Proximate and fatty acid profile analysis of Tenebrio molitor and Zophobas morio using different killing methods.

The present work aimed to quantify the macronutrients and the fatty acid (FA) profile in different killing methods, blanching (BC) and freezing (FR), on edible insects of the speciesTenebrio molitor(TM) andZophobas morio(ZM). Concerning macronutrients TM-BC and TM-FR presented 51.2% and 50.6% of protein, 28% and 29.4% of lipids, and 12.4% and 11.4%. Meanwhile, ZM-BC and ZM-FR expressed 42.8% and 43.7% of protein, 39.1% and 40.1% of lipids, and 10.7% and 8.9% of carbohydrates. The FA of TM and ZM shows respectively values of Saturated Fatty Acids (∑SFA) 30% - 45%, Monounsaturated (MUFA) 47% - 32%, Polyunsaturated (∑PUFA) 23% - 22%, Atherogenicity Index (AI) 0.64 - 0.75, Thrombogenicity Index (TI) 0.77 - 1.44 and hypocholesterolemic/hypercholesterolemic index (h/H) of 2.50-1.51. Based on the results obtained, the slaughter methods showed statistically differences in relation to MUFA's in TM, and ZM larvae only in the minority fraction of FA.

Fast and low-cost method for direct and simultaneous determination of nitrogen and carbon in soybean leaves using benchtop and portable near-infrared devices.

BACKGROUND: The current techniques for determining carbon and nitrogen content to provide information about the nutritional status of plants are time-consuming and expensive. For this reason, the objective of this study was to develop an analytical method for the direct and simultaneous determination of nitrogen and carbon elemental content in soybean leaves using near-infrared spectroscopy and compare the performance of conventional (1100-2500 nm spectral range) and portable equipment (1100-1700 nm spectral range). Partial least-squares regression models were developed using 27 soybean leaf samples collected during the 2021 harvest and applied for the simultaneous determination of carbon and nitrogen in 13 samples collected during the 2022 harvest. RESULTS: The root-mean-square error of prediction values for nitrogen and carbon were low (2.42 g kg-1 and 4.37 g kg-1 respectively) for the benchtop method yielded low but higher for the portable method (3.82 g kg-1 and 10.7 g kg-1 respectively). The benchtop method did not show significant differences when compared with the reference method for determining nitrogen and carbon. In contrast, the portable methodology showed potential as a screening method for determining nitrogen levels, particularly in fieldwork. CONCLUSION: The methodologies evaluated in this study were implemented and evaluated under real crop monitoring conditions, using independent sets of calibration and prediction samples. Their utilization enables the acquisition of cost-effective, safe analytical data aligning with the principles of green analytical chemistry. © 2023 Society of Chemical Industry.

Hybrid constructed wetlands integrated with microbial fuel cells and reactive bed filter for wastewater treatment and bioelectricity generation.

The present study aimed to develop a pilot-scale integrated system composed of anaerobic biofilter (AF), a floating treatment wetland (FTW) unit, and a vertical flow constructed wetland coupled with a microbial fuel cell (CW-MFC) and a reactive bed filter (RBF) for simultaneously decentralized urban wastewater treatment and bioelectricity generation. The first treatment stage (AF) had 1450 L and two compartments: a settler and a second one filled with plastic conduits. The two CWs (1000 L each) were vegetated with mixed plant species, the first supported in a buoyant expanded polyethylene foam and the second (CW-MFC) filled with pebbles and gravel, whereas the RBF unit was filled with P adsorbent material (light expanded clay aggregate, or LECA) and sand. In the CW-MFC units, 4 pairs of electrode chambers were placed in different spacing. First, both cathode and anode electrodes were composed of graphite sticks and monitored as open circuit. Later, the cathode electrodes were replaced by granular activated carbon (GAC) and monitored as open and closed circuits. The combined system efficiently reduced COD (> 64.65%), BOD5 (81.95%), N-NH3 (93.17%), TP (86.93%), turbidity (94.3%), and total coliforms (removal of three log units). Concerning bioenergy, highest voltage values were obtained with GAC electrodes, reaching up to 557 mV (open circuit) and considerably lower voltage outputs with closed circuit (23.1 mV). Maximum power densities were obtained with 20 cm (0.325 mW/m2) and 30 cm (0.251 mW/m2). Besides the electrode superficial areas, the HRT and the water level may have influenced the voltage values, impacting DO and COD concentrations in the wastewater.