Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 2 de 2
Filter
Add more filters










Database
Language
Publication year range
1.
Dent Res J (Isfahan) ; 9(3): 256-60, 2012 May.
Article in English | MEDLINE | ID: mdl-23087728

ABSTRACT

BACKGROUND: Fungi may play a key part in periradicular diseases. The aim of this study was to evaluate and compare the antifungal properties of two root-end filling materials, ProRoot Mineral trioxide aggregate (MTA) and MTA-Angelus, against Candida albicans using tube-dilution test. MATERIALS AND METHODS: The antifungal properties of ProRoot MTA and MTA-Angelus against C. albicans was assessed at 1, 24, and 48 hours following administration of two concentrations of the antifungal agents (50 and 100 mg/ml). A total of 50 culture wells were divided into four experimental groups (Freshly mixed MTA, Freshly mixed MTA-Angelus, 24-h set MTA, and 24-h set MTA-Angelus) and two control groups. Each well was prepared for one specific agent with a specific concentration. For the set groups, the mixture was prepared and left for 24 hours. One milliliter of suspension of fungal colonies with concentration of 10(4) CFU/ml was then added to the mixtures in each well. All wells were incubated at 37°C and assessed at 1, 24, and 48 hours. This observation was based on the turbidity of the suspension in the tubes. At each time point, 0.02 ml of each suspension was cultured on a Sabouraud dextrose agar plate to confirm C. albicans growth. The results were analyzed using Kruskal-Wallis test. RESULTS: Although all fresh and set samples were incapable of killing C. albicans at 1 hour, they demonstrated fungicidal ability on agar plates at 24 and 48-hour time points. CONCLUSION: MTA-angelus proved to be an effective antifungal agent compared to ProRoot MTA at concentrations of 50 mg/ml and 100 mg/ml.

2.
Iran Endod J ; 4(3): 101-5, 2009.
Article in English | MEDLINE | ID: mdl-24003330

ABSTRACT

INTRODUCTION: The purpose of this ex vivo study was to assess the effect of two root-end filling materials against Candida (C) Albicans. MATERIALS AND METHODS: ProRoot MTA and CEM Cement were compared immediately and 24 h after mixing, in two different concentrations (50 and 100 mg/mL). A total of 50 culture wells were used and divided into experimental (n=10) and control groups (n=5). Those with no medication served as positive and without C. Albicans served as negative controls. All plates were incubated at 37(°)C after 1, 24, and 48hours. At each interval, the presence of C. Albicans was assessed and recorded by an independent observer. In addition to observing turbidity, 0.02 mL of samples from each cell was re-cultured on sabouraud dextrose agar plates to confirm change in fungal growth. The data were evaluated and analyzed using Kruskal-Wallis test. RESULTS: Although all fresh and set samples with experimental concentrations showed fungal growth after 1 h; they demonstrated complete fungicidal activity at 24 and 48-h time intervals. CONCLUSION: Under the conditions of this ex vivo study, CEM cement as well as ProRoot MTA has fungicidal effects against C. Albicans even in concentration of 50 mg/mL and after 24 hours.

SELECTION OF CITATIONS
SEARCH DETAIL
...