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1.
Vet Parasitol ; 193(1-3): 100-4, 2013 Mar 31.
Article in English | MEDLINE | ID: mdl-23200751

ABSTRACT

There are currently no reports on the isolation and molecular examination of Toxoplasma gondii from bats. Here, we report the isolation and genotypic characterisation of two T. gondii isolates from bats. A total of 369 bats from different municipalities in São Paulo state, southeastern Brazil, were captured and euthanised, and collected tissues (heart and pectoral muscle) were processed for each bat or in pools of two or three bats and bioassayed in mice (a total of 283 bioassays). Eleven PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) markers were used to genotype positive samples: SAG1, SAG2 (5'-3'SAG2 and alt. SAG2), SAG3, BTUB, GRA6, L358, c22-8, c29-2, PK1, CS3 and Apico. The parasite was isolated from two bats from São Paulo city: an insectivorous bat, the velvety free-tailed bat Molossus molossus, and a hematophagous bat, the common vampire bat Desmodus rotundus. Isolates were designated TgBatBr1 and TgBatBr2, respectively. The genotype of the isolate from M. molossus (TgBatBr1) has been previously described in an isolate from a capybara from São Paulo state, and the genotype from the D. rotundus isolate (TgBatBr2) has already been identified in isolates from cats, chickens, capybaras, sheep, a rodent and a common rabbit from different Brazilian states, suggesting that this may be a common T. gondii lineage circulating in some Brazilian regions. Isolation of T. gondii from a hematophagous species is striking. This study reveals that bats can share the same isolates that are found in domesticated and wild terrestrial animals. This is the first report of the isolation and genotyping of T. gondii in chiropterans.


Subject(s)
Chiroptera/parasitology , Genotype , Toxoplasma/genetics , Toxoplasma/isolation & purification , Toxoplasmosis, Animal/parasitology , Animals , Brazil/epidemiology , Polymerase Chain Reaction/methods , Polymerase Chain Reaction/veterinary , Polymorphism, Restriction Fragment Length , Toxoplasmosis, Animal/epidemiology
3.
São Paulo; SMS; 2011. 1 p. ilus, tab, graf.
Non-conventional in Portuguese | Sec. Munic. Saúde SP, COVISA-Producao, Sec. Munic. Saúde SP, Sec. Munic. Saúde SP | ID: sms-2540
5.
São Paulo; Secretaria da Saúde. Coordenação de Vigilância em Saúde. Gerência do Centro de Controle de Zoonoses. Gerência de Vigilância em Saúde Ambiental; 2011. 1 p. ilus, map, tab.
Non-conventional in Portuguese | Coleciona SUS, COVISA-Producao, Sec. Munic. Saúde SP, Sec. Munic. Saúde SP | ID: biblio-937374
6.
São Paulo; Secretaria Municipal da Saúde. Coordenação de Vigilância em Saúde; 2011. 1 p. ilus, tab, graf.
Non-conventional in Portuguese | Coleciona SUS, COVISA-Producao, Sec. Munic. Saúde SP, Sec. Munic. Saúde SP | ID: biblio-937451
7.
São Paulo; Secretaria Municipal da Saúde. Coordenação de Vigilância em Saúde; 2011. 1 p. ilus.
Non-conventional in Portuguese | Coleciona SUS, COVISA-Producao, Sec. Munic. Saúde SP, Sec. Munic. Saúde SP | ID: biblio-937457
8.
Article in English | LILACS | ID: lil-587783

ABSTRACT

Current knowledge on bat lyssavirus infections in their native hosts is limited and little is known about the virulence, virus dissemination and transmission among free-living insectivorous bats. The present study is a brief description of rabies virus (RABV) dissemination in tissues of a naturally infected pregnant southern yellow bat (Lasiurus ega) and its fetuses, obtained by reverse-transcriptase polymerase chain reaction (RT-PCR). The RT-PCR was positive in samples from the brain, salivary gland, tongue, lungs, heart, kidneys and liver. On the other hand, the placenta, three fetuses, spleen, intestine and brown fat tissue tested negative. This research demonstrated the absence of rabies virus in the fetuses, thus, in this specific case, the transplacentary transmission was not observed.


Subject(s)
Animals , Rabbits , Chiroptera , Rabies , Rabies virus , Polymerase Chain Reaction/methods , Infectious Disease Transmission, Vertical/veterinary , Rabbits
9.
São Paulo; SMS; 2010. 1 p. ilus, tab.
Non-conventional in Portuguese | Sec. Munic. Saúde SP, COVISA-Producao, Sec. Munic. Saúde SP, Sec. Munic. Saúde SP | ID: sms-7723
10.
São Paulo; SMS; 2010. 1 p. ilus, tab.
Non-conventional in Portuguese | Coleciona SUS, COVISA-Producao, Sec. Munic. Saúde SP, Sec. Munic. Saúde SP | ID: biblio-939568
12.
São Paulo; Instituto Pasteur;CDC;Instituto Biólogico;SMS;UNESP; 2000. 2 p.
Non-conventional in Portuguese | Coleciona SUS, COVISA-Producao, Sec. Munic. Saúde SP, Sec. Munic. Saúde SP | ID: biblio-938026
13.
São Paulo; Instituto Pasteur;SMS; 2000. 1 p.
Non-conventional in Portuguese | Coleciona SUS, COVISA-Producao, Sec. Munic. Saúde SP, Sec. Munic. Saúde SP | ID: biblio-938034
14.
São Paulo; Instituto Pasteur;SMS; 2000. 1 p.
Non-conventional in Portuguese | Sec. Munic. Saúde SP, COVISA-Producao, Sec. Munic. Saúde SP, Sec. Munic. Saúde SP | ID: sms-4380
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