Diagnostic ultrasound assessment of deep fascia sliding mobility in vivo: A scoping review - Part 2: Femoral and crural fasciae.

BACKGROUND: Failure of fascial sliding may occur in cases of excessive or inappropriate use, trauma, or surgery, resulting in local inflammation, pain, sensitization, and potential dysfunction. Therefore, the mechanical properties of fascial tissues, including their mobility, have been evaluated in vivo by ultrasound (US) imaging. However, this seems to be a method that is not yet properly standardized nor validated. OBJECTIVES: To identify, synthesize, and collate the critical methodological principles that have been described in the literature for US evaluation of deep fascia sliding mobility in vivo in humans. METHODS: A systematic literature search was conducted on ScienceDirect, PubMed (Medline), Web of Science and B-On databases, according to the PRISMA Extension for Scoping Reviews (PRISMA-ScR) guidelines. The OCEBM LoE was used to evaluate the level of evidence of each study. RESULTS: From a total of 104 full-text articles retrieved and assessed for eligibility, 18 papers were included that evaluate the deep fasciae of the thoracolumbar (n = 4), abdominal (n = 7), femoral (n = 4) and crural (n = 3) regions. These studies addressed issues concerning either diagnosis (n = 11) or treatment benefits (n = 7) and presented levels of evidence ranging from II to IV. Various terms were used to describe the outcome measures representing fascial sliding. Also, different procedures to induce fascial sliding, positioning of the individuals being assessed, and features of US devices were used. The US analysis methods included the comparison of start and end frames and the use of cross-correlation software techniques through automated tracking algorithms. These methods had proven to be reliable to measure sliding between TLF, TrA muscle-fascia junctions, fascia lata, and crural fascia, and the adjacent epimysial fascia. However, the papers presented heterogeneous terminologies, research questions, populations, and methodologies. This two-part paper reviews the evidence obtained for the thoracolumbar and abdominal fasciae (Part 1) and for the femoral and crural fasciae (Part 2). CONCLUSION: The US methods used to evaluate deep fascia sliding mobility in vivo in humans include the comparison of start and end frames and the use of cross-correlation software techniques through automated tracking algorithms. These seem reliable methods to measure sliding of some fasciae, but more studies need to be systematized to confirm their reliability for others. Moreover, specific standardized protocols are needed to assess each anatomical region as well as study if age, sex-related characteristics, body composition, or specific clinical conditions influence US results.

Diagnostic ultrasound assessment of deep fascia sliding mobility in vivo: A scoping review - Part 1: Thoracolumbar and abdominal fasciae.

BACKGROUND: Failure of fascial sliding may occur in cases of excessive or inappropriate use, trauma, or surgery, resulting in local inflammation, pain, sensitization, and potential dysfunction. Therefore, the mechanical properties of fascial tissues, including their mobility, have been evaluated in vivo by ultrasound (US) imaging. However, this seems to be a method that is not yet properly standardized nor validated. OBJECTIVES: To identify, synthesize, and collate the critical methodological principles that have been described in the literature for US evaluation of deep fascia sliding mobility in vivo in humans. METHODS: A systematic literature search was conducted on ScienceDirect, PubMed (Medline), Web of Science and B-On databases, according to the PRISMA Extension for Scoping Reviews (PRISMA-ScR) guidelines. The OCEBM LoE was used to evaluate the level of evidence of each study. RESULTS: From a total of 104 full-text articles retrieved and assessed for eligibility, 18 papers were included that evaluate the deep fasciae of the thoracolumbar (n = 4), abdominal (n = 7), femoral (n = 4) and crural (n = 3) regions. These studies addressed issues concerning either diagnosis (n = 11) or treatment benefits (n = 7) and presented levels of evidence ranging from II to IV. Various terms were used to describe the outcome measures representing fascial sliding. Also, different procedures to induce fascial sliding, positioning of the individuals being assessed, and features of US devices were used. The US analysis methods included the comparison of start and end frames and the use of cross-correlation software techniques through automated tracking algorithms. These methods had proven to be reliable to measure sliding between TLF, TrA muscle-fascia junctions, fascia lata, and crural fascia, and the adjacent epimysial fascia. However, the papers presented heterogeneous terminologies, research questions, populations, and methodologies. This two-part paper reviews the evidence obtained for the thoracolumbar and abdominal fasciae (Part 1) and for the femoral and crural fasciae (Part 2). CONCLUSION: The US methods used to evaluate deep fascia sliding mobility in vivo in humans include the comparison of start and end frames and the use of cross-correlation software techniques through automated tracking algorithms. These seem reliable methods to measure sliding of some fasciae, but more studies need to be systematized to confirm their reliability for others. Moreover, specific standardized protocols are needed to assess each anatomical region as well as study if age, sex-related characteristics, body composition, or specific clinical conditions influence US results.

Foraging and Spatial Ecology of a Polydomous Carpenter Ant, Camponotus leydigi (Hymenoptera: Formicidae), in Tropical Cerrado Savanna: A Natural History Account.

Carpenter ants (genus Camponotus) are considered to be predominantly omnivorous, mixing several feeding habits that include predation, scavenging of animal matter, and plant-derived resources. Nitrogen acquisition is crucial for the nutritional ecology of ant colonies because growing larvae require sustainable protein provisioning. Here, we investigate the foraging ecology and the spatial nesting structure of the carpenter ant, Camponotus leydigi Forel, in Brazilian cerrado savanna. By marking workers from different nests with distinct colors, we revealed that C. leydigi occupies physically separated but socially connected nests (up to 30 m apart), a phenomenon known as polydomy. Observational data on aboveground internest movements in C. leydigi corroborate cooperative exchanges between nest units and confirm several types of social connections, including internest transfer of liquid and solid food, transport of colony members (brood, workers), movement of solitary workers, and internest recruitment. Polydomous C. leydigi allocate foragers throughout 1,700 m2, feeding mostly on termites and plant-derived exudates. Influx of exudates is threefold higher compared with solid food. Uric acid pellets excreted by lizards comprise 20% of the solid diet in C. leydigi, a rare quantitative assessment of this peculiar type of nitrogen complementation in ants. Based on video recordings, we hypothesize that nest decentralization in C. leydigi may reduce foraging constraints caused by overt interference by the aggressive ant, Ectatomma brunneum Smith, F. (Hymenoptera: Formicidae), which regularly blocks nest entrances. Our field study enhances the importance of natural history data to clarify selective pressures underlying the evolution of particular behavioral patterns (nutritional and nesting habits) in ants.

Foraging Ecology of the Leaf-Cutter Ant, Acromyrmex subterraneus (Hymenoptera: Formicidae), in a Neotropical Cerrado Savanna.

Fungus-farming ants cultivate a fungal symbiont inside the nest that serves as a food source. Leaf-cutter ants are distinctive among fungus-farmers because they forage for fresh plant material to nurture the fungus. Here we investigate the foraging ecology of Acromyrmex subterraneus (Forel) in the Brazilian cerrado savanna. We examined the species activity pattern, forage material collected, and the relationship between load mass and forager size. Ant activity peaked at night and was negatively related to temperature but positively related to relative air humidity. The majority of the items collected by ants was plant material: dry and fresh leaves, flowers, and fruits. Trunk trails ranged from 0.7 to 13 m and colony home ranged from 2 to 28 m2, indicating that ants collect material nearby the nest. Total load mass was positively associated with forager size, especially in the case of leaves. The negative relationship between ant size and burden suggests that ants might optimize their delivery rate by collecting lighter substrates more frequently. Given their pest status, most studies on leaf-cutters are undertaken in human-altered environments. Information on A. subterraneus in native cerrado is imperative given the threatened status of this vegetation. Leaf-cutters thrive in disturbed cerrado and severe seedling herbivory may hinder vegetation recovery. Our fieldwork may provide insights for management techniques of Acromyrmex colonies in agroecosystems, as well as for restoration programs of degraded cerrado areas.