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1.
Saudi J Biol Sci ; 31(2): 103919, 2024 Feb.
Article in English | MEDLINE | ID: mdl-38223132

ABSTRACT

Mosquito-borne diseases especially, dengue is gaining currency nowadays in Pakistan. As there is no approved dengue vaccine available worldwide, prevention and control of vector is the only solution amid prevailing circumstances. The present study is a maiden attempt to screen indoor and outdoor breeding containers for the presence of Aedes (Ae.) aegypti larvae from selected study districts of Punjab, Pakistan i.e., Dera Ghazi Khan (DG Khan), Chakwal, and Faisalabad. A total of 384 houses from each study districts were surveyed for a calendar year. Mosquito larvae were collected, preserved, and identified using standard taxonomic keys. House Index (HI), Container Index (CI), and Breteau Index (BI) were estimated. Chi-square analysis was applied to calculate the association between Ae. aegypti larvae and breeding containers. Chakwal was identified with the highest values of Stegomyia indices (HI = 46.61 %, BI = 91.67 %, and CI = 15.28 %) followed by Faisalabad (HI = 34.11 %, BI = 68.75 % and, CI = 13.04 %) and DG Khan (HI = 28.39 %, BI = 68.23 % and, CI = 11.29 %). Earthen jars, tree holes, and water tanks were found significantly (p < 0.05) associated with the abundance of larvae irrespective of the geographical location. However, flower tubs and plastic buckets were found significantly (p < 0.05) associated in Faisalabad and Chakwal while, tyres and plastic bottles were found associated (p < 0.05) with the abundance of Ae. aegypti larvae in Faisalabad and DG Khan. These findings will help the stakeholders to devise appropriate preventive measures in combating the risk of dengue transmission.

2.
Front Vet Sci ; 9: 1005079, 2022.
Article in English | MEDLINE | ID: mdl-36118345

ABSTRACT

In spite of the significant importance of the donkeys (Equus ascinus) as draft animal in resource-poor countries like Pakistan, they are equines not receiving the appropriate care. They face challenges including injuries, diseases, lack of basic environment and mismanagement by their owners. The present study aims to provide a brief update on the current status of management of healthcare and the welfare of domestic donkeys using participatory epidemiological tools. These tools can help to provide better strategies for improving their productivity and inclusion in human society. This study was mainly focused only on donkeys and horses, mules and ponies were excluded from the study. We carried out a systematic review of the relevant available published literature and shortlisted 50 articles reporting on the different health related characteristics of donkeys. A comprehensive questionnaire was completed by 191 donkey owners, including nine farriers (all men, average age = 38.24 ± 12.43) over a time span from October 2021 to March 2022. Multivariate Odds Ratios (MORs) and 95% confidence intervals were used to assess the predictions of health management and welfare measures for the surveyed donkeys. The most common observed medical problems in donkey health were hyperlipaemia (28.06%), lameness (16.33%) and dental (20.41%) problems. One-third (34.31%) of the donkeys were underweight. The dull donkey with poor appetite needs a clinical emergency owing to a high risk of developing hyperlipemia, which may be life-threatening. These findings are quite useful for the improvement of healthcare management and the welfare of donkeys.

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