Toxicity in semiarid sediments influenced by tailings of an abandoned gold mine.

The mining district of El Triunfo (ET-MD) has an estimated 800,000 t of mine wastes scattered in the environment, contaminating the sediment with potentially toxic elements such as As, Cd, Pb, and Zn. In order to estimate the toxicity of the sediment to the adjacent biota, the aims of our study are to calculate the mortality and inhibition through bioassays, using sediment, and test organisms such as Daphnia magna and Selenastrum capricornutum (Pseudokirchneriella subcapitata), respectively. The D. magna mortality was 31 ± 12% and the S. capricornutum growth inhibition was 53 ± 24%. The contamination of the sediment determines the high mortality of D. magna and the high inhibition of S. capricornutum in the system, indicating risk for the biota in the contaminated system.

Detoxification and mineralization of Acid Blue 74: study of an alternative secondary treatment to improve the enzymatic decolourization.

Many reports describe the decolourization of dyes by fungal enzymes. However, these enzymes do not contribute to dye mineralization but only to its biotransformation into less coloured or colourless molecules persisting in solution. Therefore, it is essential to analyse the identity of the metabolites produced during enzymatic treatments and its biodegradation into an appropriate system. The present work examines the decolourization/detoxification of a simulated effluent (containing Acid Blue 74) by fungal enzymes and proposes a secondary treatment using an anaerobic system to improve the enzymatic decolourization through the complete mineralization of the dye. Ligninolytic enzymes were produced by solid culture using the thermo-tolerant fungus Fomes sp. EUM1. The enzymes produced showed a high rate of decolourization (>95 % in 5 h) and were stable at elevated temperature (40 °C) and ionic strength (NaCl, 50 mM). Isatin-5-sulphonic acid was identified via (1)H-NMR as oxidation product; tests using Daphnia magna revealed the non-toxic nature of this compound. To improve the enzymatic degradation and avoid coupling reactions between the oxidation products, the effluent was subjected to an anaerobic (methanogenic) treatment, which achieved high mineralization efficiencies (>85 %). To confirm the mineralization of isatin-5-sulphonic acid, a specific degradation study, which has not been reported before, with this single compound was conducted under the same conditions; the results showed high removal efficiencies (86 %) with methane production as evidence of mineralization. These results showed the applicability of an anaerobic methanogenic system to improve the enzymatic decolourization/detoxification of Acid Blue 74 and achieve its complete mineralization.