ABSTRACT
This study is focused on comparing the effect of various energy densities and wavelengths of diode lasers on the proliferation of human gingival fibroblast (HGF) cells in vitro. In this study, 204 sample cells were examined in 4 test groups (laser radiation) and 1 control group (non-laser radiation). The proliferation rate of radiated cells with wavelengths of 635, 660, 808 and 980 nm and the densities of 1, 1.5, 2.5 and 4 J cm-2 was measured after 1, 3 and 5 days using the MTT assay. The proliferation rate of human gingival fibroblast (HGF) cells in test groups was increased on day 1 at wavelengths of 635, 808 and 980 nm and on day 3 at the wavelength of 980 nm compared with the control group. Our findings denoted that the photobiomodulation therapy increased the proliferation rate of HGF. The most desirable laser radiation setting, which led to the highest proliferation rate of the cells, included 980 nm wavelength with 1, 1.5 and 4 J cm-2 energy densities and 635 nm wavelength with 4 J cm-2 energy density.
Subject(s)
Gingiva , Low-Level Light Therapy , Cell Proliferation , Fibroblasts , Humans , Lasers, SemiconductorABSTRACT
Objectives: Toothpastes and mouthwashes contain chemicals that may be harmful to oral tissues. This study assessed the cytotoxicity and antibacterial activity of toothpastes and compare the Iranian and foreign toothpastes and mouthwashes available in the Iranian market in this respect. Materials and Methods: Twenty samples (13 toothpastes and 4 mouthwashes) were selected. The cytotoxicity of 1, 10, and 50 mg/mL of toothpastes and 0.05, 2 and 10 µL of mouthwashes was measured after 1, 15 and 30 min of exposure to human gingival fibroblasts, each in triplicate. The methyl thiazolyl tetrazolium (MTT) assay was used for cytotoxicity testing. The serial dilution method was utilized to determine the minimum inhibitory concentration (MIC) of each sample against Lactobacillus acidophilus (L. acidophilus) and Streptococcus mutans (S. mutans). Two-way ANOVA and Tukey's test were used for data analysis. Results: A significant difference in cytotoxicity was noted among different products (P=0.00). The difference in cytotoxicity of each sample was not significant at 1, 15 and 30 min (P=0.08). The obtained MIC for all toothpastes and mouthwashes was between 0.0039 mg/mL and 0.0156 mg/mL, except for Sensodyne toothpaste and Oral B mouthwash. Conclusion: Some brands of toothpastes have higher cytotoxicity due to their composition, and their cytotoxicity should not be overlooked. The antibacterial activity of the samples was almost equal when they were in contact with L. acidophilus and S. mutans except for the Irsha mouthwash, Sehat, Darugar and Bath toothpastes. The antibacterial effect of toothpastes and mouthwashes increased with an increase in exposure time.
ABSTRACT
AIM: In the present study, we sought to assess the correlation of IKKi gene polymorphisms (rs1539243 and rs12728136) with chronic periodontitis and peri-implantitis in an Iranian population for the first time in dentistry. MATERIALS AND METHODS: This cross-sectional study was conducted on 75 patients with chronic periodontitis, 38 patients with peri-implantitis, and 84 periodontally healthy subjects. Blood samples were taken from all subjects (5 cc venous) and transferred into Falcon tubes containing EDTA. Extracted DNA samples were evaluated with a spectrophotometer (75 ng), transferred into a 96-well plate, and sent to KBioscience Institute in the United Kingdom for analysis. Assessment of IKKi gene polymorphisms was performed at the KBioscience Institute. Data were analyzed using SPSS v19 software. Differences in genotype frequencies and allele among patients and controls were evaluated using the chi-squared test (P<0.05). RESULTS: A significant difference was detected between the peri-implantitis and chronic periodontitis groups in distribution of CC, TC, and TT genotypes in rs1539243 (P<0.05). Statistically significant differences were detected among the three groups in the frequency of T and C alleles for rs1539243 (P=0.03). CONCLUSIONS: Within the limitations of the present study, the results showed a significant difference in IKKi gene polymorphism rs1539243 between the peri-implantitis and chronic periodontitis groups. However, such difference was not observed in rs12728136 between the two groups.
