ABSTRACT
We collected monthly reports on gastrointestinal illness (GII) episodes among 2348 adults in a 1-year cohort in South West Sweden. The GII episodes were collected by SMS (Short Message System) and validated by telephone interviews among the cohort participants and nationwide. The annual incidence was 0.64 and 0.43 cases per person-year for 28-day self-defined GII (any symptom) and acute GII (vomiting and/or ≥3 episodes of diarrhoea), respectively. The incidence was about 20% higher for the 14-day recall, compared with 28-day recall. The duration of illness was on average 2.3 days. We observed a unimodal seasonal distribution of GII, with the highest prevalence during winter. Responses collected by SMS highly correlated with responses collected by telephone. SMS survey was an efficient tool for the collection of repeated estimates of GII.
Subject(s)
Gastrointestinal Diseases/diagnosis , Gastrointestinal Diseases/epidemiology , Self Report , Text Messaging , Adolescent , Adult , Aged , Aged, 80 and over , Cost of Illness , Female , Humans , Incidence , Male , Middle Aged , Pilot Projects , Prospective Studies , Reproducibility of Results , Seasons , Sweden/epidemiology , Young AdultABSTRACT
Glutaredoxins are small heat-stable proteins that are active as glutathione-dependent oxidoreductases and are encoded by two genes, designated GRX1 and GRX2, in the yeast Saccharomyces cerevisiae. We report here that the expression of both genes is induced in response to various stress conditions including oxidative, osmotic, and heat stress and in response to stationary phase growth and growth on non-fermentable carbon sources. Furthermore, both genes are activated by the high-osmolarity glycerol pathway and negatively regulated by the Ras-protein kinase A pathway via stress-responsive STRE elements. GRX1 contains a single STRE element and is induced to significantly higher levels compared to GRX2 following heat and osmotic shock. GRX2 contains two STRE elements, and is rapidly induced in response to reactive oxygen species and upon entry into stationary phase growth. Thus, these data support the idea that the two glutaredoxin isoforms in yeast play distinct roles during normal cellular growth and in response to stress conditions.
