.

Axillary web syndrome: nature and localization.

Axillary Web Syndrome (AWS) is a complication that can arise in patients following treatment for breast cancer. It is also known variously as syndrome of the axillary cords, syndrome of the axillary adhesion, and cording lymphedema. The exact origin, presentation, course, and treatment of AWS is still largely undefined. Because so little is known about AWS, we undertook a case series study consisting of 15 women who had undergone breast cancer surgery and presented with AWS. All subjects received a clinical examination which included body size determination and detailed measurements of the size and location of the cords. The cords were found to originate from the axilla, continue on the medial aspect of the arm up to the epitrochlea region, then to the anteromedian aspect of the forearm, and finally reaching the base of the thumb. The cords averaged approximately 44% of the limb length. Correlation of the cord location with anatomical studies shows that in fact this path follows the specific course taken by the antero-radial pedicle which arises at the anterior aspect of the elbow from the brachial medial pedicule to anastomose in the axilla at the level of the lateral thoracic chain nodes. Although our series is small, the correspondence between the physical findings and the anatomical studies strongly supports the notion that the cords are lymphatic in origin.

The role of the axillary arch (of Langer) in the management and the kinesiology of the overhead shoulder mobility.

AIM: Based on cadaveric, neurosurgical and medico-diagnostic evidence the axillary arch of Langer (AA) is assumed to create symptoms similar to those of entrapment or obstruction type syndromes, e.g. Thoracic Outlet Syndrome. Although the incidence of a AA varies between studies and races, there are many assumptions that its frequency and its axillary location influences the motor control of the shoulder girdle. In addition to the existing anatomical evidence and based on functional reasoning it can be predicted that the AA influences the shoulder girdle kinesiology in vivo also. However no study is known that verifies these functional assumptions in vivo. METHODS: In order to complete our knowledge of the AA we evaluated strength, endurance, motor control, precision and proprioception in vivo, e.g. hand held dynamometry, abduction and adduction shoulder strength and endurance, functional exercise assessment, throwing impact force on a (precision) target and shoulder joint position sense were measured in two groups of athletes and physical education students, one with an AA (N.=22) and a control group without AA (N.=22). RESULTS: The results indicate a significant (p<0.05) influence of the presence of an AA on strength, endurance and motor control increase in women associated with an increase of paraesthetics. For all these parameters no significant difference occurred in men. The throwing and proprioceptive joint position sense data however indicate a clear (P<0.05) increase of impact forces suggesting a possible shoulder stabilisation and an improvement of proprioception both in men and most in women. CONCLUSION: These finding have both a functional and clinical relevance and do not fully confirm the anatomical predictions from the cadaveric evidence nor support the (surgical) diagnoses of excision of the AA of Langer in throwing in athletes.

Efectos de un programa de entrenamiento estructurado de Gimnasia Abdominal Hipopresiva sobre la estática vertebral cervical y dorsolumbar / Effects of a program of constructed training in Hipopressive Gymnastic on the vertebral cervical and dorsolumbar statics

Objetivo. Mostrar los efectos de un entrenamiento de Gimnasia Abdominal Hipopresiva (1sesión semanal/10 semanas), sobre la estática de la columna dorsolumbar. Material. Se utilizaron como instrumentos de medición: plomada, cinta métrica; así como una cámara fotográfica Nikon Coolpix 5200, cuyas imágenes fueron tratadas por ordenador (software "Image Tool"). Métodos. Estudio simple ciego cuya población inicial fue de 29 estudiantes divididos en 2 grupos: al primero se le realizó un entrenamiento de 1 hora semanal durante 10 semanas de gimnasia abdominal hipopresiva, con estandarización de los ejercicios; y el segundo siguió sólo un entrenamiento postural. A todos se les realizó una serie de tests previos y posteriores al estudio: de flexibilidad de la columna vertebral, de extensibilidad muscular y mediciones posturales estáticas y dinámicas; de forma que cada parámetro fue comparado estadísticamente mediante el test Student-Newman-Keuls. Resultados. Hemos encontrado variaciones significativas en el grupo de estudio con respecto a los controles: en la estática vertebral (flecha cervical: p = 0,002; dorsal p = 0,0001; lumbar p = 0,006) en la posición del eje occipital (p = 0,04); y en las mediciones de flexibilidad de la columna, sobre todo en la flexión de tronco (p = 0,005). También hay un aumento de la fuerza isométrica de los músculos extensores del tronco: test de Sorensen-Biering (p = 0,02). Conclusiones. La gimnasia abdominal hipopresiva tiene incidencia en la estática de la columna dorsolumbar y creemos que estos resultados son muy alentadores y deben ser considerados; pero antes de poder ser aplicada con éxito en la reeducación dorsolumbar, debe proseguirse esta vía de investigación

Objective. Try to target the effects of a training of Gymnastic Hipopressive (GH) of 10 meetings on the statics of the dorsolumbar column. Material. Different instruments of measurement were used: plummet, metric tape; as well as a camera Nikon Coolpix 5200, which images were treated with the computer software "Image Tool". Methods. A double blind study, where the population were of the same age. Twenty nine students, divided in 2 groups: the first group was trained with GH for an hour a week during ten weeks, with standardization of the exercises; while the second one followed only a postural training. To all of them a series of test were realized before the study and also later: flexibility of the vertebral column, muscular extensibility and enclosed pose measurements static and dynamic; so that every parameter was compared statistically by means of the test Student-Newman-Keuls. Results. We have found significant variations in the group of study with regard to the controls: in the static of the spine (cervical arrow: p = 0.002; dorsal p = 0.0001; lumbar p = 0.006) in the position of the occipital axis (p = 0.04); as well as also in the measurements of flexibility of the column, above all, in the flexion of trunk parameters (p = 0.005). And also in the isometric strength of the expanding muscles: Sorensen-Biering test (p = 0.02). Conclusions. The GH has an effect on the statics of the dorsolumbar column and believe that these results are very encouraging and must be considered, nevertheless, the research must continue

Pulpal and periodontal temperature rise during KTP laser use as a root planing complement in vitro.

OBJECTIVE: The purpose of this study was to define the optimal irradiation conditions of a KTP laser during root planing treatment. METHODS: The surfaces of 60 single-root human teeth were scaled with conventional instruments before lasing. The pulpal temperature increase was measured by means of one thermocouple placed in the pulp chamber and a second one placed on the root surface at 1 mm from the irradiation site. The influence of variables of coloration by Acid Red 52 (photosensitizer), scanning speed, dentin thickness, and probe position was analyzed for a constant exposure time of 15 sec and 500 mw (spot size diameter, 0.5 mm). The pulpal temperature was below 3 degrees C for the adjustments. RESULTS: The irradiation on one point of root surface had the following results: The application of photosensitizer on the root surface before lasing produced a 50% higher temperature rise within the pulp than in the case without the application of the photosensitizer. The temperature rise in the pulp chamber was below 3 degrees C with the following settings of 500 mw: PW = 10 msec and PRR < 35; or PW= 20 msec and PRR < 20 Hz. On the other hand, for the same irradiation conditions, the temperature rise on the surface of the root was always below 7 degrees C. However, the temperature increase became higher than 7 degrees C (on the surface of the root) in the case of P > 500 mw, PW > 50 msec and PRR > 10 Hz of root surface or a scanning speed of irradiation of 1 mm/sec for a linear irradiation of 4 mm. CONCLUSION: The KTP laser may be used safely without thermal damage to pulp and periodontal tissue with respect to the biologically acceptable previously described parameters.

Surface-enhanced Raman scattering and surface-enhanced resonance Raman scattering excitation profiles of Ag-2,2'-bipyridine surface complexes and of [Ru(bpy)3]2+ on Ag colloidal surfaces: manifestations of the charge-transfer resonance contributions to the overall surface enhancement of Raman scattering.

Excitation profiles of SERS (surface-enhanced Raman scattering) and/or SERRS (surface-enhanced resonance Raman scattering) spectral bands of two forms of a Ag-bpy (bpy = 2,2'-bipyridine) surface complex and of [Ru(bpy)3]2+ on Ag nanoparticle (hydrosol) surfaces were determined from the spectra excited in the 458-600 nm region and are reported together with the FT-SERS spectra of the Ag-bpy surface complex and FT Raman spectra of [Ru(bpy)3] Cl2. Seven of the observed 11 fundamentals as well as their first overtones and combination bands are selectively enhanced in SERS of the Ag-bpy surface complex formed in the Ag colloid/HCl/bpy system. The profiles of these bands show a common maximum at approximately 540 nm. The selectively enhanced bands of the Ag-bpy surface complex have nearly the same wavenumbers as those enhanced in the SERRS and resonance Raman spectra of [Ru(bpy)3]2+ upon excitation close to the 453 nm maximum of its MLCT absorption band. Moreover, the intensity patterns of the bpy vibrations of the two species match both in resonance (541 nm excitation for Ag-bpy, 458 nm for [Ru(bpy)3]2+) and in off-resonance (458 and 1064 nm for Ag-bpy, 1064 nm for [Ru(bpy)3]2+). The distinct band shapes of the excitation profiles of the selectively enhanced vibrational modes of the Ag-bpy surface complex, as well as the observation of overtones and combination bands in the SERS spectra upon excitation into this "band", are interpreted in terms of a charge-transfer resonance contribution to the overall SERS enhancement. In view of the near-coincidence of the vibrational modes coupled to the resonant electronic transition of Ag-bpy with those coupled to the MLCT transition of [Ru(bpy)3]2+, the resonant electronic transition is tentatively assigned to a Ag metal to bpy (pi*) CT transition.

Raman and infrared studies of homogeneous forms of acid phosphatase from rat liver.

Raman spectra of acid phosphatase (orthophosphoric-monoester phosphohydrolase (acid optimum), EC 3.1.3.2) forms from rat liver in water solution, and infrared spectra of the same forms as thin films, have been investigated. The spectra show strong bands belonging to phosphodiester or phosphomonoester residues. These groups are modified during the postsynthetic modification of acid phosphatase and are probably connected with the process of bonding and splitting of mannose 6-phosphate and N-acetylglucosamine, in agreement with previous biochemical models for the intracellular transport of newly synthesized lysosomal hydrolases to lysosomes. Some other bands in the infrared spectra are assigned to vibrations of N-H groups which may belong to N-acetylglucosamine.