ABSTRACT
Optical neuritis (ON) is characterized by inflammation of the optic nerve, and is one of the first clinical signs of multiple sclerosis (MS). Experimental autoimmune encephalomyelitis (EAE) is the animal model used to study MS and ON. The present study evaluated the induction, development and progression of ON using an EAE model induced by 100 µg or 300 µg of MOG35-55. An EAE model was induced in C57BL/6 mice by tail base injection of 100 µg or 300 µg of MOG35-55 in complete Freund's adjuvant, supplemented with Mycobacterium tuberculosis. On the day of injection and 48 h later, animals received intraperitoneally 300 ng of pertussis toxin. On days 7, 10, 14, 21 and 58 the optic nerve was dissected for histological analysis, production of CCL5 and immunohistochemical detection of CD4 and CD8. The histological changes observed in the optic nerves consisted of inflammatory cell infiltrates showing varying degrees of ON in the two groups. The onset of ON in the 300 µg of MOG35-55 group was coincident with higher production of CCL5, on day 10 after induction. However, the 100 µg MOG35-55 group showed more intense inflammatory infiltrate on day 14 after induction, with higher amounts of CD4 and CD8, reaching an excessive demyelination process on days 21 and 58 after induction. The results suggest that two different concentrations of MOG35-55 lead to different forms of evolution of optic neuritis.
Subject(s)
Encephalomyelitis, Autoimmune, Experimental/immunology , Inflammation Mediators/administration & dosage , Inflammation Mediators/physiology , Myelin-Oligodendrocyte Glycoprotein/administration & dosage , Optic Neuritis/immunology , Optic Neuritis/pathology , Animals , CD4 Antigens/biosynthesis , CD4-Positive T-Lymphocytes/immunology , CD4-Positive T-Lymphocytes/metabolism , CD4-Positive T-Lymphocytes/pathology , CD8 Antigens/biosynthesis , CD8-Positive T-Lymphocytes/immunology , CD8-Positive T-Lymphocytes/metabolism , CD8-Positive T-Lymphocytes/pathology , Chemokine CCL5/biosynthesis , Disease Models, Animal , Dose-Response Relationship, Drug , Encephalomyelitis, Autoimmune, Experimental/pathology , Female , Humans , Inflammation/immunology , Inflammation/metabolism , Inflammation/pathology , Mice , Mice, Inbred C57BL , Multiple Sclerosis/immunology , Multiple Sclerosis/metabolism , Multiple Sclerosis/pathology , Myelin-Oligodendrocyte Glycoprotein/physiology , Optic Neuritis/metabolism , Peptide Fragments/administration & dosage , Peptide Fragments/physiologyABSTRACT
Objetivo: avaliar a freqüência de manifestaçöes oculares observadas em pacientes soropositivos para HTLV-I no Rio de Janeiro. Métodos: O estudo abrangeu 17 pacientes portadores de TSP/HAM (paraparesia tropical espástica/ mielopatia associada ao HTLV-I) e 55 pacientes soropositivos para HTLV-I não portadores de TSP/HAM ou ATLL (leucemia/linfoma de células T do adulto).Resultados: Nos pacientes portadores de TSP/HAM foi observada a freqüência de 11,8 porcento de uveíte anterior, 11,8 porcento de vasculite retiniana e 5,9 porcento de opacidade vítrea. No grupo de pacientes soropositivos para HTLV-I näo portadores de TSP/HAM ou ATLL, observou-se a freqüência de 1,8 porcento de vasculite retiniana e 1,8 porcento de exsudato algodonoso. Conclusäo: Concluiu-se que, em tais manifestaçöes oculares, o HTLV-I deve ser considerado como um dos agentes etiológicos a serem pesquisados em áreas endêmicas como o Rio de Janeiro
