ABSTRACT
It has been hypothesized that the therapeutic effects of artepillin C, a natural compound derived from Brazilian green propolis, are likely related to its partition in the lipid bilayer component of biological membranes. To test this hypothesis, we investigated the effects of the major compound of green propolis, artepillin C, on model membranes (small and giant unilamelar vesicles) composed of ternary lipid mixtures containing cholesterol, which display liquid-ordered (lo) and liquid-disordered (ld) phase coexistence. Specifically, we explored potential changes in relevant membrane parameters upon addition of artepillin C presenting both neutral and deprotonated states by means of small angle X-ray scattering (SAXS), differential scanning calorimetry (DSC), and confocal and multiphoton excitation fluorescence microscopy. Thermotropic analysis obtained from DSC experiments indicated a loss in the lipid cooperativity of lo phase at equilibrium conditions, while at similar conditions spontaneous formation of unilamellar vesicles from SAXS experiments showed that deprotonated artepillin C preferentially located at the surface of the membrane. Time-resolved experiments using fluorescence microscopy showed that at doses above 100 µM, artepillin C in its neutral state interacted with both liquid-ordered and liquid-disordered phases, inducing curvature stress and promoting dehydration at the membrane interface.
Subject(s)
Lipid Bilayers/chemistry , Liposomes/chemistry , Phenylpropionates/chemistry , 2-Naphthylamine/analogs & derivatives , Calorimetry, Differential Scanning , Cholesterol/chemistry , Laurates , Microscopy, Confocal , Microscopy, Fluorescence , Models, Chemical , Reference Values , Reproducibility of Results , Scattering, Small Angle , Temperature , Time FactorsABSTRACT
It has been hypothesized that the therapeutic effects of artepillin C, a natural compound derived from Brazilian green propolis, are likely related to its partition in the lipid bilayer component of biological membranes. To test this hypothesis, we investigated the effects of the major compound of green propolis, artepillin C, on model membranes (small and giant unilamelar vesicles) composed of ternary lipid mixtures containing cholesterol, which display liquid-ordered (lo) and liquid-disordered (ld) phase coexistence. Specifically, we explored potential changes in relevant membrane parameters upon addition of artepillin C presenting both neutral and deprotonated states by means of small angle X-ray scattering (SAXS), differential scanning calorimetry (DSC), and confocal and multiphoton excitation fluorescence microscopy. Thermotropic analysis obtained from DSC experiments indicated a loss in the lipid cooperativity of lo phase at equilibrium conditions, while at similar conditions spontaneous formation of unilamellar vesicles from SAXS experiments showed that deprotonated artepillin C preferentially located at the surface of the membrane. Time-resolved experiments using fluorescence microscopy showed that at doses above 100 µM, artepillin C in its neutral state interacted with both liquid-ordered and liquid-disordered phases, inducing curvature stress and promoting dehydration at the membrane interface.
Subject(s)
Phenylpropionates/chemistry , Lipid Bilayers/chemistry , Liposomes/chemistry , Reference Values , Temperature , Time Factors , Calorimetry, Differential Scanning , Cholesterol/chemistry , Reproducibility of Results , Microscopy, Confocal , Scattering, Small Angle , Laurates , Microscopy, Fluorescence , Models, Chemical , 2-Naphthylamine/analogs & derivativesABSTRACT
Pollination services performed by bees are essential for the reproduction of a great part of flowering plants. The pollen collected by Apis mellifera while performing pollination (bee pollen), has been incorporated into the human diet for its favorable nutritional components. Around 1,500 tons of bee pollen are produced annually worldwide, especially in Spain, China, Australia, Argentina, and Brazil. Despite the importance of bee pollen within apiculture, little is known about the effects of climate variations on bee pollen collection and production. We monitored the pollen collection performance of 24 different honey bee colonies in different climate conditions within a period of one year. We then analyzed the statistical interaction among the number of worker bees returning with pollen loads and 12 climatic variables, to produce a predictive mixed linear model. The results obtained showed that 7 climatic variables were statistically correlated to the pollen collection observed: Maximum temperature of the day, minimum temperature of the day, dew point temperature, relative humidity, cloud cover, rainfall, and the date of the sample. This research brings information for the development of a more effective pollen productive system.(AU)
Serviços de polinização são essenciais para a reprodução de uma grande parte das plantas com flores. Além de fundamental para produtividade agrícola e segurança alimentar no planeta, a atividade de polinização por Apis mellifera também possibilita a produção do pólen apícola. Devido a sua riqueza nutricional, o pólen apícola vem sendo incorporado às dietas humanas e de animais de interesse zootécnico. Cerca de 1.500 toneladas de pólen de abelha são produzidas anualmente em todo o mundo, especialmente em países como Espanha, China, Austrália, Argentina e Brasil. Apesar da importância do pólen na cadeia produtiva apícola, muito pouco se conhece sobre os efeitos das variações climáticas sobre a coleta e a produtividade de pólen. Neste estudo, foi monitorado o desempenho da coleta de pólen de 24 colônias de abelhas africanizadas, em diferentes condições climáticas, por um período de um ano. Analisaram-se estatisticamente as interações dos dados obtidos para o número de abelhas operárias que retornaram com cargas de pólen nas corbículas e 12 variáveis climáticas, a fim de se produzir um modelo estatístico preditivo. Os resultados obtidos mostraram que sete variáveis climáticas influenciaram significativamente a coleta de pólen: temperatura máxima do dia, temperatura mínima do dia, temperatura do ponto de orvalho, umidade relativa, cobertura de nuvens, precipitação pluviométrica e data da amostragem. Esta pesquisa traz informações importantes para o desenvolvimento de um sistema produtivo de pólen apícola mais eficaz.(AU)
Subject(s)
Animals , Bees/growth & development , Climate Change/classification , Pollen/growth & development , PollinationABSTRACT
The Meliponini, also known as stingless bees, are distributed in tropical and subtropical areas of the world and plays an essential role in pollinating many wild plants and crops These bees can build nests in cavities of trees or walls, underground or in associations with ants or termites; interestingly, these nests are sometimes found in aggregations. In order to assess the genetic diversity and structure in aggregates of Nannotrigona testaceicornis (Lepeletier), samples of this species were collected from six aggregations and genetically analyzed for eight specific microsatellite loci. We observed in this analysis that the mean genetic diversity value among aggregations was 0.354, and the mean expected and observed heterozygosity values was 0.414 and 0.283, respectively. The statistically significant Fis value indicated an observed heterozygosity lower than the expected heterozygosity in all loci studied resulting in high homozygosis level in these populations. In addition, the low number of private alleles observed reinforces the absence of structuring that is seen in the aggregates. These results can provide relevant information about genetic diversity in aggregations of N. testaceicornis and contribute to the management and conservation of these bees' species that are critical for the pollination process.
Subject(s)
Bees/genetics , Genetic Variation , Animals , Brazil , Heterozygote , Microsatellite RepeatsABSTRACT
The use of bee-pollen as a nutritional supplement or as a production-enhancing agent in livestock has increased the demand for this product worldwide. Despite the current importance of this niche within the apiculture industry, few studies have addressed the pollen production. We tested the performance of free-mated (FM) and instrumentally inseminated queens (IQ) in order to establish the effect of different breeding systems on pollen production. The F1 generation of IQ queens produced 153.95±42.83g/day, showing a significant improvement on the pollen production (2.74 times) when compared to the parental generation (51.83±7.84g/day). The F1 generation of free-mated queens produced 100.07±8.23 g/day, which increased by 1.78 times when compared to the parental generation. Furthermore, we observed a statistically significant difference between the pollen production between colonies from the IQ and FM treatments. This study suggests that inseminated queens should be considered by beekeepers that aim to increase pollen production.(AU)
O uso do pólen apícola como suplemento nutricional humano e animal tem aumentado significativamente a demanda por este produto em todo o mundo. Apesar da importância atual desse nicho dentro da apicultura, poucos estudos têm abordado melhorias no sistema produtivo de pólen. Testamos o desempenho de rainhas fecundadas através de acasalamento livre (FM) e inseminação instrumental (QI), a fim de analisar o efeito de diferentes técnicas de melhoramento genético no ganho produtivo de pólen apícola. A geração F1 de rainhas QI produziu 153,95±42,83g de pólen por dia, mostrando uma melhora significativa na produção (2,74 vezes) quando comparado à geração parental (51,83±7,84g/dia). A geração F1 de rainhas de livre acasalamento produziu 100,07±8,23g/dia, o que significou um aumento de 1,78 vezes. Além disso, observou-se uma diferença estatisticamente significativa entre a produção de pólen entre as colônias dos tratamentos QI e FM. Este estudo sugere que a técnica de inseminação instrumental deve ser incorporada nos sistemas produtivos de pólen apícola brasileiro.(AU)
Subject(s)
Animals , Bees/growth & development , Insemination, Artificial , Pollen , BeekeepingABSTRACT
Queen, worker, and male production was studied for 1 year in three queenright colonies of Tetragonisca angustula (Latreille). We sampled brood combs monthly and noticed that the number of brood cells and production of individuals were similar among colonies. Although the production of queens did not vary significantly, the frequencies of workers, males, and the number of cells among the combs varied over time. The production of males was highly seasonal, occurring mostly from February to April, coinciding with the period of intense brood cells production, when colonies produced more males and less workers, resulting in a negative correlation. Although the frequency of queens has not varied in time, the seasonal availability of males affected the mating frequency and the time spent since emergence until fertilization of queens. In the T. angustula colonies studied, the population dynamics was highly seasonal and the mating success depended of male production, according to the season.
Subject(s)
Bees , Reproduction , Animals , Hymenoptera , Male , Population Dynamics , SeasonsABSTRACT
We examined the antibacterial activities of several types of propolis, including Africanized honey bee green propolis and propolis produced by meliponini bees. The antibacterial activity of green propolis against Micrococcus luteus and Staphylococcus aureus was superior to that of Melipona quadrifasciata and Scaptotrigona sp propolis. Only two samples of propolis (green propolis and Scaptotrigona sp propolis) were efficient against Escherichia coli. Melipona quadrifasciata propolis was better than green propolis and Scaptotrigona sp propolis against Pseudomonas aeruginosa. We concluded that these resins have potential for human and veterinary medicine.
Subject(s)
Anti-Bacterial Agents/pharmacology , Bacteria/drug effects , Bees/chemistry , Propolis/pharmacology , Animals , Escherichia coli/drug effects , Pseudomonas aeruginosa/drug effectsABSTRACT
Mitochondrial genotypes of Africanized honeybees from Brazil and Uruguay were surveyed by DraI restriction of the COI-COII region. Eleven mitotypes were found, three of which had not previously been described (A28-A30). Out of 775 samples (725 from Brazil, 50 from Uruguay), 197 were A1 and 520 were A4. A1 frequency increases toward the north of Brazil, whereas A4 frequency increases toward the south, a pattern echoing the African distribution. The origin of the A4 and most of the A1 African patterns can be attributed to the introduction of Apis mellifera scutellata into Brazil in 1956. The A29 and A30 patterns have the P1 sequence observed in many Iberian Peninsula samples, which represent the traces of the introductions into Brazil and Uruguay by settlers.
Subject(s)
Bees/enzymology , Bees/genetics , DNA, Mitochondrial/genetics , Electron Transport Complex IV/genetics , Africa , Animals , Base Sequence , Brazil , Gene Flow , Genetics, Population , Molecular Sequence Data , Polymorphism, Restriction Fragment Length , Sequence Homology, Nucleic Acid , UruguayABSTRACT
The pollen spectra of honey samples collected in five apiaries situated near cerrado areas in Minas Gerais State, Brazil, were studied from April 1996 to April 1998 in order to establish the contribution of different plant species in pollen or nectar production The honey samples were characterized by the following native species: Astronium sp., Alternanthera sp., Schinus sp., and Serjanea sp. The pollinic participation percentage of those species was related to the degree of preservation in the areas.
Subject(s)
Animals , Honey , Plants , Pollen , Bees , Brazil , SeasonsABSTRACT
The pollen spectra of honey samples collected in five apiaries situated near cerrado areas in Minas Gerais State, Brazil, were studied from April 1996 to April 1998 in order to establish the contribution of different plant species in pollen or nectar production The honey samples were characterized by the following native species: Astronium sp., Alternanthera sp., Schinus sp., and Serjanea sp. The pollinic participation percentage of those species was related to the degree of preservation in the areas.
Subject(s)
Honey/analysis , Plants/classification , Animals , Bees , Brazil , Pollen/classification , SeasonsABSTRACT
Neste trabalho é descrito o alto grau de polimorfismo da hexoquinase (HK) em abelhas africanizadas do Brasil e confirmado o monomorfismo deste sistema em abelhas de origem européia. A detecçäo da alozima 87 em abelhas africanizadas do Brasil é explicada pela introduçäo de Apis mellifera scutellata no país em 1956. A frequência do gene HK nas populaçöes brasileiras analisadas é maior em Luiz Antonio, Estado de Säo Paulo, localidade próxima a Rio Claro, centro inicial de dispersäo dos enxames africanos. A frequência deste alelo diminui à medida que populaçöes situadas a uma maior distância deste centro säo amostradas, particularmente, em áreas mais ao sul do país, onde a atividade apícola com base em abelhas européias foi mais intensa. A detecçäo deste polimorfismo se reveste de significativa importância para o rastreamento de genes africanos em populaçöes de Apis mellifera existentes no continente americano
