Effects of food-simulating liquids on surface properties of giomer restoratives.

This study examined the effects of food-simulating liquid (FSL) on the hardness and roughness of giomer restoratives based on pre-reacted glass ionomer (PRG) technology. The materials investigated included a regular (Beautifil II [BT]) and a recently introduced injectable (Beautifil Flow Plus F00 [BF]) hybrid PRG composite. A direct hybrid composite (Filtek Z250 [ZT]) and an indirect hybrid composite (Ceramage [CM]) were used for comparison. The materials were placed into customized square molds (5 mm × 5 mm × 2.5 mm), covered with Mylar strips, and cured according to manufacturers' instructions. The materials were then conditioned in air (control), distilled water, 50% ethanol solution, and 0.02 N citric acid at 37°C for seven days. Specimens (n=6) were then subjected to hardness testing (Knoop) and surface profilometry. Data were analyzed using one-way analysis of variance and post hoc Scheffe test (p<0.05). Mean Knoop hardness values for the control group (air) ranged from 53.4 ± 3.4 (BF) to 89.5 ± 5.2 (ZT), while mean surface roughness values values ranged from 0.014 ± 0.002 (ZT) to 0.032 ± 0.001 (BT). All materials were significantly softened by FSL. The degree of softening by the different FSLs was material dependent. The hardness of giomers was most affected by citric acid and ethanol. The smoothest surface was generally observed with the control group. Giomer restoratives were significantly roughened by citric acid.

Effectiveness of composite cure associated with different light-curing regimes.

This study investigated the use of various light-curing regimens with standardized light energy density on the effectiveness of cure of a visible light activated resin composite (Z100, 3M-ESPE). A light-cure unit (Variable Intensity Polymerizer (VIP), BISCO Inc) which permitted individual control over time and intensity, was used. The five light-curing modes investigated include Pulse Delay (PD), Pulse Cure (PC), Soft-start (SS), Turbo (T) and Control (C). Effectiveness of cure was established by measuring the top and bottom Knoop hardness of 2-mm thick composite specimens using a digital microhardness tester (n=5, load=500g; dwell time=15 seconds) immediately and at one-day post-polymerization. Data obtained was analyzed using one-way ANOVA/Scheffe's post hoc test and Independent Samples t-tests (p<0.05). Top KHN observed immediately after polymerization with C was significantly lower than PD. At one day post-polymerization, the top KHN obtained with C was significantly lower than PD, SS and T. No significant difference in bottom KHN was observed among the different curing modes immediately after curing. At one day post-polymerization, the bottom KHN obtained with C was significantly lower than SS and T. Regardless of curing regimens, top and bottom values at one day were significantly higher than those observed immediately after light polymerization. No significant difference in mean hardness ratio was observed among the different curing regimens immediately and one day later. Effectiveness of the cure at the bottom surfaces of composites may be increased by soft-start and turbo polymerization regimens.

Curing efficacy of a new generation high-power LED lamp.

This study investigated the curing efficacy of a new generation high-power LED lamp (Elipar Freelight 2 [N] 3M-ESPE). The effectiveness of composite cure with this new lamp was compared to conventional LED/halogen (Elipar Freelight [F], 3M-ESPE; Max [M], Dentsply-Caulk) and high-power halogen (Elipar Trilight [T], 3M-ESPE; Astralis 10 [A], Ivoclar Vivadent) lamps. Standard continuous (NS, FS, TS; MS), turbo (AT) and exponential (NE, FE, TE) curing modes of the various lights were examined. Curing efficacy of the various lights and modes were determined by measuring the top and bottom surface hardness of 2-mm thick composite specimens (Z100, 3M-ESPE) using a digital microhardness tester (n=5; load=500 g; dwell time=15 seconds) one hour after light polymerization. The hardness ratio was computed by dividing HK (Knoops Hardness) of the bottom surface by HK of the top surface. The data was analyzed using one-way ANOVA/Scheffe's test and Independent Samples t-test at significance level 0.05. Results of the statistical analysis were as follows: HK top--E, FE, NE > NS and NE > AT, TS, FS; HK bottom--TE, NE > NS; Hardness ratio--NS > FE and FS, TS > NE. No significant difference in HK bottom and hardness ratio was observed between the two modes of Freelight 2 and Max. Freelight 2 cured composites as effectively as conventional LED/halogen and high-power halogen lamps, even with a 50% reduction in cure time. The exponential modes of Freelight 2, Freelight and Trilight appear to be more effective than their respective standard modes.

Post-gel polymerization shrinkage associated with different light curing regimens.

This study compared post-gel polymerization shrinkage associated with five different light curing regimens of similar light energy density. A light-cure unit (VIP, BISCO) that allowed for independent command over time and intensity was used. The five regimens investigated were pulse delay (PD), soft-start (SS); pulse cure (PC), turbo cure (TC) and standard continuous cure (C) [control]. With the exception of TC, the light energy density for all curing regimens was fixed at 16 J/cm2. A strain-monitoring device and test configuration were used to measure the linear polymerization shrinkage of 2-mm thick composite specimens (Z100, 3M ESPE) during and post-light polymerization up to 60 minutes. Five samples were made for each curing mode. The results were analyzed using ANOVA/Scheffee's post-hoc test at significance level 0.05. Post-gel shrinkage ranged from 0.30% to 0.46 % at 60 minutes. The use of PD resulted in significantly lower shrinkage compared to PC, TC, SS and C. Shrinkage associated with SS was, in general, significantly lower than C. No significant difference in shrinkage was observed between PC, TC and C at all time intervals. The use of pulse delay and soft-start regimens decreased post-gel polymerization shrinkage.

Analysis of the degree of conversion of LED and halogen lights using micro-Raman spectroscopy.

This study determined the degree of conversion of two LED (light-emitting diodes) (Elipar FreeLight [FL], 3M ESPE; GC e-Light [EL], GC), a high intensity (Elipar TriLight [TL], 3M ESPE) and a very high intensity (Astralis 10 [AS], Ivoclar Vivadent) halogen light. The degree of conversion of these lights was compared to a conventional halogen light (Max [MX] (control), Dentsply-Caulk). Ten different light curing regimens, including pulse (EL1), continuous (FL1, EL2, TL1), turbo (EL3, AS1) and soft-start (FL2, EL4, TL2) modes of various lights were also investigated. Composite specimens of dimensions 3 x 3 x 2 mm were cured with the 10 different light curing regimens investigated. Micro-Raman spectroscopy was used to determine the degree of conversion at the top and bottom surfaces of a composite restorative (Z100, [3M ESPE]) at 60 minutes post-light polymerization. Five specimens were made for each cure mode. The results were analyzed using ANOVA/Scheffe's post-hoc test and Independent Samples t-tests at significance level 0.05. The degree of conversion ranged from 55.98 +/- 2.50 to 59.00 +/- 2.76% for the top surface and 51.90 +/- 3.36 to 57.28 +/- 1.56% for the bottom surface. No significant difference in degree of conversion was observed for the 10 light curing regimens when compared to MX (control). The curing efficiency of LED lights was comparable to halogen lights regardless of curing modes.

Influence of curing lights and modes on cross-link density of dental composites.

This study investigated the influence of curing lights and modes on the cross-link density of dental composites. Four LED/halogen curing lights (LED-Elipar Freelight [FL], 3M-ESPE and GC e-light [EL], GC; high intensity halogen-Elipar Trilight [TL], 3M-ESPE; very high intensity halogen-Astralis 10 [AS], Ivoclar Vivadent) were selected for this study. Pulse (EL1), continuous (FL1, EL2, TL1), turbo (EL3, AS) and soft-start (FL2, EL4, TL2) curing modes of the various lights were examined. A conventional, continuous cure halogen light (Max [MX], Dentsply-Caulk) was used for comparison. Six composite (Z100, 3M-ESPE) specimens were made for each light-curing mode combination. After polymerization, the specimens were stored in air at 37 degrees C for 24 hours and subjected to hardness testing using a digital microhardness tester (load=500 g; dwell time=15 seconds). The specimens were then placed in 75% ethanol-water solution at 37 degrees C for 24 hours and post-conditioning hardness was determined. Mean hardness (HK)/change in hardness (deltaHK) was computed and the data subjected to analysis using one-way ANOVA/Scheffe's test and Independent Samples t-test (p<0.05). Softening upon storage in ethanol (deltaHK) was used as a relative indication of cross-link density. Specimens polymerized with AS, TL2 and all modes of both LED lights were significantly more susceptible to softening in ethanol than specimens cured with MX. No significant difference in cross-link density was observed among the various modes of EL and FL. For TL, curing with continuous mode resulted in specimens with significantly higher cross-link density than curing with the soft-start mode.

Elution of leachable components from composites after LED and halogen light irradiation.

This study investigated the influence of curing lights and modes on the elution of leachable components from dental composites. Four LED/halogen curing lights (LED-Elipar Freelight [FL], 3M-ESPE and GC e-light [EL], GC; high intensity halogen-Elipar Trilight [TL], 3M-ESPE; very high intensity halogen-Astralis 10 [AS], Ivoclar Vivadent) were selected for this study. Pulse (EL1), continuous (FL1, EL2, TL1), turbo (EL3, AS) and soft-start (FL2, EL4, TL2) curing modes of the various lights were examined. A conventional continuous cure halogen light (Max [MX], Dentsply-Caulk) was used for comparison. Three composite (Z100, 3M-ESPE) specimens (6.5 mm in diameter and 1-mm thick) were made for each curing light-mode combination. After polymerization, the specimens were stored in air at 37 degrees C for 24 hours and incubated in acetonitrile at 37 degrees C for 24 hours. BisGMA and TEGDMA extracts were isolated by high performance liquid chromatography (HPLC). Data were subjected to analysis using one-way ANOVA/Scheffe's post-hoc test and Independent Samples t-test at significance level 0.05. The total monomer (BisGMA and TEGDMA) eluted ranged from 8.75 to 27.97 ppm for FL1 and AS, respectively. Significantly more unreacted monomers were leached from composites cured with all modes of EL and AS when compared to MX. No significant difference in the total monomer eluted was observed between the two modes of FL/TL and MX Although composites cured with EL2 released significantly less monomer than EL1, 3 and 4, no significant difference in the total monomer eluted was observed between the continuous and soft-start modes of FL and TL. The elution of leachable components from composites appears to be curing light specific rather than light source (LED or halogen) and curing mode specific.

Post-gel shrinkage with different modes of LED and halogen light curing units.

This study compared the post-gel shrinkage of two LED (light-emitting diodes) lights (Elipar FreeLight [FL], 3M ESPE; GC e-Light [EL], GC), a high intensity (Elipar TriLight [TL], 3M ESPE) and a very high intensity (Astralis 10 [AS], Ivoclar Vivadent) halogen light to a conventional (Max [MX] (control), Dentsply-Caulk) halogen light. Ten light curing regimens were investigated. These included continuous (FL1, EL2, MX, TL1 and AS1), soft-start (FL2, EL4, TL2), pulse activation (EL1) and turbo (EL3) modes. A strain-monitoring device and test configuration was used to measure the linear polymerization shrinkage of a composite restorative (Z100, [3M ESPE]) during and post-light polymerization up to 60 minutes when cured with the different modes. Five specimens were made for each cure mode. Results were analyzed using ANOVA/Scheffe's post-hoc test and independent sample t-tests at significance level 0.05. Shrinkage associated with the various modes of EL was significantly lower than MX immediately after light polymerization and at one-minute post-light polymerization. No significant difference between MX and the various lights/cure modes was observed at 10, 30 and 60-minutes post-light polymerization. At all time intervals, post-gel shrinkage associated with continuous light curing mode was significantly higher than the soft-start light curing mode for FL and TL.

Influence of curing modes on crosslink density in polymer structures.

OBJECTIVE: This study investigates the influence of curing modes on the crosslinking density of dental composites. METHODS: A light-cure unit (BISCO VIP) that allowed for independent command over time and intensity was selected. Four different light-curing modes with constant light energy density were investigated (control (C), pulse delay (PD), soft-start (SS) and pulse cure (PC)). The degree of crosslinking was assessed directly by measuring the glass transition temperature of 1 mm thick composite (Z100, 3M-ESPE) specimens using differential scanning calorimetry (DSC 2920). Polymer softening in ethanol was used as an indirect method for assessing the degree of crosslinking. After light-curing, specimens were stored in air at 37 degrees C for 24 h and subjected to hardness testing using a digital microhardness tester (n = 6, load=500 g; dwell time=15 s). The specimens were then placed in 75% ethanol-water solution at 37 degrees C for 24 h and post-conditioning hardness was determined. Mean hardness (KHN)/hardness deterioration (DeltaKHN) was computed and data was subjected to analysis using one-way ANOVA/Scheffe's test. RESULTS: Ranking of degree of crosslinking density by DSC was as follows: C>PC>SS>PD. For the indirect method of determining crosslinking density, DeltaKHN ranged from 10.8 to 12.9 and ranking was PC>SS>C>PD. CONCLUSIONS: Specimens polymerized with PD were significantly more susceptible to softening in ethanol than specimens cured with PC. Results of this study suggest that polymerization with PD resulted in a lower crosslink density and gave rise to polymers with an increased susceptibility to softening in ethanol.

Post-gel polymerization contraction of "low shrinkage" composite restoratives.

This study compared the post-gel contraction of two "low-shrinkage" composites (InTen-S [IS], Ivoclar-Vivadent; Aelite LS [AL], BISCO Inc) and an ormocer (Admira [AM], Voco) to two conventional mini-filled composites (Renew [RN], BISCO; Z100 [ZO], 3M ESPE). A strain-monitoring device and test configuration were used to measure the linear polymerization shrinkage associated with the various composites (A2 shade) during and up to 60 minutes post light polymerization. Each specimen was irradiated for 40 seconds using a halogen curing light (Max, Dentsply-Caulk) with an intensity of 401 mW/cm2. Five specimens were made for each composite. Data was analyzed using one-way ANOVA/Scheffe's post-hoc test at significance level 0.05. The linear percentage shrinkage immediately after light polymerization and at 60 minutes post light polymerization ranged from 0.10 +/- 0.02 to 0.40 +/- 0.02% and 0.22 +/- 0.02 to 0.60 +/- 0.05%, respectively. Post-gel shrinkage ranking of the materials was as follows: immediately after light polymerization - IS < AL < AM < ZO < RN and at 60 minutes post light polymerization - IS < AL = AM < ZO < RN. The shrinkage associated with IS, AL and AM was significantly lower than for ZO and RN immediately after light polymerization and at 1, 10, 30 and 60 minutes post light polymerization. The post-gel polymerization shrinkage of IS, AL and AM was significantly lower than conventional mini-filled composites.

Comparative depths of cure among various curing light types and methods.

This study evaluated the depth of cure associated with commercial LEDs (light-emitting diodes) (Elipar FreeLight [FL], 3M-ESPE; GC e-Light [EL], GC), high intensity (Elipar TriLight [TL], 3M-ESPE) and very high intensity (Astralis 10 [AS], Ivoclar Vivadent) Quartz Tungsten Halogen (QTH) curing lights. Depth of cure of the various lights/curing modes were compared to a conventional QTH light (Max [Mx], Dentsply-Caulk). Ten exposure regimens were investigated: FL1 - 400 mW/cm2 [40 seconds]; FL2 - 0-400 mW/cm2 [12 seconds] --> 400 mW/cm2 [28 seconds]; EL1 - 750 mW/cm2 [10 pulses x 2 seconds], EL2 - 350 mW/cm2 [40 seconds]; EL3 - 600 mW/cm2 [20 seconds]; EL4 - 0 - 600 mW/cm2 [20 seconds] --> 600 mW/cm2 [20 seconds]; TL1 - 800 mW/cm2 [40 seconds]; TL2 - 100- 800 mW/cm2 [15 seconds] --> 800 mW/cm2 [25 seconds]; AS1 - 1200 mW/cm2 [10 seconds]; MX - 400 mW/cm2 [40 seconds]. Depth of cure was determined by penetration, scraping and microhardness techniques. The results were analyzed using one-way ANOVA/Scheffe's post-hoc test and Pearson's correlation at significance level 0.05 and 0.01, respectively. All light curing regimens met the ISO depth of cure requirement of 1.5 mm with the exception of EL1-EL3 with the microhardness technique. Curing with most modes of EL resulted in significantly lower depths of cure than the control [MX]. No significant difference in depth of cure was observed among the control and the two modes of FL. Curing with TL1 resulted in significantly greater depth of cure compared to MX with all testing techniques. No significant difference in depth of cure was observed between the control and AS1 for all testing techniques except for the penetration technique. The depth of composite cure is light unit and exposure mode dependent. Scraping and penetration techniques were found to correlate well but tend to overestimate depth of cure compared to microhardness.

The effectiveness of cure of LED and halogen curing lights at varying cavity depths.

This study compared the effectiveness of cure of two LED (light-emitting diodes) lights (Elipar FreeLight [FL], 3M-ESPE and GC e-Light [EL], GC) to conventional (Max [MX] (control), Dentsply-Caulk), high intensity (Elipar TriLight [TL], 3M-ESPE) and very high intensity (Astralis 10 [AS], Ivoclar Vivadent) halogen lights at varying cavity depths. Ten light curing regimens were investigated. They include: FL1-400 mW/cm2 [40 seconds], FL2-0-400 mW/cm2 [12 seconds] --> 400 mW/cm2 [28 seconds], EL1-750 mW/cm2 [10 pulses x 2 seconds], EL2-350 mW/cm2 [40 seconds], EL3-600 mW/cm2 [20 seconds], EL4-0-600 mW/cm2 [20 seconds] --> 600 mW/cm2 [20 seconds], TL1-800 mW/cm2 [40 seconds], TL2-100-800 mW/cm2 [15 seconds] --> 800 mW/cm2 [25 seconds], AS1-1200 mW/cm2 [10 seconds], MX-400 mW/cm2 [40 seconds]. The effectiveness of cure of the different modes was determined by measuring the top and bottom surface hardness (KHN) of 2-mm, 3-mm and 4-mm thick composite (Z100, [3M-ESPE]) specimens using a digital microhardness tester (n = 5, load = 500 g; dwell time = 15 seconds). Results were analyzed using ANOVA/Scheffe's post-hoc test and Independent Samples t-Test (p < 0.05). For all lights, effectiveness of cure was found to decrease with increased cavity depths. The mean hardness ratio for all curing lights at a depth of 2 mm was found to be greater than 0.80 (the accepted minimum standard). At 3 mm, all halogen lights produced a hardness ratio greater than 0.80 but some LED light regimens did not; and at a depth of 4 mm, the mean hardness ratio observed with all curing lights was less than 0.80. Significant differences in top and bottom KHN values were observed among different curing regimens for the same light and between LED and halogen lights. While curing with most modes of EL resulted in significantly lower top and bottom KHN values than the control (MX) at all depths, the standard mode of FL resulted in significantly higher top and bottom KHN at a depth of 3 mm and 4 mm. The depth of composite cure with LED LCUs was, therefore, product and mode dependent.

Effectiveness of composite cure associated with different curing modes of LED lights.

This study compared the effectiveness of cure of two LED (light-emitting diodes) lights (Elipar FreeLight [FL], 3M-ESPE; GC e-Light [EL], GC) to conventional (Max [MX], Dentsply-Caulk [control]), high intensity (Elipar TriLight [TL], 3M-ESPE) and very high intensity (Astralis 10 [AS], Ivoclar Vivadent) halogen lights. The 10 light-curing regimens investigated were: FL1 400 mW/cm2 [40 seconds], FL2 0-400 mW/cm2 [12 seconds] --> 400 mW/cm2 [28 seconds], EL1 750 mW/cm2 [10 pulses x 2 seconds], EL2 350 mW/cm2 [40 seconds], EL3 600 mW/cm2 [20 seconds], EL4 0-600 mW/cm2 [20 seconds] --> 600 mW/cm2 [20 seconds], TL1 800 mW/cm2 [40 seconds], TL2 100-800 mW/cm2 [15 seconds] --> 800 mW/cm2 [25 seconds], AS1 1200 mW/cm2 [10 seconds], MX 400 mW/cm2 [40 seconds]. Effectiveness of cure with the different modes was determined by measuring the top and bottom surface hardness (KHN) of 2-mm thick composite (Z100, [3M-ESPE]) specimens using a digital microhardness tester (n=5, load=500 g; dwell time=15 seconds). Results were analyzed using one-way ANOVA/Scheffe's post-hoc test and Independent Samples t-test (p<0.05). At the top surface, the mean KHN observed with LED lights ranged from 55.42 +/- 1.47 to 68.54 +/- 1.46, while that of halogen lights was 62.64 +/- 1.87 to 73.14 +/- 0.97. At the bottom surface, the mean KHN observed with LED and halogen lights ranged from 46.90 +/- 1.73 to 66.46 +/- 1.18 and 62.26 +/- 1.93 to 70.50 +/- 0.87, respectively. Significant differences in top and bottom KHN values were observed between different curing regimens for the same light, and between LED and halogen lights. Although curing with most modes of EL resulted in significantly lower top and bottom KHN values than the control, no significant difference was observed for the different modes of FL. Hence, the effectiveness of composite cure with LED LCUs is product dependent.

Thermal emission by different light-curing units.

This study quantified and compared the thermal emission of different light curing units (LCU). Three LED (Elipar Freelight [3M]; GC e-light [GC]; Coolblu [Dentalsystems.com]) and three halogen (Max [Dentsply-Caulk]; Elipar Trilight [3M]; Astralis 10 [Ivoclar-Vivadent]) lights were selected for the study. Thermal emission of the LCUs, when used in various curing modes, was assessed using a K-type thermocouple and a digital thermometer at distances of 3 mm and 6 mm compared to the conventional halogen LCU (Max). The temperature profiles and mean maximum temperature change (n = 7) generated by each LCU were obtained. Data was subjected to ANOVA/Scheffe's post-hoc test and Independent Samples t-test at significance level 0.05. At 3 mm, temperature rise observed with LED lights ranged from 4.1 degrees C to 12.9 degrees C, while halogen lights ranged from 17.4 degrees C to 46.4 degrees C. At 6 mm, temperature rise ranged from 2.4 degrees C to 7.5 degrees C and 12.7 degrees C to 25.5 degrees C for LED and halogen lights, respectively. Thermal emission of LED lights was significantly lower than halogen lights. Significant differences in temperature rise were observed between different curing modes for the same light and between different LED/halogen lights.

Effectiveness of composite cure with pulse activation and soft-start polymerization.

The study investigated the effectiveness of composite cure with pulse activation and soft-start polymerization. A light-cure unit (BISCO VIP, BISCO Dental Products, Schaumburg, IL 60193, USA) that allowed for independent command over time and intensity was used. The six light-curing modes examined were: Control (C)-400 mW/cm2 [40 seconds]; Pulse Delay I (PDI) -100 mW/cm2 [3 seconds] --> delay [3 minutes] --> 500 mW/cm2 [30 seconds]; Pulse Delay II (PDII) - 200 mW/cm2 [20 seconds] --> delay [3 minutes] --> 500 mW/cm2 [30 seconds]; Soft-start (SS) - 200 mW/cm2 [10 seconds] --> 600 mW/cm2 [30 seconds]; Pulse Cure I (PCI) - 400 mW/cm2 [10 seconds] --> delay [10 seconds] --> 400 mW/cm2 [10 seconds] --> delay [10 seconds] --> 400 mW/cm2 [20 seconds]; and Pulse Cure II (PCII) - 400 mW/cm2 [20 seconds] --> delay [20 seconds] --> 400 mW/cm2 [20 seconds]. Effectiveness of cure with the different modes was determined by measuring the top and bottom surface hardness of 2 mm thick composite (Z100) specimens using a digital microhardness tester (load=500 gf; dwell time=15 seconds). The effectiveness of cure of the bottom surface of the composite was also established by Fourier Transform Infrared (FTIR) spectroscopy using the KBr technique. Data obtained was analyzed using one-way ANOVA/Scheffe's post-hoc test (p<0.05). No significant difference in top Knoops Hardness Number KHN wa s observed except for PDIand PDII. At the bottom surfaces, KHN obtained with the control was significantly greater than with PDII, SS and PCII. FTIR results ranked well with the hardness of the bottom surfaces. The absorbance ratio of carbon double bonds to aromatic ring obtained with the control group was significantly greater than with PDII and PCII. Effectiveness of the cure at the bottom surfaces of composites may be reduced by some pulse activation and soft-start polymerization regimens.

Post-gel shrinkage with pulse activation and soft-start polymerization.

This study investigated the influence of pulse activation and soft-start polymerization regimens on the post-gel shrinkage of a visible light-activated composite resin (Z100). A light-cure unit (BISCO VIP) that allowed for independent command over time and intensity was used. The six light-curing modes that were examined include: Control (C)-400 mW/cm2 [40 seconds]; Pulse Delay I (PDI)-100 mW/cm2 [3 seconds], delay [3 minutes], 500 mW/cm2 [30 seconds]; Pulse Delay II (PDII)-200 mW/cm2 [20 seconds], delay [3 minutes], 500 mW/cm2 [30 seconds]; Soft-start (SS)-200 mW/cm2 [10 seconds], 600 mW/cm2 [30 seconds]; Pulse Cure I (PCI)--two 400 mW/cm2 [10 seconds] and one 400 mW/cm2 [20 seconds] pulses with 10 seconds interval between; and Pulse Cure II (PCII)-two 400 mW/cm2 [20 seconds] pulses with 20 seconds interval between. A strain-monitoring device measured the linear polymerization shrinkage associated with the various cure modes during and post light polymerization up to 60 minutes. Five specimens were made for each cure mode. Data was analyzed using one-way ANOVA and Scheffe's post-hoc test at significance level 0.05. Post-gel shrinkage associated with PDI was significantly lower than with PDII, SS and PCI immediately post light-polymerization. At one-minute post light polymerization, PDI had significantly lower shrinkage compared to PDII and SS. Significant differences in shrinkage were observed between PDI and SS only at 10, 30 and 60 minutes. At all time intervals, no significance in post-gel shrinkage was observed between the control and all-pulse activation/soft-start polymerization regimens.

REP1, a basic helix-loop-helix protein, is required for a branch pathway of phytochrome A signaling in arabidopsis.

Phytochromes are primary photoreceptors mediating diverse responses ranging from induction of germination to floral induction in higher plants. We have isolated novel recessive rep1 (reduced phytochrome signaling 1) mutants, which exhibit a long-hypocotyl phenotype only under far-red light but not under red light. Physiological characterization showed that rep1 mutations greatly reduced a subset of phytochrome A-regulated responses, including the inhibition of hypocotyl elongation, cotyledon expansion, modulation of gravitropic growth of hypocotyl, and induction of the CAB (encoding chlorophyll a/b binding protein) gene, without affecting the accumulation of anthocyanin, far-red-preconditioned blocking of greening, induction of germination, and induction of CHS (encoding chalcone synthase) and FNR (encoding ferredoxin-NADP(+) oxidoreductase) genes. These results suggest that REP1 is a positive signaling component, functioning in a branch of the phytochrome A signaling pathway. Molecular cloning and characterization of the REP1 gene revealed that it encodes a light-inducible, putative transcription factor containing the basic helix-loop-helix motif.

Phytochrome signalling is mediated through nucleoside diphosphate kinase 2.

Because plants are sessile, they have developed intricate strategies to adapt to changing environmental variables, including light. Their growth and development, from germination to flowering, is critically influenced by light, particularly at red (660 nm) and far-red (730 nm) wavelengths. Higher plants perceive red and far-red light by means of specific light sensors called phytochromes(A-E). However, very little is known about how light signals are transduced to elicit responses in plants. Here we report that nucleoside diphosphate kinase 2 (NDPK2) is an upstream component in the phytochrome signalling pathway in the plant Arabidopsis thaliana. In animal and human cells, NDPK acts as a tumour suppressor. We show that recombinant NDPK2 in Arabidopsis preferentially binds to the red-light-activated form of phytochrome in vitro and that this interaction increases the activity of recombinant NDPK2. Furthermore, a mutant lacking NDPK2 showed a partial defect in responses to both red and farred light, including cotyledon opening and greening. These results indicate that NDPK2 is a positive signalling component of the phytochrome-mediated light-signal-transduction pathway in Arabidopsis.

Control of circadian rhythms and photoperiodic flowering by the Arabidopsis GIGANTEA gene.

Photoperiodic responses in plants include flowering that is day-length-dependent. Mutations in the Arabidopsis thaliana GIGANTEA (GI) gene cause photoperiod-insensitive flowering and alteration of circadian rhythms. The GI gene encodes a protein containing six putative transmembrane domains. Circadian expression patterns of the GI gene and the clock-associated genes, LHY and CCA1, are altered in gi mutants, showing that GI is required for maintaining circadian amplitude and appropriate period length of these genes. The gi-1 mutation also affects light signaling to the clock, which suggests that GI participates in a feedback loop of the plant circadian system.

Photomorphogenic development of the Arabidopsis shy2-1D mutation and its interaction with phytochromes in darkness.

We previously reported a photomorphogenic mutation of Arabidopsis thaliana, shy2-1D, as a dominant suppressor of a hy2 mutation. Here, we report that shy2-1D confers various photo-responsive phenotypes in darkness and the dark phenotypes of the mutant are affected by phytochrome deficiency. Dark-grown seedlings of the mutant developed several photomorphogenic characteristics such as short hypocotyls, cotyledon expansion and opening, and partial differentiation of plastids. When grown further in darkness, the mutant plant underwent most of the developmental stages of a light-grown wild-type plant, including development of foliar leaves, an inflorescence stem with cauline leaves, and floral organs. In addition, two light-inducible genes, the nuclear-encoded CAB and the plastid-encoded PSBA genes, were highly expressed in the dark-grown mutant seedlings. Furthermore, reduced gravitropism, a phytochrome-modulated response, was observed in the mutant hypocotyl in darkness. Thus, shy2-1D is one of the most pleiotropic photomorphogenic mutations identified so far. The results indicate that SHY2 may be a key component regulating photomorphogenesis in Arabidopsis. Surprisingly, double mutants of the shy2-1D mutant with the phytochrome-deficient mutants hy2, hy3(phyB-1) and fre1-1(phyA-201) showed reduced photomorphogenic response in darkness with a longer hypocotyl, a longer inflorescence stem, and a lower level expression of the CAB gene than the shy2-1D single mutant. These results showed that phytochromes function in darkness in the shy2-1D mutant background. The implications of these results are discussed.