ABSTRACT
Trichoderma viride C-1 strain was irradiated with UV until the survival level of 0.03% was obtained. Sixteen mutants were isolated on the basis of the visible clearance zone around the colonies on the media with cellulose and glycerol. Then they were cultivated on a rotary shaker in the liquid Saunders medium supplemented with microcrystalline cellulose and comminuted sugar beet pulp. Exo-1,4-beta-glucanase, endo-1,4-beta-glucanase and beta-glucosidase were assayed in the supernatants of postcultural liquids at different time intervals of culture. The same mutants were characterized by higher biosynthesis level of exoglucanase (1.2-5.0 times) endoglucanase (1.2-2.5 times) and beta-glucosidase (1.5-1.7 times) when compared with the wild type strain.
Subject(s)
Cellulase/metabolism , Mitosporic Fungi/enzymology , Mutation , Trichoderma/enzymology , Trichoderma/genetics , Trichoderma/radiation effects , Ultraviolet RaysABSTRACT
The dynamics of the utilization of hydrocarbons from a synthetic mixture used as sole carbon and energy source in medium during the growth of two strains of yeast, Candida scottii A-31 and Candida rugosa A-7, was studied. The yeasts grew in a mineral medium with 2% of hydrocarbons, on a shaker, for 72 hours. During this time almost complete utilization of n-paraffins in the medium was observed and the biomass increments were 11.2 and 12.64 g of dry wt./l, respectively. The examined strains did not utilize iso-paraffins but were found to metabolize n-paraffins at various rates.
