Reference values for variables of fetal cardiocirculatory dynamics at 11-14 weeks of gestation.

OBJECTIVE: Despite the increasing popularity of first-trimester fetal echocardiography, the evaluation of fetal heart function during this period remains challenging. The parameters of normal cardiac function at 11-14 weeks' gestation are not well defined and appropriate reference values have not yet been established. The purpose of this study was to evaluate the fetal cardiocirculatory dynamics during routine first-trimester screening and establish cross-sectional reference ranges for 11-14 weeks' gestation. METHODS: Fetal echocardiography was performed on 202 women with singleton pregnancies at 11 + 0 to 13 + 6 weeks' gestation. Global cardiac function was evaluated using the heart : chest area ratio and Tei index of the left (LV) and right (RV) ventricles. The proportion of isovolumic contraction (ICT%) and ejection (ET%) times of the cardiac cycle, and the outflow velocities described the systolic function. Diastolic function was evaluated by the proportion of relaxation (IRT%) and filling (FT%) times, the ratio of the blood velocity through the atrioventricular valves during early filling (E) and atrial contraction (A) phases of the cardiac cycle, and ductus venosus pulsatility index for veins (DV-PIV). All participants had additional fetal echocardiography in the second trimester and neonatal clinical examination after birth to confirm normality. RESULTS: The mean heart : chest area ratio (0.203 +/- 0.04) and the Tei indices of both ventricles did not vary significantly during weeks 11-14, but the mean Tei index of the LV (0.375 +/- 0.092) was significantly higher than that of the RV (0.332 +/- 0.079) (P = 0.001). The fetal heart rate (FHR) decreased with increasing crown-rump length (CRL) (P < 0.00001). The LV-ICT% did not vary significantly (P = 0.27), LV-IRT% (P = 0.03) and LV-ET% decreased (P = 0.01), whereas the LV-FT% increased (P = 0.02) with CRL. The RV-ET% (P = 0.84) and RV-FT% (P = 0.60) remained relatively stable. The LV-ET% was lower than the RV-ET% (P = 0.0001). The LV (P = 0.004) and RV (P < 0.00001) outflow velocities and E : A ratios of both ventricles (P < 0.0001) increased with advancing gestation. The E-velocity of the LV (P = 0.003) and RV (P = 0.002) increased significantly but the increase in A-velocity was not significant. The outflow velocity (P = 0.008) and E-velocity (P = 0.005) of the RV were higher than that of the LV but the A-velocities were similar (P = 0.066). The mean DV-PIV was 0.97 +/- 0.23 and did not change significantly (P = 0.95) during weeks 11-14. The FHR and DV-PIV did not correlate with the Tei index of either ventricle. CONCLUSION: We have established reference ranges for the noninvasive evaluation of fetal cardiocirculatory dynamics at 11-14 weeks' gestation.

Components of metabolic syndrome and their impact on fetal growth in women with gestational diabetes mellitus.

UNLABELLED: Gestational diabetes mellitus (GDM) is associated with increased maternal insulin resistance. Maternal hyperglycemia is a well known risk factor for fetal overgrowth. However, despite improved glycemia control, macrosomia complicates a significant proportion of diabetic pregnancies, resulting in increased perinatal risk. The aim of our retrospective study was to investigate the association between fetal growth and different maternal metabolic characteristics in women with GDM. The study group included 357 women (singleton pregnancy, and GDM diagnosed following WHO criteria). The following parameters were studied: maternal pre-pregnancy BMI, 75 g OGTT results, HbA(1c), triglycerides (TAG), total, HDL- and LDL-cholesterol levels at admission. Neonatal birth weight and the prevalence of being large for gestational age birth weight (LGA) was an end-point. We found a significant association between birth weight and HbA(1c), TAG, fasting OGTT glycemia, BMI and a birth weight of a large child born previously. BMI and birth weight of a large child was the strongest independent predictors for LGA. A significant increase in birth weight and the prevalence of LGA (from 10.5% to 83.3%) was related to a number of altered maternal metabolic features. CONCLUSIONS: Fetal growth in a diabetic pregnancy is a complex process and maternal metabolic parameters other than glucose levels should be addressed to reduce the risk of macrosomia in these groups of patients.

Leptin gene, leptin gene polymorphisms and body weight in pregnant women with diabetes mellitus type I.

UNLABELLED: There have been several genetic causes of obesity discussed by past authors, among others leptin, that have provided information regarding signaling pathways in energy expenditure in humans. Genetic variants of the leptin gene and its receptor may influence body weight. AIM: To investigate the role of the leptin gene's polymorphism promotion region (2548 G/A) and the leptin gene receptor polymorphism (668 A/G) and its associations with body weight in pregnant women with type 1 diabetes (PGDM-1). METHODS: 78 PGDM-1 were qualified to the study group (SG) which was divided into normal and over-weight individuals according to BMI criteria. The control group (CG) consisted of first trimester healthy pregnant women with normal body weight. Genetic variants of the leptin gene and its receptor were analyzed using PCR-RFLP assays. Within the SG, the following metabolic parameters were estimated: MBG, HbA1C, insulin dose, LDL, HDL, T-CHOL, creatinine, creatinine clearance and blood pressure. RESULTS: There was a trend found among the majority of homozygous A and G variants in LEP -2548 G/A and LEPR 668 A/G in over-weight and obese individuals in comparison to normal-weight subjects (CG). There were no specific differences found in selected first trimester metabolic parameters in relation to patients' genotypes.

Acylation stimulating protein is associated with pregnancy weight gain.

Among the proteins secreted by adipocytes, acylation stimulating protein (ASP), which plays a crucial role in energetic balance regulation, merits particular attention. ASP is a protein of the C3 complement system, responsible for glucose and lipids metabolism in an insulin-independent mechanism. ASP's role during pregnancy and its interactions with pregnancy hormones remains unknown. The lipogenic character of ASP may impose a question as to what extent this hormone participates in pregnant women lipogenesis, and what is the basal and postprandial ASP secretion during the second trimester of pregnancy. The results of the examinations of 26 pregnant women during the second trimester of their first pregnancy were analyzed. Due to the limited data available in the literature, a control group was examined. The group consisted of 8 healthy non-pregnant patients within similar age ranges. Blood samples were collected in order to determine ASP, total cholesterol, HDL, LDL and triglyceride levels. Basal ASP levels present in obese pregnant women (group OBP; 30.20 +/- 2.13 ng/mL) were significantly higher than those in the healthy control group (group LnP; 20.49 +/- 1.97 ng/mL), P<0.05. Mann-Whitney U test- analysis of these group differences indicated that OBP patients had significantly higher ASP levels than controls at 30 (P<0.01), 60 (P<0.01), and 120 (P<0.01) min after a meal. After a meal, the incremental ASP area under the curve in group OBW patients was significantly higher from that observed in control group LnP (718,9 +/- 263,9 ng/mL x 2h vs. 35,1 +/- 14,6 ng/mL x 2h, P<0.05). Basal concentration of triglycerides, total cholesterol and LDL cholesterol were significantly higher in all pregnant women compared to the group of non-obese non-pregnant women. It was found that lipid parameters were highly dependent upon body mass gain during pregnancy. Group OBP demonstrated significantly higher basal concentrations of all parameters of lipid metabolism in comparison with the remaining groups of pregnant patients. In conclusion, we found abnormalities of ASP and lipid profiles in lean, overweight, and obese pregnant women strictly connected with obesity. Acylation stimulating protein correlated with lipid parameters, suggesting increased risk of dyslipidemia in obese pregnant women.

Microvessel density assessment in benign and malignant endometrial changes.

Tumor angiogenesis is believed to be a prognostic indicator associated with tumor growth and metastasis. Microvessel density (MVD) assessment with common endothelial markers such as CD34 has been found to influence prognosis among endometrial carcinoma patients. The CD105/endoglin antibody has been reported to preferentially bind to proliferated endothelial cells in tissues participating in angiogenesis. The aim of this study was to evaluate the quantification of angiogenesis by assessing MVD in endometrial lesions when comparing the performance of anti-CD34 and anti-CD105 in women with benign and malignant endometrial changes. The study included 58 women (37 postmenopausal) with normal, hyperplastic and malignant endometrium in which preoperative transvaginal sonography was performed. Histological results of the removed endometrium were correlated with MVD assessed in "hot areas" where high densities of microvessels were detected within tumoral tissue. Endometrial cancer was confirmed in 37 women (3 premenopausal). Benign hyperplasia (14 cases), secretory or proliferative endometrium (5 cases) or endometrial atrophy (2 cases) was found in the remaining women. Malignant changes were mostly noted as FIGO stage I and II (28 cases) and had a low (1 or 2) histological grade (29 cases). Median MVD's assessed with CD105 and CD34 were 10.4 and 32.3, respectively. Median MVD assessed with CD34 was almost twice higher in women with endometrial cancer than in women with benign endometrium (CD34 MVD = 41.8 vs. 27.6, p=0.004). In cases of CD105 MVD significant differences between women with benign and malignant endometrial changes were also found (CD105 MVD = 11.8, vs. 6.4; p=0.00007). The menopausal status, but not the clinical stage or histological grading was significantly correlated with both CD34 MVD (p=0.02) and CD105 MVD (p=0.0003). A significant correlation was also found between CD34 and CD105 measured MVD (p=0.000001). In conclusion, transition from endometrial hyperplasia to endometrial cancer appears to be accompanied by microvessel density changes. MVD assessed with both CD34 and CD105 antibodies could be used as a potential prognostic factor in women with endometrial cancer. Our study showed that endoglin, by staining the proliferating microvessels could be more specific and sensitive marker for tumor neoangiogenesis than the more commonly used marker, CD34.

Microvessel density and CpG island methylation of the THBS2 gene in malignant ovarian tumors.

We aimed to investigate the role of thrombospondin-2 (THBS2) related angiogenic activity in malignant ovarian tumors and to determine if aberrant methylation associated inactivation is involved in down-regulating THBS2 expression in ovarian cancer. The methylation status of the THBS2 promoter region and microvessel density (MVD) was studied in 70 malignant ovarian tumors and in 15 control ovarian samples. A methylation specific PCR (MSP) method was used to distinguish methylated from unmethylated DNA in the promoter regions of the THBS2 gene. MVD was assessed with anti-CD34 antibodies and the results were compared between tumors with average (AVD) and high (HVD) microvessel density. Alterations in the expression of trombospondin-2 were more often seen in early (FIGO stage I and II ) than in late stage tumors (66% vs. 30%, p=0.01). Age, menopausal status, the histological type and tumor grade did not correlate with thrombospondin-2 expression, however, silencing of THBS2 gene was more often seen in higher rather than in lower grade (50% vs. 28%) cancers and in nonserous rather than in serous (43% vs. 32%) tumors. In 81% of THBS2 mRNA-negative tumors, ahypermethylated promoter region of THBS2 was found (p=0.00003). An unmethylated product of the MSP reaction was more often detected in high grade tumors (93% vs. 76%, p=0.04). The incidence of THBS2 hypermethylation was not related to the tumor histological type, but unmethylated THBS2 was more often found in serous rather than in nonserous tumor (96% vs. 74%, p=0.01). The median MVD in malignant the tumor samples was 21,7 (range: 7.6-55.2). In the group with HVD, 54% were THBS2 mRNAnegative, conversely, in the group with AVD tumors only 26% of the cases had undetectable THSB2 mRNA. A significant correlation between microvessel density and the expression of trombospondin-2 (p=0.009) was found. In the samples with HVD, 51% had hypermethylated THBS2, however methylation pattern had no significant influence on microvessel density. In conclusion, hypermethylation might be responsible for altered expression of thrombospondin-2 in ovarian cancer. The THSB2 methylation pattern had no significant influence on microvessel density.

Epidemiology of peri/intraventricular haemorrhage in newborns at term.

Peri/intraventricular hemorrhage (PIVH) has significant consequences, particularly leading to cases of adverse neurodevelopment. The aim of this study was to asses the epidemiology of peri/intraventricular haemorrhage in newborns born at term. Study was designed and performed according to epidemiological model of case-controlled studies. The study population was based on 2675 full-term neonates (the mean gestational age was 39.1 +/- 1.2 weeks) admitted to Department of Neonatology of Medical University of Silesia in Katowice (Poland) between the years 2003-2005. Periventricular/intraventricular hemorrhage was diagnosed in 392 neonates (14.65%). In this group of neonates 281 (71.68%) were diagnosed as IVH I degrees , 108 (27.55%) as PIVH II degrees , 2 (0.5%) as PIVH III degrees and in 1 neonate (0.25%) as IVH IV degrees . Further research was carried out on 392 neonates who were diagnosed with PIVH (together I degrees -IV degrees ) and 2283 healthy neonate. The study's inclusion criterion was term delivery (after 37(th) week of gestation). Mean gestational age of the group was 39.11 +/-1.26 weeks and ranged from 37 to 43 weeks. The mean gestational age of neonates with PIVH was 38.91 +/- 1.26 week and -38.14 +/- 1.23 week for those neonates without this pathology. Also, the various potential risk factors of PIVH were analyzed such as: gender, newborn's condition in the 5 minutes after birth (assessed by Apgar score), type of delivery (vaginal, cesarean section, vacuum extractor), umbilical collision, intrauterine infection, hypertension, mother's inflammation of urinary tract and infection of upper airways. We postulate that the etiology of PIVH in term neonates is multifactoral. The findings suggest that male gender, lower birth weight and the mode of delivery are associated with the development of PIVH in term neonates.

Doppler study of the peripheral flows in early gestation.

The aim of this study was to determine the first trimester human peripheral arterial and venous blood flow between 5 - 10 weeks of gestation. Two hundred twenty four women with singleton, uncomplicated pregnancies were prospectively studied with transvaginal ultrasound. Ductus venosus, umbilical artery waveforms and pulsatility indexes (PI) were assessed as well as the waveform of the umbilical vein and the mean velocity (V(mean)) of the umbilical artery flow. The heart rate was also obtained and analyzed. The fetal heart rate showed a positive correlation with increasing gestational age R=0.76 (p<0.000001). Recordings from the umbilical artery, umbilical vein and ductus venosus were obtained starting from 7 weeks of gestation. The signal from the ductus venosus presented always as antegrade flow during atrial contractions. The pulsatility index (PI) of DV as well as PI of the umbilical artery remained unchanged during the study (statistically non-significant). The umbilical artery, using Doppler tracing was investigated and an absent diastolic flow was documented in every case. Umbilical artery V(mean) increased from 3.8 + 0.32 cm/s to 9.0 + 0.21 cm/s from 7 to 10 weeks of gestation (p< 0.005). Recordings from the umbilical vein showed the pulsation during atrial contractions. Ductus venosus blood velocity and waveform patterns did not change significantly during the study period. Pulsation in the umbilical vein is a typical Doppler finding at the embryonic time. Placental volume blood flow increased significantly with no change in the placental vascular impedance.

Postprandial response of ghrelin and PYY and indices of low-grade chronic inflammation in lean young women with polycystic ovary syndrome.

The aim of the study were to answer the question 1.) Whether circulating pro-inflammatory markers of endothelial dysfunction and due to chronic low-grade inflammation of obesity, are altered in untreated lean, young relatively healthy polycystic ovary syndrome (PCOS) patients in comparison with healthy controls; 2.) Whether postprandial plasma concentration pattern of ghrelin and PYY can be predictable as risk factors for atherosclerosis and depend of obesity. Forty young women with PCOS were divided in two groups: 19 lean and 21 obese. The control group included 20 lean, healthy volunteers. Plasma total and active ghrelin, total PYY and PYY(3-36), serum adiponectin and insulin were measured using RIA technique, serum sCD40L, visfatin, sP-, sE-selectins, resistin by EIA. Composition of test meal was: 527 kcal total and consisted of 24.1% fat, 54.4% carbohydrate and 21.5% protein. Total and active ghrelin and total PYY were significantly lower in obese PCOS women, whereas active ghrelin was also significantly lower in lean PCOS women compared to controls. Postprandial plasma total ghrelin levels decrease were blunted in lean and obese compared to controls (12.8 % and 18.2% vs 28.2 %). Postprandial plasma active ghrelin decreased in lean and obese PCOS groups (49.9 % and 44.1 %) and controls (63.8 %). PCOS subjects exhibited smaller rises in postprandial levels of total PYY. Postprandial plasma PYY(3-36) levels increased in obese PCOS women (30.9 %) and controls (41%), whereas lean PCOS women exhibited blunted increase (11.5%). sCD40L levels increased, whereas adiponectin decreased in PCOS groups independently, whereas rise in visfatin, sE- and sP-selectin and the fall in adiponectin was associated with obesity. sP- and sE -selectins correlated positively with obesity. In summary, our study provides the first evidence that lean untreated young PCOS women contribute to the so called "pancreatic islet adaptation to insulin resistance" because of ghrelin and PYY profiles. We confirmed existing of low-grade chronic inflammation in early stage of visceral obesity in lean PCOS patients. The lost endogenous "islet adaptation to insulin resistance" may lead to endothelial dysfunction and promote acceleration of atherosclerosis.

Doppler study of the embryonic heart in normal pregnant women.

OBJECTIVE: To describe normal fetal cardiac and hemodynamic development in normal early first trimester pregnancies. MATERIALS AND METHODS: Eighty-eight women with singleton, uncomplicated pregnancies were prospectively studied with transvaginal ultrasound, pulsed and color Doppler. Heart diameter, heart rate, and inflow and outflow waveforms with valve signals were documented. The proportion of the cardiac cycle of isovolumetric relaxation time (IRT%) and isovolumetric contraction time (ICT%) as well as Tei index were calculated. RESULTS: Ninety-one percent of studies were successful. Heart diameter and the fetal heart rate showed a positive correlation with increasing gestational age: R = 0.80 (p < 0.000001), R = 0.76 (p < 0.000001), respectively. Mean heart diameter at 6 weeks was 1.28 +/- 0.26 mm and mean fetal heart rate was 117 +/- 6 bpm compared to 3.88 +/- 0.54 mm and 171 +/- 6 bpm at 10 weeks. The inflow waveform was monophasic (atrial contraction) in all cases from 6 to 9 weeks. Eight pregnancies (9%) miscarried between 6 and 12 weeks of gestation and the heart exams were characterized by increased IRT% compared with the survivors. In survivors, IRT% decreased between 7 and 8 weeks, from 32.9 +/- 10.7% to 20.8 +/- 5.7% (p < 0.0001). ICT% decreased from 18.6 +/- 4.4% of the cardiac cycle at 8 weeks to 12.6 +/- 4.4% at 9 weeks (p < 0.0008) (after heart development period). CONCLUSIONS: Doppler examination of the fetal cardiac function is possible after 5 weeks of gestation. After 8 weeks of gestation, the fetal heart is morphologically mature but has not yet achieved effective myocardial compliance. The embryonic human heart is dependent on the atrial contraction for ventricular filling throughout the period of cardiac development. Non-survivors manifest myocardial dysfunction.

Basal and postprandial gut peptides affecting food intake in lean and obese pregnant women.

Maternal obesity has been reported as a risk factor for various maternal and fetal complications. The aim of the present study was to examine the patterns of basal and postprandial plasma concentrations of certain gut hormones affecting food intake such as acylated ghrelin, peptide YY(3-36) (PYY(3-36)), cholecystokinin (CCK), insulin and glucose in pregnant women with varying body mass gain during physiological pregnancy. The study included 34 women with singleton pregnancies in the 2(nd) trimester of gestation. The examined pregnant women were divided into 4 groups; I. control pregnancy (CP) with weight gain below 0.5 kg/week; II. overweight low weight gain <1 kg/week (OLWG), III. overweight high weight gain >1 kg/week (OHWG); morbidly obese pregnant with weight gain >1.5 kg/week (MOP). The basal acylated-ghrelin levels in MOP subjects were significantly higher than those in CP and no usual suppression of acylated ghrelin after the meal observed in CP as well as in OLWG and OHWG was found in MOP women. Basal PYY(3-36) plasma levels were similar in CP, OLWG and OHWG but in MOP was significantly reduced and no significant increase in hormone level, typically observed in CP, was detected after a meal in overweight or obese women studied. The fasting CCK and C-reactive protein (CRP) levels in MOP subjects were significantly higher than those in CP and other overweight women. In conclusion, we found that pregnant women with overweight and obesity exhibit significant changes in fasting and postprandial gut hormones affecting food intake such as acylated ghrelin, PYY(3-36) and CCK as well as in CRP and these changes might contribute, at least in part, the development of obesity in pregnancy.

Role of leptin, ghrelin, angiotensin II and orexins in 3T3 L1 preadipocyte cells proliferation and oxidative metabolism.

There is now growing evidence that the reactive oxygen species have an influence on proliferation and antioxidative status of various cell types. The aim of the study was to investigate the effects of different concentrations of leptin, ghrelin, angiotensin II and orexins on proliferation, culture medium malondialdehyde (MDA) levels and antioxidative enzymes activities: superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) in 3T3 L1 preadipocytes cell culture. Cell proliferation was measured using [(3)H]tymidine incorporation. In 3T3-L1 cells leptin caused a significant reduction in proliferation (by 36%) compared to control. Ghrelin increased preadipocyte proliferation, and the effect was stronger in higher dose (by 39%), while proproliferatory effect of angiotensin II was stronger in lower doses (by 47%). All used doses of orexin A significantly increased 3T3 L1 cell proliferation (from 21% to 160%), while orexin B caused a marked reduction (from 35% to 70%) of this proliferation. The effects of both orexins were dose-dependent. Leptin and ghrelin increased activity of SOD, CAT, GSH-Px and decreased level of MDA. Angiotensin II treatment stimulated only SOD and CAT activities. Influence of orexins was different on various enzymes. Orexin A increased MDA levels, while orexin B caused a marked decrease in MDA levels. Our results strongly suggest the effects of appetite affecting hormones such as leptin and ghrelin on proliferation and antioxidative enzyme activities of preadipocyte cell lines. Orexin A was found to be the most efficient proliferative-signalling hormone, while orexin B revealed the most significant inhibitory effect on preadipocytes proliferation.

[Endothelin and nitric oxide in newborn kidney]. / Fizjologiczna rola endoteliny i tlenku azotu w nerce noworodka.

The review addresses two of vasoactive substances: endothelin and nitric oxide and describes how each of them effects the functions of endothelial cells of kidneys in newborn.

[Sonographic evaluation of cervical length and cervical canal width in 24th week of twin pregnancy in prediction of preterm delivery]. / Ultrasonograficzny pomiar szyjki macicy w ciazy blizniaczej i jego wartosc w przewidywaniu porodu przedwczesnego.

OBJECTIVE: Sonographic evaluation of the cervical length and cervical canal width in 24th week of twin pregnancy in prediction of preterm delivery. MATERIALS AND METHODS: 43 primiparous patients with twin pregnancy. Group I (n = 16, 37.2%) with spontaneous delivery before 36 of gestation; group II (n = 27, 62.8%) delivering in term. Sonographic measurements of the cervical length and cervical canal width were done in 24th week of gestation. RESULTS: Mean values of cervical length in group I were: 25.6 mm (+/- 3.7 mm); and in group II--32.5 mm (+/- 6.0 mm). Corresponding values of canal width were: 14.6 mm (+/- 11.8 mm) and 6.2 mm (+/- 3.0 mm). CONCLUSIONS: We suggest, that cervical length is more valuable parameter in predicting risk of preterm delivery in twin pregnancy.

[Three dimensional sonography in nuchal translucency measurements between 10th and 14th weeks of gestation]. / Trójwymiarowa ultrasonografia w ocenie grubosci faldu karkowego plodu pomiedzy 10-tym a 14-tym tygodniem ciazy.

Our objective was to determine the practical usefulness of 3D transvaginal sonography in nuchal translucency (NT) measurements between 10th and 14th week of gestation. Thirty-six women (18 primiparas) were studied with the use of Kretz Voluson 530 and 730 scanners (Kretztechnik, Zipf, Austria). Two different volume scans were performed in all subjects according to the new 4 steps method proposed by Chung et al (2000). Nuchal translucency of the first and second scan were compared. In all but one (2.7%) cases good quality images were obtained. Mean value of NT of the first and second scan were 1.463 +/- 0.551 mm (SD) and 1.475 +/- 0.556 mm (SD), respectively. The differences as compared by two tailed t-test for paired measurements were not significant (p = 0.668). There was a significant correlation between first and second measurement (r = 0.96), also the interoperator variability was good. Our preliminary study indicates that 3D sonography is a useful tool in nuchal translucency measurements at the end of the first trimester of pregnancy. The results, however, need to be prospectively tested in larger population.

[Sonographic evaluation of the uterus on 3rd day after normal, vaginal delivery in women with benign fever]. / Ultrasonograficzna ocena macicy we wczesnym okresie poporodowym.

OBJECTIVE: Sonographic evaluation of the uterus on 3rd day after normal, vaginal delivery in women with benign fever. MATERIALS AND METHODS: 112 primiparous patients after vaginal delivery on term, following uncomplicated pregnancy. Group I (n = 78, 69.6%) with body temperature below 37.5 degrees C, group II (n = 34, 30.4%) with temperature higher than 37.5 degrees C. In all patients following sonographic features were evaluated: uterus volume, uterine cavity volume and uterine cavity content. RESULTS: No significant differences were observed. CONCLUSIONS: We suggest, that uterus volume, uterine cavity volume and uterine cavity content is not often correlated with benign fever following vaginal delivery.

[Nuchal cord and nuchal translucency measurements between 10th and 14th weeks of gestation]. / Wplyw obecnosci pepowiny na szyi plodu na pomiar grubosci faldu karkowego pomiedzy 10-tym a 14-tym tygodniem ciazy.

Our objective was to determine the incidence of nuchal cord and it's possible influence on the nuchal translucency (NT) measurements between 10th and 14th week of gestation. One hundred fourteen singleton pregnancies were studied with transvaginal sonography. Nuchal translucency was measured according to the criteria published by Snijders et al. from the Fetal Medicine Foundation. Nuchal cord was detected with color Doppler flow mapping in 8 of 114 fetuses (7.01%). Mean cord thickness in these fetuses was 1.1 mm (range 0.5-1.4 mm). Mean nuchal translucency with and without umbilical cord were 1.67 mm (SD = 0.67; range: 0.5-3.6) and 1.59 mm (SD = 0.54; range: 0.5-3.0), respectively. The differences were statistically significant (p = 0.0146, Student's t-test for paired observations). We conclude that although relatively infrequent, nuchal cord might have influence on nuchal translucency measurements at the end of the Ist trimester.

[Doppler evaluation as a predictor of asphyxia in fetuses with intrauterine growth retardation (IUGR)]. / Dopplerowskie cechy centralizacji krazenia we wczesnej ocenie niedotlenienia u plodów z wewnatrzmacicznym zahamowaniem wzrostu (IUGR).

Many papers showed that the pulsatility index of the fetal middle cerebral artery decreases during gestation. We observed equalization of the PI in MCA and PI in UMB in the perinatal period (after 38 weeks of gestation). We observed the increase the diastolic cerebral flow through the brain in cases of hypoxia. We interpreted that situation as a vasomotor response to fetal hypoxia (for example in IUGR). This phenomenon, is called in the literature as brain sparing effect. The aim of our study was estimation of application Doppler technique in diagnosis of changes in circulatory system in pregnancies complicated by intrauterine growth restriction (IUGR). We included 66 fetuses with IUGR and sonographic signs of the brain sparing effects, without any congenital malformation, oedema and genetic abnormalities to the study group. We included 1730 foetuses from normal, physiological pregnancies to the control group. In all cases time of gestation were calculated from Naegele's rule and were confirmed in sonographic examination before 16 weeks of gestation. SGA fetuses were defined on the basis of ultrasonographic measurements of BPD, HC, AC, FL and weight below 10th percentile of our reference curve. Sonographic examination were performed on Acuson 128 XP/10 with transducers (2.5 MHz-5 MHz). We performed Doppler examinations in MCA and UMB. Redistribution (brain sparing effect) was diagnosed when CPR (cerebroplacental ratio) was less than 1. We analysed the way and time of delivery and neoanatal follow up. Obtained in advance of researches results made possible expression of following conclusions: 1. Use of Doppler techniques in pregnancies complicated by IUGR makes possible diagnosis of hypoxia that helps in settlement of continuing or ending of pregnancy. 2. Changes in Doppler flows in foetus resoluteness outdistance irregular other values of biophysical methods of foetus monitoring.

Fetal functional pulmonary atresia with ascites resolving spontaneously before birth. A case report.

In this case we report functional pulmonary atresia in a fetus with significant tricuspid valve regurgitation and severe ascites, with spontaneous improvement without prenatal intervention and a subsequent follow-up at 8 weeks after birth.