Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 7 de 7
Filter
Add more filters










Database
Language
Publication year range
1.
Tuberculosis (Edinb) ; 130: 102124, 2021 09.
Article in English | MEDLINE | ID: mdl-34488079

ABSTRACT

The purpose of the present study was to evaluate a real-time PCR system for 12 nontuberculous mycobacteria (NTM) species identification developed by Central Tuberculosis Research Institute (CTRI; Moscow, Russia) in cooperation with Syntol LLC (Moscow, Russia). NTM cultures (210 strains, 19 species), Mycobacterium tuberculosis complex (MTBC) cultures (21 strains, 2 species), non-mycobacterial microorganisms (18 strains, 13 species) were used for the first stage of the assay evaluation. Clinical samples (sputum, N = 973) positive for smear microscopy and MTBC/NTM DNA by a PCR-based screening assay collected from 819 patients were used for specificity and sensitivity evaluation. Sensitivity for determining the NTM species directly from diagnostic material was 99.71%, with the specificity of 100%. The sensitivity and specificity for NTM species identification in cultures was 99.67% and 100%, respectively. Both sensitivity and specificity for determining MTBC in cultures was 100%.


Subject(s)
Mycobacterium Infections, Nontuberculous/diagnosis , Nontuberculous Mycobacteria/isolation & purification , Real-Time Polymerase Chain Reaction , Humans , Mycobacterium Infections, Nontuberculous/microbiology , Nontuberculous Mycobacteria/classification , Sensitivity and Specificity , Sputum/microbiology
2.
Tuberculosis (Edinb) ; 114: 17-23, 2019 01.
Article in English | MEDLINE | ID: mdl-30711153

ABSTRACT

The purpose of the present study was to create a real-time PCR test system allowing simultaneous detection of nontuberculous mycobacteria (NTM) and Mycobacterium tuberculosis complex (MTBC) both in culture and sputum. NTM cultures (18 strains, 18 species), MTBC cultures (16 strains, 2 species) and non-mycobacterial microorganisms from the collection of the Central Research TB Institute (CTRI) were used for the preliminary evaluation of the test system. 301 NTM cultures from patients with mycobacteriosis were used to assess the sensitivity of the developed test system. Clinical respiratory samples (sputum) from 104 patients with mycobacteriosis, 3627 patients with tuberculosis and 118 patients with other lung diseases were used for diagnostic sensitivity and specificity testing. The specificity and sensitivity of the assay for MTBC was found to be 100% both in culture and sputum samples; for NTM, the specificity was 100% in culture and sputum, the sensitivity reached 100% in culture and 73.1% in sputum samples. Positive predictive value (PPV) and negative predictive value (NPV) of the assay for culture were both 100%, for clinical material 100% and 80.8%, respectively. The limit of detection at the probability of detection 95% (LoD95%) was estimated to be 16 cfu/ml for M. tuberculosis H37RV and 1200 cfu/ml for M. avium.


Subject(s)
Mycobacterium Infections, Nontuberculous/diagnosis , Mycobacterium tuberculosis/isolation & purification , Nontuberculous Mycobacteria/isolation & purification , Tuberculosis/diagnosis , Bacterial Typing Techniques/methods , DNA, Bacterial/isolation & purification , Diagnosis, Differential , Humans , Mycobacterium tuberculosis/classification , Mycobacterium tuberculosis/genetics , Nontuberculous Mycobacteria/classification , Nontuberculous Mycobacteria/genetics , Predictive Value of Tests , Real-Time Polymerase Chain Reaction/methods , Sensitivity and Specificity , Sputum/microbiology
3.
J Neurophysiol ; 105(1): 117-29, 2011 Jan.
Article in English | MEDLINE | ID: mdl-20980543

ABSTRACT

Brain damage causes multiple changes in synaptic function and intrinsic properties of surviving neurons, leading to the development of chronic epilepsy. In the widely used pilocarpine-status epilepticus (SE) rat model of temporal lobe epilepsy (TLE), a major alteration is the marked increase in the fraction of intrinsically bursting CA1 pyramidal cells. Here we have differentiated between two types of bursting phenotypes: 1) bursting in response to threshold-straddling excitatory current pulses (low-threshold bursting) and 2) bursting only in response to suprathreshold stimuli (high-threshold bursting). Low-threshold bursting prevailed in 46.5% of SE-experienced neurons sampled 1-4 wk after pilocarpine-SE, but was rarely seen in control neurons (1.9%). As previously shown, it appeared to be driven predominantly by a T-type Ca(2+) current (I(CaT)) in the apical dendrites. After blocking low-threshold bursting with Ni(2+), the same neurons still manifested a high-threshold bursting phenotype. Another 40.1% of SE-experienced neurons displayed only a high-threshold bursting phenotype and the remaining 13.4% of these neurons were nonbursters. Altogether, high-threshold bursting prevailed in 86.6% of SE-experienced neurons, but only in 33.0% of control neurons. Several lines of evidence indicated that high-threshold bursting is driven by persistent Na(+) current (I(NaP)) at or near the soma. Congruently, I(NaP) was 1.5-fold larger in SE-experienced versus control neurons. We conclude that an increase in I(NaP), conjointly with an increase in I(CaT), strongly contributes to the predominance of bursting phenotypes in CA1 pyramidal cells early after pilocarpine-SE and thus likely plays a role in the development of a chronic epileptic condition in this TLE model.


Subject(s)
CA1 Region, Hippocampal/physiopathology , Neurons/physiology , Sodium Channels/physiology , Status Epilepticus/physiopathology , Animals , CA1 Region, Hippocampal/cytology , CA1 Region, Hippocampal/drug effects , Calcium Channel Blockers/pharmacology , Calcium Channels, T-Type/drug effects , Calcium Channels, T-Type/physiology , Male , Models, Animal , Neurons/drug effects , Patch-Clamp Techniques , Pilocarpine/adverse effects , Rats , Rats, Inbred Strains , Rats, Wistar , Sodium Channel Blockers/pharmacology , Sodium Channels/drug effects , Status Epilepticus/chemically induced , Tetrodotoxin/pharmacology , Time Factors
4.
J Neurosci ; 28(49): 13341-53, 2008 Dec 03.
Article in English | MEDLINE | ID: mdl-19052226

ABSTRACT

In both humans and animals, an insult to the brain can lead, after a variable latent period, to the appearance of spontaneous epileptic seizures that persist for life. The underlying processes, collectively referred to as epileptogenesis, include multiple structural and functional neuronal alterations. We have identified the T-type Ca(2+) channel Ca(v)3.2 as a central player in epileptogenesis. We show that a transient and selective upregulation of Ca(v)3.2 subunits on the mRNA and protein levels after status epilepticus causes an increase in cellular T-type Ca(2+) currents and a transitional increase in intrinsic burst firing. These functional changes are absent in mice lacking Ca(v)3.2 subunits. Intriguingly, the development of neuropathological hallmarks of chronic epilepsy, such as subfield-specific neuron loss in the hippocampal formation and mossy fiber sprouting, was virtually completely absent in Ca(v)3.2(-/-) mice. In addition, the appearance of spontaneous seizures was dramatically reduced in these mice. Together, these data establish transcriptional induction of Ca(v)3.2 as a critical step in epileptogenesis and neuronal vulnerability.


Subject(s)
Calcium Channels, T-Type/genetics , Calcium Signaling/genetics , Epilepsy, Temporal Lobe/genetics , Hippocampus/metabolism , Neurons/metabolism , Up-Regulation/genetics , Animals , Calcium Channels, T-Type/metabolism , Channelopathies/genetics , Channelopathies/metabolism , Channelopathies/physiopathology , Disease Models, Animal , Epilepsy, Temporal Lobe/chemically induced , Epilepsy, Temporal Lobe/physiopathology , Gene Expression Regulation/drug effects , Gene Expression Regulation/genetics , Genetic Predisposition to Disease/genetics , Hippocampus/physiopathology , Male , Mice , Mice, Knockout , Mossy Fibers, Hippocampal/metabolism , Mossy Fibers, Hippocampal/physiopathology , Muscarinic Agonists/pharmacology , Nerve Degeneration/genetics , Nerve Degeneration/metabolism , Nerve Degeneration/physiopathology , Neurons/drug effects , Pilocarpine/pharmacology , Protein Subunits/genetics , Protein Subunits/metabolism , Rats , Rats, Wistar , Transcriptional Activation/genetics
5.
Eur J Neurosci ; 18(10): 2792-802, 2003 Nov.
Article in English | MEDLINE | ID: mdl-14656328

ABSTRACT

Epileptic activity evokes profound alterations of hippocampal organization and function. Genomic responses may reflect immediate consequences of excitatory stimulation as well as sustained molecular processes related to neuronal plasticity and structural remodeling. Using oligonucleotide microarrays with 8799 sequences, we determined subregional gene expression profiles in rats subjected to pilocarpine-induced epilepsy (U34A arrays, Affymetrix, Santa Clara, CA, USA; P < 0.05, twofold change, n = 3 per stage). Patterns of gene expression corresponded to distinct stages of epilepsy development. The highest number of differentially expressed genes (dentate gyrus, approx. 400 genes and CA1, approx. 700 genes) was observed 3 days after status epilepticus. The majority of up-regulated genes was associated with mechanisms of cellular stress and injury - 14 days after status epilepticus, numerous transcription factors and genes linked to cytoskeletal and synaptic reorganization were differentially expressed and, in the stage of chronic spontaneous seizures, distinct changes were observed in the transcription of genes involved in various neurotransmission pathways and between animals with low vs. high seizure frequency. A number of genes (n = 18) differentially expressed during the chronic epileptic stage showed corresponding expression patterns in hippocampal subfields of patients with pharmacoresistant temporal lobe epilepsy (n = 5 temporal lobe epilepsy patients; U133A microarrays, Affymetrix; covering 22284 human sequences). These data provide novel insights into the molecular mechanisms of epileptogenesis and seizure-associated cellular and structural remodeling of the hippocampus.


Subject(s)
Epilepsy, Temporal Lobe/metabolism , Gene Expression , Hippocampus/metabolism , Pilocarpine/analogs & derivatives , Animals , Cluster Analysis , Disease Models, Animal , Epilepsy, Temporal Lobe/chemically induced , Epilepsy, Temporal Lobe/genetics , Hippocampus/anatomy & histology , Humans , Male , Oligonucleotide Array Sequence Analysis/methods , RNA, Messenger/metabolism , Rats , Rats, Wistar , Reverse Transcriptase Polymerase Chain Reaction , Time Factors
6.
J Physiol ; 542(Pt 3): 699-710, 2002 Aug 01.
Article in English | MEDLINE | ID: mdl-12154172

ABSTRACT

Different subtypes of voltage-dependent Ca(2+) currents in native neurones are essential in coupling action potential firing to Ca(2+) influx. For most of these currents, the underlying Ca(2+) channel subunits have been identified on the basis of pharmacological and biophysical similarities. In contrast, the molecular basis of R-type Ca(2+) currents remains controversial. We have therefore examined the contribution of the Ca(V)2.3 (alpha(1E)) subunits to R-type currents in different types of central neurones using wild-type mice and mice in which the Ca(V)2.3 subunit gene was deleted. In hippocampal CA1 pyramidal cells and dentate granule neurones, as well as neocortical neurones of wild-type mice, Ca(2+) current components resistant to the combined application of omega-conotoxin GVIA and MVIIC, omega-agatoxin IVa and nifedipine (I(Ca,R)) were detected that were composed of a large R-type and a smaller T-type component. In Ca(V)2.3-deficient mice, I(Ca,R) was considerably reduced in CA1 neurones (79 %) and cortical neurones (87 %), with less reduction occurring in dentate granule neurones (47 %). Analysis of tail currents revealed that the reduction of I(Ca,R) is due to a selective reduction of the rapidly deactivating R-type current component in CA1 and cortical neurones. In all cell types, I(Ca,R) was highly sensitive to Ni(2+) (100 microM: 71-86 % block). A selective antagonist of cloned Ca(V)2.3 channels, the spider toxin SNX-482, partially inhibited I(Ca,R) at concentrations up to 300 nM in dentate granule cells and cortical neurones (50 and 57 % block, EC(50) 30 and 47 nM, respectively). I(Ca,R) in CA1 neurones was significantly less sensitive to SNX-482 (27 % block, 300 nM SNX-482). Taken together, our results show clearly that Ca(V)2.3 subunits underlie a significant fraction of I(Ca,R) in different types of central neurones. They also indicate that Ca(V)2.3 subunits may give rise to Ca(2+) currents with differing pharmacological properties in native neurones.


Subject(s)
Calcium Channels, R-Type/physiology , Calcium Channels, T-Type/physiology , Hippocampus/metabolism , Neocortex/metabolism , Neurons/metabolism , Animals , Calcium Channel Blockers/pharmacology , Calcium Channels, R-Type/deficiency , Calcium Channels, R-Type/genetics , Calcium Channels, T-Type/drug effects , Dentate Gyrus/metabolism , Drug Resistance , Electric Conductivity , Electrophysiology , Membrane Transport Proteins , Mice , Mice, Knockout/genetics , Pyramidal Cells/metabolism , Reference Values
7.
J Neurosci ; 22(9): 3645-55, 2002 May 01.
Article in English | MEDLINE | ID: mdl-11978840

ABSTRACT

A single episode of status epilepticus (SE) causes numerous structural and functional changes in the brain that can lead to the development of a chronic epileptic condition. Most studies of this plasticity have focused on changes in excitatory and inhibitory synaptic properties. However, the intrinsic firing properties that shape the output of the neuron to a given synaptic input may also be persistently affected by SE. Thus, 54% of CA1 pyramidal cells, which normally fire in a regular mode, are persistently converted to a bursting mode after an episode of SE induced by the convulsant pilocarpine. In this model, intrinsic bursts evoked by threshold-straddling depolarizations, and their underlying spike afterdepolarizations (ADPs), were resistant to antagonists of N-, P/Q-, or L-type Ca2+ channels but were readily suppressed by low (30-100 microm) concentrations of Ni2+ known to block T- and R-type Ca2+ channels. The density of T-type Ca2+ currents, but not of other pharmacologically isolated Ca2+ current types, was upregulated in CA1 pyramidal neurons after SE. The augmented T-type currents were sensitive to Ni2+ in the same concentration range that blocked the novel intrinsic bursting in these neurons (IC50 = 27 microm). These data suggest that SE may persistently convert regular firing cells to intrinsic bursters by selectively increasing the density of a Ni2+-sensitive T-type Ca2+ current. This nonsynaptic plasticity considerably amplifies the output of CA1 pyramidal neurons to synaptic inputs and most probably contributes to the development and expression of an epileptic condition after SE.


Subject(s)
Calcium Channels, T-Type/metabolism , Hippocampus/physiopathology , Neurons/metabolism , Status Epilepticus/physiopathology , Up-Regulation , Action Potentials/drug effects , Action Potentials/physiology , Animals , Calcium Channel Blockers/pharmacology , Calcium Channels, T-Type/drug effects , Cell Separation , Disease Models, Animal , Dose-Response Relationship, Drug , Electric Stimulation , Hippocampus/drug effects , In Vitro Techniques , Male , Membrane Potentials/drug effects , Membrane Potentials/physiology , Neuronal Plasticity/physiology , Neurons/drug effects , Nickel/pharmacology , Patch-Clamp Techniques , Pilocarpine , Rats , Rats, Inbred Strains , Rats, Wistar , Status Epilepticus/chemically induced , Synaptic Transmission/drug effects , Up-Regulation/drug effects
SELECTION OF CITATIONS
SEARCH DETAIL
...