ABSTRACT
This paper deals with the search for optimal conditions for the identification of Transkarbam 12 (T 12) as a substance used for acceleration of transdermal penetration. Such substances are used in cases when drugs do not pass through the skin barrier under normal conditions. Other advantages are that they do not irritate the digestive system, provide continuous administration to an organism, and reduce fluctuations of drug concentration in blood. TLC and HPLC were used for identification. In the case of TLC, Silufol UV254 was used as stationary phase and the mobile phase consisted of chloroform, ethanol, and acetic acid. Detection was performed with iodine vapour. In the case of HPLC, the following three chromatographic columns were tested for the analysis of T 12: Silasorb SPH C 18, Silasorb SPH nitrile, and LiChrosorb Si-60. Because of the absence of any chromophore in the structure of T 12, work was performed on the derivatized compound. Detection was carried out at 230 nm. Quantification was studied on LiChrosorb Si-60 and the linearity, precision, and accuracy were evaluated.
Subject(s)
Carbamates/analysis , Chromatography, High Pressure Liquid/methods , Chromatography, Thin Layer/methods , Administration, Cutaneous , Drug Delivery Systems , HumansABSTRACT
Zirconia-based stationary phases represent an interesting alternative to silica-based materials. Two zirconia-based stationary phases were studied as an option for use in drug analysis. The different properties of zirconia material, distinct from RP silica-columns, were employed for the development of a novel and rapid stability monitoring HPLC method. This method enables simultaneous control of possible degradation processes of active substance (ibuprofen) as well as antimicrobial excipients (methyl-and propylparaben). The separation of ibuprofen, its two main degradation products 2-(4-isobutyrylphenyl)propionic acid and 4-isobutylacetophenone, parabens, and 4-hydroxybenzoic acid as their degradation product was successfully accomplished on a Zr-CarbonC18 column using a mobile phase consisting of acetonitrile-phosphate buffer (pH 4.8)-propan-2-ol (27:56:17, v/v/v). Detection was performed at 258 nm and the analysis was completed within 17 minutes.
Subject(s)
Chromatography, High Pressure Liquid/methods , Pharmaceutical Preparations/analysis , Zirconium , Biotransformation , Drug Stability , Ibuprofen/chemistry , Ibuprofen/isolation & purification , Ibuprofen/pharmacokinetics , Parabens/chemistry , Parabens/isolation & purification , Parabens/pharmacokinetics , Pharmaceutical Preparations/chemistry , TemperatureABSTRACT
Supercritical fluid extraction (SFE) was employed to analyze selected anti-inflammatory drugs in plasma. Evaluation of selected drugs (ibuprofen, indomethacin, and flufenamic acid) was performed using the HPLC method on columns with the reverse phase C-18 and detection in the UV region of the spectrum. A study of the conditions of SFE carried out for 30 min at 50 degrees C investigated the magnitude of the pressure of carbon dioxide suitable for drug extraction, the selection of the collecting solvent, and the modification of CO2 with an organic solvent. The results of the study made it possible to determine the optimal procedure for SFE of ibuprofen, indomethacin, and flufenamic acid from plasma, which renders their HPLC quantification possible.
