Potential use of a thermal water cyanobacterium as raw material to produce biodiesel and pigments.

Global energy demand is increasing every day and most is still derived from non-renewable sources. Therefore, sustainable alternatives are sought to produce biofuels, such as biodiesel. Several studies have demonstrated the potential of microalgae and cyanobacteria to produce biodiesel and pigments. These pigments, such as lutein and astaxanthin, have a high commercial value and can economically support the production of biodiesel. However, few studies have explored the potential of cyanobacteria collected in thermal water. In these microorganisms, both biomass and metabolites production can be altered by the culture form. Thus, a cosmopolitan filamentous cyanobacterium (Geitlerinema amphibium) from thermal water was collected and isolated to evaluate its potential to produce fatty acids, biodiesel, and pigments in two culture media. G. amphibium was cultured in WC (Wright's Cryptophyte) and BBM (Bold's Basal Medium) media. Thermal stress (40 °C for 48 h) was applied to the medium, which generated higher productivity of the biomass in BBM. The cyanobacterium contained higher biodiesel content in the WC medium and higher pigment content in the BBM medium. Thermal stress increased the biodiesel content by 350%, but decreased pigment content. Two pigments with high commercial value (astaxanthin and lutein) were identified. G. amphibium produced up to 2.74 mg g-1 of astaxanthin and 5.49 mg g-1 of lutein, which is seven times more lutein than Marigold, currently the main raw material used commercially. Therefore, G. amphibium has the potential to produce biodiesel, astaxanthin, and lutein concomitantly.

Analysis of major carotenoids and fatty acid composition of freshwater microalgae.

Considering the nutraceutical properties, the high commercial value from pigments and essential lipids and the environmental sustainability, the purposes of this study were to assess the major carotenoids and fatty acids composition of nine microalgae species as a source of nutraceutical compounds and as fatty raw material for biodiesel production. The carotenoid and fatty acid content were analyzed by high performance liquid chromatography tandem mass spectrometry detection method with atmospheric pressure chemical ionization mode (HPLC/APCI-MS/MS) and by high resolution gas chromatography with flame ionization detector (GC-FID). For the carotenoid analysis, the developed method presented a rapid response, a good chromatographic separation, higher sensitivity and can provides more compounds information due the mass spectrum. Among the microalgae evaluated, Desmodesmus protuberans (10.3 mg g-1), Desmodesmus denticulatus var. linearis (8.43 mg g-1) and Chlamydomonas planctogloea (7.4 mg g-1) are good lutein sources. Coelastrum sphaericum (15.29 mg g-1) and Parachlorella kessleri (22.96 mg g-1) showed high astaxanthin content; the others microalgae species presents low carotenoid content. In addition, Chlorella zofingiensis provides high quantities of γ-linolenic acid (4.3%). Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) levels were lower than 1.1 %. Regarding for biodiesel production, the promising strains are Coelastrum sphaericum and Parachlorella kessleri.