Anoxic-biocathode microbial desalination cell as a new approach for wastewater remediation and clean water production.

Bioelectrochemical systems are emerging as a promising and friendly alternative to convert the energy stored in wastewater directly into electricity by microorganisms and utilize it in situ to drive desalination. To better understand such processes, we propose the development of an anoxic biocathode microbial desalination Cell for the conversion of carbon- and nitrogen-rich wastewaters into bioenergy and to perform salt removal. Our results demonstrate a power output of 0.425 W m-3 with desalination, organic matter removal and nitrate conversion efficiencies of 43.69, 99.85 and 92.11% respectively. Microbiological analysis revealed Proteobacteria as the dominant phylum in the anode (88.45%) and biocathode (97.13%). While a relatively higher bacterial abundance was developed in the anode chamber, the biocathode showed a greater variety of microorganisms, with a predominance of Paracoccus (73.2%), which are related to the denitrification process. These findings are promising and provide new opportunities for the development and application of this technology in the field of wastewater treatment to produce cleaner water and conserve natural resources.

Effects of dicyandiamide and Mg/P on the global warming potential of swine slurry and sawdust cocomposting.

Composting is an emerging strategy for swine slurry treatment; nonetheless, significant greenhouse gases (GHG) emissions may occur during this process. We carried out two separate assays with increasing doses of dicyandiamide (DCD; up to 1.1% w/w) as a nitrification inhibitor and solutions of MgCl2 and H3PO4 (Mg/P; up to 0.09/0.06 mol kg-1) to promote struvite crystallization in order to assess their efficiencies as additives to decrease GHG emission during swine slurry cocomposting with sawdust (1:1v/v). We monitored the nitrous oxide (N2O-N), methane (CH4-C), and carbon dioxide (CO2-C) emissions and the ammonia (NH4+-N) and nitrate/nitrite (NOx-N) concentrations in compost reactors (35 L) during the first 4-5 weeks of composting. DCD had no effect on CH4-C and CO2-C emissions but decreased N2O-N losses by up to 56% compared with control. However, DCD inactivation was favored by thermophilic conditions and N2O-N emissions increased to same levels of control after 13 days. Mg/P was effective to decrease N2O-N losses only at the highest dose, which also sustained higher [NH4+-N] in the compost by the end of the assessment. Nonetheless, the use of 0.09/0.06 mol kg-1 of Mg/P also decreased CH4-C and CO2-C emissions compared with lower doses of Mg/P and unamended treatments. Overall, DCD and Mg/P amendments decreased the global warming potential (GWP) of swine slurry composting by up to 46 and 28%, respectively. The Mg/P application may be also interesting to increase the compost quality by increasing its NH4+-N availability. Graphical abstract.

Prospects in bioelectrochemical technologies for wastewater treatment.

Bioelectrochemical technologies are emerging as innovative solutions for waste treatment, offering flexible platforms for both oxidation and reduction reaction processes. A great variety of applications have been developed by utilizing the energy produced in bioelectrochemical systems, such as direct electric power generation, chemical production or water desalination. This manuscript provides a literature review on the prospects in bioelectrochemical technologies for wastewater treatment, including organic, nutrients and metals removal, production of chemical compounds and desalination. The challenges and perspectives for scale-up were discussed. A technological strategy to improve the process monitoring and control based on big data platforms is also presented. To translate the viability of wastewater treatment based on bioelectrochemical technologies into commercial application, it is necessary to exploit interdisciplinary areas by combining the water/wastewater sector, energy and data analytics technologies.

Biological fuel cells produce bioelectricity with in-situ brackish water purification.

Biological fuel cells, namely microbial desalination cells (MDCs) are a promising alternative to traditional desalination technologies, as microorganisms can convert the energy stored in wastewater directly into electricity and utilize it in situ to drive desalination, producing a high-quality reuse water. However, there are several challenges to be overcome in order to scale up from laboratory research. This study was conducted in order to better understand the performance of MDCs inoculated with marine sediments during the treatment of brackish water (5.0 g L-1 of NaCl) under three different configurations and cycles of desalination, envisaging the future treatment of saline wastewaters with conductivities lower than 10 mS cm-1. Results have shown that by increasing the desalination cycle three times, the efficiency of salt removal was improved by 3.4, 2.4 and 2.3 times for 1-MDC, 3-MDC, and 5-MDC, respectively. The same trend was observed for electrochemical data. Findings encourage further development of the MDC for sustainable brackish water and wastewater purification and future on-site utilization.

Bacterial Endospores as Phage Genome Carriers and Protective Shells.

Bacterial endospores can serve as phage genome protection shells against various environmental stresses to enhance microbial control applications. The genomes of polyvalent lytic Bacillus phages PBSC1 and PBSC2, which infect both B. subtilis subsp. subtilis and B. cereus NRS 248, were incorporated into B. subtilis endospores (without integration into the host chromosome). When PBSC1 and PBSC2 were released from germinating endospores, they significantly inhibited the growth of the targeted opportunistic pathogen B. cereus Optimal endospore entrapment was achieved when phages were introduced to the fast-sporulating prespores at a multiplicity of infection of 1. Longer endospore maturation (48 h versus 24 h) increased both spore yield and efficiency of entrapment. Compared with free phages, spore-protected phage genomes showed significantly higher resistance toward high temperatures (60 to 80°C), extreme pH (pH 2 or pH 12), and copper ions (0.1 to 10 mg/liter). Endospore germination is inducible by low concentrations of l-alanine or by a germinant mixture (l-asparagine, d-glucose, d-fructose, and K+) to trigger the expression, assembly, and consequent release of phage particles within 60 to 90 min. Overall, the superior resiliency of polyvalent phages protected by endospores might enable nonrefrigerated phage storage and enhance phage applications after exposure to adverse environmental conditions.IMPORTANCE Bacteriophages are being considered for the control of multidrug-resistant and other problematic bacteria in environmental systems. However, the efficacy of phage-based microbial control is limited by infectivity loss during phage delivery and/or storage. Here, we exploit the pseudolysogenic state of phages, which involves incorporation of their genome into bacterial endospores (without integration into the host chromosome), to enhance survival in unfavorable environments. We isolated polyvalent (broad-host-range) phages that efficiently infect both benign and opportunistically pathogenic Bacillus strains and encapsulated the phage genomes in B. subtilis endospores to significantly improve resistance to various environmental stressors. Encapsulation by spores also significantly enhanced phage genome viability during storage. We also show that endospore germination can be induced on demand with nutrient germinants that trigger the release of active phages. Overall, we demonstrate that encapsulation of polyvalent phage genomes into benign endospores holds great promise for broadening the scope and efficacy of phage biocontrol.

Magnetite nanoparticles influence the ammonium-oxidizing bacteria activity during nitritation process.

With nanotechnology dissemination, nanomaterials' (NMs) release into the environment is inevitable and may adversely affect the wastewater treatment processes. Among the NMs, the iron oxide nanoparticles have a considerable commercial potential, mainly because their magnetic properties, high catalytic ability and antimicrobial activity. However, few studies have examined their potential effect on the biological wastewater treatment. In this process, ammonium-oxidizing bacteria (AOB) are sensitive to the presence of inhibitory compounds and are useful as biosensors to assess contaminant toxicity information. Thus, this work aimed to assess the effect of commercial magnetite nanoparticles (Fe3O4-NPs) on AOB activity. Kinetic experiments were carried out where AOB were exposed in a short-term period (14 h) to different concentrations (from 0.2 to 1.0 g L-1) of Fe3O4-NPs. A decrease of the 61.33% in the NO2--N production rate was observed to the highest concentration of Fe3O4-NPs studied, compared with the control sample. The Fe3O4-NPs concentration that reduces 50% of NO2--N production rate (IC-50) was estimated 0.483 g Fe3O4-NP L-1. Scanning electron microscopy images revealed that NPs remained incorporated in the biomass (sludge). These results suggest that NPs can reach the environment through sludge disposal, mainly in cases of the reuse as soil fertilizer.

Kinetic models for nitrogen inhibition in ANAMMOX and nitrification process on deammonification system at room temperature.

In this study were fitted the best kinetic model for nitrogen removal inhibition by ammonium and/or nitrite in three different nitrogen removal systems operated at 25 °C: a nitrifying system (NF) containing only ammonia oxidizing bacteria (AOB), an ANAMMOX system (AMX) containing only ANAMMOX bacteria, and a deammonification system (DMX) containing both AOB and ANAMMOX bacteria. NF system showed inhibition by ammonium and was best described by Andrews model. The AMX system showed a strong inhibition by nitrite and Edwards model presented a best system representation. For DMX system, the increased substrate concentration (until 1060 mg NH3-N/L) tested was not limiting for the ammonia consumption rate and the Monod model was the best model to describe this process. The AOB and ANAMMOX sludges combined in the DMX system displayed a better activity, substrate affinity and excellent substrate tolerance than in nitrifying and ANAMMOX process.

Assessment of N2O emission from a photobioreactor treating ammonia-rich swine wastewater digestate.

This study investigated the interactions between naturally occurring bacteria and the microalgae Chlorella vulgaris within a lab scale photobioreactor treating ammonia-rich swine wastewater digestate effluent. Nitrification and denitrification were assessed by targeting ammonia monoxygenases (amoA), nitrate (narG), nitrite (nirS), nitric oxide (norB) and nitrous oxide (nosZ) reductases genes. Oxygen produced from microalgae photosynthesis stimulated nitrification. Under limiting carbon availability (i.e., <1.44 for mg TOC/mg NO2-N and 1.72 for mg TOC/mg NO3-N), incomplete denitrification led to accumulation of NO2 and NO3. Significant N2O emission (up to 118 µg N2O-N) was linked to NO2 metabolism in Chlorella. The addition of acetate as external carbon source recovered heterotrophic denitrification activity suppressing N2O emission. Effluent methane concentrations trapped within photobioreactor was removed concomitantly with ammonia. Overall, closed photobioreactors can be built to effectively remove nitrogen and mitigate simultaneously greenhouse gases emissions that would occur otherwise in open microalgae-based wastewater treatment systems.