ABSTRACT
Silicon (Si) has been shown to suppress diseases of rice such as blast when applied to Si-deficient soils. In 1995 and 1996, Si was applied at 1,000 kg ha-1 to at two locations in eastern Colombia to determine if Si combined with reduced rates of fungicide could be used to manage leaf and neck blast effectively in upland rice. Two applications of edifenphos and three applications of tricyclazole were made at 0, 10, 25, or 100% of their labeled rates after amendment of soil with Si. At both locations, Si alone and Si combined with edifenphos reduced leaf blast severity by 22 to 75% when compared with nonamended, untreated controls, and suppression of leaf blast by Si alone was equal to or better than the full rate of edifenphos alone. Si alone suppressed neck blast as effectively or better than the full rate tricyclazole when severity was low; however, when severity was higher, a 10% rate of tricyclazole was needed in addition to Si. No differences in yield were observed between Si without fungicides and fungicides (full rate) without Si. Applications of Si made in 1995 had significant residual activity in terms of disease control and yield in 1996. The application of Si to Si-deficient soils may permit the use of reduced rates of fungicide to manage leaf and neck blast in upland rice.
ABSTRACT
Blast-resistant, partially resistant, and susceptible cultivars of rice were planted in soil amended with Si at 0, 500, or 1,000 kg/ha at two locations in eastern Colombia to assess differential responses to leaf blast, neck blast, and leaf scald, and to examine the quantity and quality of grains harvested. Leaf and neck blast on partially resistant and susceptible cultivars were reduced by Si as the rate of Si was increased. Depending on the location, the level of severity of leaf and neck blast on partially resistant cultivars, when fertilized with Si at 500 or 1,000 kg/ha, was lowered to that of resistant cultivars without Si. At both locations, yields were increased by as much as 42%, depending on the cultivar, by Si applied at 1,000 kg/ha. In general, high rates of Si reduced the number of broken grains harvested. Grain discoloration, regardless of cultivar or location, was reduced by as much as 70% at the high rate of Si. The application of Si to complement host resistance to blast and scald appears to be an effective strategy for disease management in rice and provides the added benefit of improving the quantity and quality of rice yields.
ABSTRACT
The latest wave of hospital reengineering has been based on the concepts of patient-focused care (PFC). PFC concepts include decentralizing low-scale activities to the point-of-care, cross-training employees to minimize hand-offs, simplifying processes to eliminate unnecessary complexity, redesigning the organization structure to focus on the care continuum, and adopting empowerment and teamwork to change individual behavior. Three innovative health systems have begun to reap the benefits of their PFC implementation efforts. Based on these three case study organizations, PFC is not only cost-justified, but also drives increases in quality and service levels. Each of the three case study organizations chose significantly different routes to implement these concepts due to various internal and external factors. Size of cost savings was the result of three main factors: level of change implemented, size of investment required to effect change, and time frame for implementation. Article includes results of economic analysis.
Subject(s)
Hospital Restructuring/economics , Patient-Centered Care/economics , Psychology, Industrial , Attitude of Health Personnel , California , Catholicism , Cost Savings/methods , Cost Savings/statistics & numerical data , Data Collection , Hospital Bed Capacity, 100 to 299 , Hospitals, Religious/economics , Hospitals, Religious/organization & administration , Leadership , Models, Organizational , Patient Satisfaction , TexasABSTRACT
Apamin and sarafotoxin are small peptide toxins which are 18 and 21 residues long, respectively. They both have cysteines at positions 1, 3, 11, and 15. However, the non-cysteine portions of their sequences and the positions of their disulfides are different. In native apamin, the cysteines form disulfides 1-11 and 3-15, whereas in sarafotoxin they form the 1-15 and 3-11 pairs. Truncated analogs have been synthesized which lack the carboxyl-terminal tails following cysteine-15. When oxidized by glutathione, both truncated sequences retain the ability to selectively populate the disulfide combination observed in the respective full-length parent. This ability is retained in the presence of the denaturing agent 5 M guanidinium chloride. Circular dichroism spectra of the nativelike isomers are nearly identical to those of the parent sequence, and are not affected by heating to 75 degrees C or exposure to 5 M guanidinium chloride. The alpha helix observed in apamin is a consequence of both the disulfide topology and the non-cysteine portions of the sequence. There is not much alpha helix when apamin is forced to adopt the disulfides found in native sarafotoxin or when sarafotoxin is forced to adopt the disulfides found in native apamin.
Subject(s)
Apamin/metabolism , Disulfides/metabolism , Viper Venoms/metabolism , Amino Acid Sequence , Apamin/chemistry , Circular Dichroism , Glutathione/metabolism , Glutathione/pharmacology , Guanidine , Guanidines/pharmacology , Hot Temperature , Molecular Sequence Data , Oxidation-Reduction , Protein Structure, Secondary , Viper Venoms/chemistryABSTRACT
This study was conducted to determine the degradation rates in turfgrass soil over a 12-month period after a single field application of propoxur and to isolate microorganisms from the soil capable of degrading the insecticide. Soil samples were collected from a turfgrass experimental site near Fort Lauderdale, FL one week before the field application of propoxur, and over a 12-month period after the field application. Mineralization rates in surface (0-15 cm depth) and subsurface (15-30 cm depth) soil samples collected before the field application were low. Mineralization in surface and subsurface samples collected 1, 6 and 8 months after the field application was much higher than for corresponding samples collected before the field application. Mineralization in the subsurface samples collected 12 months after the field application had reverted back to the similar rate for the corresponding sample collected before field application. Half-life values (t1/2) for propoxur showed similar trends to the results of mineralization. After a single application of propoxur, degradation in turfgrass soil was enhanced. Such enhancement lasted less than 12 months for the subsurface, but more than 12 months for the surface. A strain of Arthrobacter sp. capable of degrading propoxur was isolated from the soil.
Subject(s)
Arthrobacter/metabolism , Propoxur/metabolism , Soil Microbiology , Arthrobacter/isolation & purification , Biodegradation, Environmental , Time FactorsABSTRACT
alpha-Conotoxin GI is a snail toxin protein consisting of 13 amino acids cross-linked by 2 intramolecular disulfide bridges. This toxin is an antagonist of acetylcholine receptors. The native sequence has been synthesized, along with nine additional variants in which non-cysteine residues are replaced by alanine or the cysteine positions are altered. Each reduced peptide has been oxidized by reaction with oxygen or glutathione both in a folding buffer and in 6 M guanidine hydrochloride. Purified products of oxidation have been characterized with respect to molecular weights and the positions of disulfides. The four cysteines in conotoxin can form two intramolecular disulfides in three different combinations. Relative yields of each of the three isomers have been determined, thereby permitting evaluation of the roles of non-cysteine residues and cysteine placements in the folding of conotoxin. Cysteine positions dominate factors directing formation of the nativelike isomer in a manner that may be predicted from equilibrium constants for loop formation in model peptides containing two cysteines. Alanine substitutions at several positions which are conserved in naturally occurring conotoxins affect the discrimination between the two most favored disulfide arrangements. Substitutions at three nonconserved positions have no structural effect on isomer yields. It therefore is possible to vary these latter three positions in a manner which might help to generate a functional binding surface which is complementary to receptors in the specific prey of a particular species of snail, without affecting the toxin's folding.
Subject(s)
Conotoxins , Mollusk Venoms/chemistry , Peptides, Cyclic/chemistry , Amino Acid Sequence , Disulfides/analysis , Glutathione , Guanidine , Guanidines/pharmacology , Kinetics , Molecular Sequence Data , Oxidation-Reduction , Peptides/chemical synthesis , Peptides/chemistry , Peptides, Cyclic/chemical synthesis , Protein ConformationABSTRACT
Microscopic disulfide-exchange rate constants have been measured for the formation and opening of small disulfide loops in reactions between glutathione and peptides containing 2 cysteines. Twelve cysteine-Xm-cysteine peptides have been studied, where X is an amino acid and m is the number of amino acids between the cysteines. Homopolymers of alanine for m equaling 0-5 are evaluated, as well as X1 and X2 series employing glycine, valine, or proline. Equilibrium constants Kc for loop closing are only slightly dependent on the nature of X. Loops with even values of m generally are favored relative to loops with odd values. Kc increases in the rank order X1, X3, X0, X5, X4, and X2. Formation of a disulfide between sequentially adjacent cysteines therefore is not especially difficult. The dependence of Kc on the odd-even nature of m is compared with similar patterns observed both in statistics of disulfide formation in naturally occurring proteins and in theoretical studies of peptide cyclization. The relative equilibrium populations of intramolecular disulfides in peptides containing cysteine-cysteine-cysteine and cysteine-serine-cysteine-serine-cysteine clusters are consistent with predictions based on the values of Kc in the two-cysteine peptides.
Subject(s)
Cysteine , Disulfides , Peptides , Amino Acid Sequence , Chemical Phenomena , Chemistry, Physical , Chromatography, High Pressure Liquid , Kinetics , Molecular Sequence Data , Protein ConformationABSTRACT
Rate constants have been determined in 3 M guanidine hydrochloride for disulfide exchange reactions between glutathione and two synthetic peptides containing a cysteine-valine-cysteine region. Equilibrium experiments demonstrate the absence of noncovalent peptide aggregation in this solvent. Procedures are given for separating seven different components in quenched reactions, including the fully reduced cysteine cluster, the monomeric disulfide loop, parallel and antiparallel dimer loops, and the three monomers containing one or two mixed disulfides with glutathione. Intramolecular rate constants for (1) formation of a sterically strained monomer loop, (2) transfer of glutathione between the two cysteines on the same peptide chain, and (3) formation of unstrained dimer loops correspond to a series of processes forming rings of increasing size. In one sequence, these rate constants are 3, 6, and about 21 s-1, respectively. The larger loops are formed more easily. In the other sequence, rate constants for formation and opening of monomer loops are accelerated 180- and 1300-fold, respectively, relative to analogous reactions in a peptide containing eight residues between the two cysteines. This gives a 7-fold smaller equilibrium constant for ring closure in the cysteine cluster. Dimer formation occurs by a mechanism utilizing the accelerated opening of monomer loops. Results provide information assisting efforts to develop strategies for directing disulfide pairing in novel protein structures. Results also help define factors contributing to formation of undesired oligomers during efforts to refold cysteine-containing proteins obtained by bacterial expression of mammalian genes.
Subject(s)
Oligopeptides , Chromatography, High Pressure Liquid , Cysteine , Disulfides , Glutathione , Indicators and Reagents , Kinetics , Oligopeptides/chemical synthesis , Oxidation-Reduction , Protein Conformation , ValineABSTRACT
The cyanogen bromide fragment comprising residues 115-181 of Kunitz soybean trypsin inhibitor is a soluble random-coil peptide at pH 7 containing two cysteines separated by eight other amino acids in the primary sequence. Four of the six rate constants have been determined for the three disulfide exchange reactions between this fragment and oxidized and reduced forms of N-acetylcysteine methyl ester. The rate constant for intramolecular loop formation in the fragment containing one thiolate anion and one sulfur connected by a disulfide bond to the small cysteine analogue is 0.36 +/- 0.15 s-1 at 23 degrees C in 3 M guanidine hydrochloride. This measurement provides a frame of reference corresponding to formation of a small but sterically unstrained loop, the fast limit for intramolecular disulfide exchange in a random-coil peptide.
Subject(s)
Cysteine , Peptide Fragments , Protein Conformation , Trypsin Inhibitor, Kunitz Soybean , Trypsin Inhibitors , Cyanogen Bromide , Disulfides , Dithiothreitol , Glutathione , Guanidine , Guanidines , Iodoacetates , Kinetics , Oxidation-Reduction , UreaABSTRACT
Acid dissociation equilibrium constants and rate constants for disulfide interchange reactions have been measured in 50% methanol at low ionic strength for peptides containing cysteines with local ionic neighboring groups. These physical constants may be correlated by separation of free energy contributions into solvent-independent and solvent-dependent factors. The former represent inductive effects which may be evaluated by extrapolation of pKa values to the limit of infinite ionic strength. These solvent-independent contributions give Br onsted coefficients consistent with previously reported values for disulfides with neutral constituents. The solvent-dependent contributions represent thru-solvent electrostatic effects and are consistent with the form of the Bjerrum relationship correlating molecular charges, intergroup distances, and the dielectric constant of the solvent. These results provide a quantitative framework for developing strategies for employing coulombic interactions to direct disulfide pairing in synthetic polypeptides.
Subject(s)
Disulfides/metabolism , Sulfhydryl Compounds/metabolism , Energy Transfer , Mathematics , Methanol , Models, Chemical , Osmolar ConcentrationABSTRACT
The role of electrostatic factors has been evaluated for the reversible disulfide exchange reaction between N-acetylcysteine (A) and a peptide fragment (B) comprising residues 85-114 of Kunitz soybean trypsin inhibitor. In A, the sulfhydryl group has a negative carboxyl neighbor on the cysteine itself. In B, the only charged group within five residues of the single cysteine at position 86 is the positive N-terminal amino group on residue 85. The concentrations of the monomers A and B and of the disulfides AA, AB and BB have been determined as a function of time in kinetic experiments at pH 7, 23 degrees C and ionic strengths of 20 mM and 1 M. At both ionic strengths the sulfhydryl acid dissociation constants Ka have been determined for A and B, as well as the four rate constants for the disulfide exchange reaction. The electrostatic effects are small in magnitude but occur in expected directions. Local cysteine environments enhance formation of the mixed disulfide (AB), having a favorable configuration of adjacent unlike charges and generate decreases in the AA and BB disulfides joining regions of identical charge. These experiments represent an initial step towards use of intrinsic protein functional groups to direct formation of specific disulfides in a synthetic protein.
Subject(s)
Acetylcysteine , Trypsin Inhibitor, Kunitz Soybean , Trypsin Inhibitors , Cysteine , Disulfides , Electrochemistry , Kinetics , Osmolar Concentration , Peptide Fragments , Structure-Activity Relationship , Sulfhydryl ReagentsABSTRACT
The ionic strength dependence of the bimolecular rate constant for reaction of the negative disulfide 5,5'-dithiobis (2-nitrobenzoic acid) with cysteines in fragments of naturally occurring proteins was determined by stopped-flow spectroscopy. The Debye-Hückel relationship was applied to determine the effective charge at the cysteine and thereby determine the extent to which nearby neighbors in the primary sequence influence the kinetics. Corrections for the secondary salt effect on cysteine pKs were determined by direct spectrometric pH titration of sulfhydryl groups or by observation of the ionic strength dependence of kinetics of cysteine reaction with the neutral disulfide 2,2'-dithiodipyridine. Quantitative expressions was verified by model studies with N-acetyl-cystein. At ionic strengths equal to or greater than 20 mM, the net charge at the polypeptide cysteine site is the sum of the single negative charge of the thiolate anion and the charges of the amino acids immediately preceding and following the cysteine in the primary sequence. At lower ionic strengths, more distant residues influence kinetics. At pH 7.0, 23 degree C, and an ionic strength of 20 mM, rate constants for reaction of the negative disulfide with a cysteine having two positive neighbors, one positive and one neutral neighbor, or two neutral neighbors are 132000, 3350, and 367 s-1 M-1, respectively. This corresponds to a contribution to the activation energy of 0.65- 1.1 kcal/mol per ion pair involved in collision between the cysteine and disulfide regions. The results permit the estimation that cysteine local environments may provide a means of achieving a 10(6)-fold range in rate constants in disulfide exchange reactions in random-coil proteins. This range may prove useful in developing strategies for directing disulfide pairing in synthetic proteins.
Subject(s)
Cysteine , Dithionitrobenzoic Acid , Nitrobenzoates , Proteins , Cyanogen Bromide , Hydrogen-Ion Concentration , Kinetics , Mathematics , Osmolar Concentration , Pepsin A , Peptide Fragments , Protein BindingABSTRACT
Proton nuclear magnetic resonance (NMR) spin lattice relaxation time (T1) and spin-spin relaxation time (T2) measurements are presented for a number of proteins with molecular weights spanning the range of 6,500-150,000 daltons. These measurements provide experimental evidence for the role of cross-relaxation in 1H NMR T1 measurements in proteins. The relationship between these measurements and the theory recently presented by Kalk and Berendsen is discussed. The results indicate that cross-relaxation dominates the T1 measurements for the larger proteins, even at relatively low resonance frequencies such as 100 MHz.
Subject(s)
Proteins , Chemical Phenomena , Chemistry, Physical , Magnetic Resonance Spectroscopy , Molecular Weight , Motion , ProtonsABSTRACT
The interactions of Pr(III) and Eu(III) with specifically nitrated derivatives of the basic bovine pancreatic trypsin inhibitor have been studied using optical spectroscopy and nuclear magnetic resonance (NMR) at 250 and 270 MHz. Stability constants for proton and metal binding to nitrotyrosines 10 and 21 determined optically are in good agreement with those from NMR. Observations of the Eu(III)-induced NMR shifts of the ring protons of nitrotyrosine 21 allowed calibration of the magnetic interactions for this binding site. The Pr(III)-induced shifts for several resolved nonexchangeable backbone proton resonances were compared with calculated shifts using the known x-ray structure. With several simplifying assumptions, the Pr(III)-induced shifts were used to assign one alpha-CH and five NH protons to compatible sets of backbone positions which are consistent with the known pH dependence and resistance to exchange with solvent D2O. Some of the more general aspects of lanthanide-induced shifts are discussed with reference to their use in proteins. Due to the complexities of the analysis of the shift data, the most straightforward use of this technique is in conjunction with the relaxation probe Gd(III) for measurement of intramolecular distances.
Subject(s)
Trypsin Inhibitor, Kazal Pancreatic , Trypsin Inhibitors , Tyrosine/analogs & derivatives , Animals , Binding Sites , Cattle , Kinetics , Magnetic Resonance Spectroscopy , Mathematics , Protein Binding , Protein Conformation , Structure-Activity RelationshipABSTRACT
Nitration of tyrosine has been investigated as a means for chemically introducing lanthanide chelating sites at known positions in proteins. The low-field portions of the 250-MHZ and 270-MHZ 1H nuclear magnetic resonance spectra of native and chemically modified bovine pancreatic trypsin inhibitor have been studied in the presence of lanthanide ions. Comparisons of spectral changes observed with native, mononitro (tryosine 10) and dinitro (tyrosines 10 and 21) derivatives enable these changes to be separately attributed to metal bound at nitrotyrosine 21, nitrotyrosine 10, or the set of five carboxyl groups. The pH dependence of Pr(III) and Eu(III) induced chemical shifts yields stability constants of 50 and 159 M-1 for the association between lanthanides and nitrotyrosines 10 and 21, respectively. Correlation times for the interactions with Gd(III) bound to specific nitrotyrosines are estimated from the induced line broadening of resonances of the nitrotyrosine ring protons. These stability constants and correlation times are used to determine the distances from the different metal binding sites to buried backbone NH protons having resolved resonances. Comparisons with distances in the x-ray crystal structure give assignments of the NH resonances to a small set of buried backbone NH's.
Subject(s)
Metals, Rare Earth , Trypsin Inhibitor, Kazal Pancreatic , Trypsin Inhibitors , Tyrosine/analogs & derivatives , Animals , Binding Sites , Cattle , Hydrogen-Ion Concentration , Kinetics , Magnetic Resonance Spectroscopy , Mathematics , Protein Binding , Protein ConformationABSTRACT
The low-field portions of the 250-MHz 1H nuclear magnetic resonance spectra of native and chemically modified basic pancreatic trypsin inhibitor have been studied as a function of pH over the range pH 5-13. In derivatives selectively reduced and carboxamidomethylated at cystine 14-38, resonances associated with 15 of the 16 protons of the aromatic rings of the four tyrosines of the inhibitor have been located and assigned to specific tyrosyl residues. Titrations of pH yielded pK's for tyrosines 10, 21, 23, and 35 in the modified inhibitor of 9.9, 10.6, 11.6, and 11.0, respectively. Resonances associated with the three nitrotyrosine 10 protons of the mononitrated derivative and the six nitrotyrosine 10 and 21 protons of the dinitrated derivative have been similarly located, assigned, and titrated, yielding pK's for nitrotyrosines 10 and 21 of 6.5 and 6.4, respectively. Previously reported results for derivatives with cystine 14-38 intact have been revised on the basis of new data. Comparison of these revised results with the new data for derivatives with modified cystine 14-38 reveals no changes in pK's for any tyrosine or nitrotyrosing ring and no changes in chemical shift for resonances of nitrotyrosine 21 or tyrosines 21 and 23. However, modification of cystine 14-38 causes significant changes in chemical shifts of resonances of the nearby nitrotyrosine 10 and tyrosine 10 and 35 rings. Tyrosine 35 remains relatively immobile, rotating less than 1600 times/s at 25 degrees C for pH's in the range 5-13.
Subject(s)
Aprotinin , Animals , Binding Sites , Cattle , Cystine/analysis , Disulfides/analysis , Guanidines , Hydrogen-Ion Concentration , Iodoacetamide , Magnetic Resonance Spectroscopy , Protein Binding , Protein Conformation , Tyrosine/analysis , UreaABSTRACT
The low-field portions of the 250-MHz 1H nuclear magnetic resonance (NMR) specra of native and chemically modified bovine basic pancreatic trypsin inhibitor (BPTI) have been studied as a function of pH over the range pH 5-13. Resonances associated with the 16 protons of the aromatic rings of the four BPTI tyrosines have been located and assigned to specific tyrosyl residues. Titrations of pH yielded pK's for tyrosines-10, -21, -23, and -35 of 10.4, 11.0, 11.7, and 11.1, respectively. The resonances associated with the nitrotyrosine-10 protons of mononitrated BPTI and the nitrotyrosine-10 and -21 protons of dinitrated BPTI have been similarly located, assigned and titrated yielding pK's for nitrotyrosine-10 and -21 of 6.6 and 6.4, respectively. The high-field NMR spectrum indicates that the aromatic ring of tyrosine-35 rotates less than 160 times per second at 25 degrees for pH's in the range 5-9.