ABSTRACT
Malignant melanoma is a cancer that arises from melanocyte cells in a complex but well-studied process, and which can only be successfully treated prior to metastasis as it is highly resistant to conventional therapies. A number of recent reports have indicated that members of the HOX family of homeodomain-containing transcription factors are deregulated in melanoma, and may actually be required to maintain proliferation. In this report, we describe the use of a novel, cell-permeable antagonist of the interaction between HOX proteins and PBX, a second homeodomain-containing transcription factor that modifies HOX activity. This antagonist can block the growth of murine B16 cells and trigger apoptosis both in vitro and in vivo when administered to mice with flank tumors.
Subject(s)
Antineoplastic Agents/pharmacology , Apoptosis/physiology , Homeodomain Proteins/antagonists & inhibitors , Melanoma/metabolism , Peptides/pharmacology , Transcription Factors/antagonists & inhibitors , Animals , Cell Line, Tumor , Cell Proliferation/drug effects , Genes, Homeobox , Homeodomain Proteins/chemistry , Humans , Intercellular Signaling Peptides and Proteins , Mice , Peptides/chemical synthesis , Reverse Transcriptase Polymerase Chain Reaction , Transcription Factors/chemistryABSTRACT
RARA becomes an acute promyelocytic leukemia (APL) oncogene by fusion with any of five translocation partners. Unlike RARalpha, the fusion proteins homodimerize, which may be central to oncogenic activation. This model was tested by replacing PML with dimerization domains from p50NFkappaB (p50-RARalpha) or the rapamycin-sensitive dimerizing peptide of FKBP12 (F3-RARalpha). The X-RARalpha fusions recapitulated in vitro activities of PML-RARalpha. For F3-RARalpha, these properties were rapamycin sensitive. Although in vivo the artificial fusions alone are poor initiators of leukemia, p50-RARalpha readily cooperates with an activated mutant CDw131 to induce APL-like disease. These results demonstrate that the dimerization interface of RARalpha fusion partners is a critical element in APL pathogenesis while pointing to other features of PML for enhancing penetrance and progression.
Subject(s)
Leukemia, Promyelocytic, Acute/metabolism , Leukemia, Promyelocytic, Acute/pathology , Receptors, Retinoic Acid/chemistry , Receptors, Retinoic Acid/metabolism , Animals , Bone Marrow/pathology , Carcinogens/metabolism , Cell Line , DNA/metabolism , DNA-Binding Proteins/metabolism , Dimerization , Down-Regulation/genetics , Mice , Mice, Transgenic , Mutation/genetics , Myeloid Cells/metabolism , Myeloid Cells/pathology , Neoplasm Proteins/metabolism , Oncogene Proteins, Fusion/metabolism , Protein Binding , Protein Structure, Quaternary , Receptors, Cytokine/metabolism , Receptors, Retinoic Acid/genetics , Recombinant Fusion Proteins/chemistry , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/metabolism , Repressor Proteins/chemistry , Repressor Proteins/genetics , Repressor Proteins/metabolism , Retinoic Acid Receptor alpha , Retinoid X Receptors/metabolism , Transcription, Genetic/geneticsABSTRACT
HOXA9 is a transcription factor with a central role in both haemopoiesis and leukaemia, and has been extensively studied over the past decade. Most notably, high levels of HOXA9 expression in haemopoietic cells is a characteristic feature of acute myeloid leukaemia (AML), and indeed may be sufficient in its own right to cause this disease. Here, we show that HOXA9 expression changes dramatically with age, whereby a uniformly low level of expression during early adulthood is replaced by a frequently very high expression in adults over sixty.
Subject(s)
Hematopoietic System/metabolism , Homeodomain Proteins/blood , Adolescent , Adult , Age Distribution , Aged , Aged, 80 and over , Female , Humans , Male , Middle Aged , Up-RegulationABSTRACT
HOXB4 is a homeodomain-containing transcription factor with diverse roles in embryonic development and the regulation of adult stem cells. Intriguingly, this gene can act in opposite ways when expressed by different cells, promoting the proliferation of stem cells whilst activating the apoptotic pathway in some embryonic structures. This review considers the basis for these differences in terms of the molecular biology of HOXB4 and the cells that express it.
Subject(s)
Homeodomain Proteins/physiology , Transcription Factors/physiology , Animals , Apoptosis/physiology , Cell Proliferation , Gene Expression Regulation, Developmental , Homeodomain Proteins/genetics , Stem Cells/physiology , Transcription Factors/geneticsABSTRACT
Development of the vertebrate eye is dependent on multiple tissue interactions and distinct sets of transcription factors and signalling molecules. The latter are thought to be FGF8 and BMP4, proteins secreted from the neural plate to induce lens formation in the overlying ectoderm. Here we show that the Pbx transcription factor plays a crucial role in this process, as it is required for FGF8 expression in the neural plate and is thus part of the complex hierarchy of genes involved in lens induction.
Subject(s)
Bone Morphogenetic Proteins/physiology , Fibroblast Growth Factors/physiology , Gene Expression Regulation, Developmental , Lens, Crystalline/embryology , Neurons/metabolism , Peptides/physiology , Animals , Base Sequence , Blotting, Western , Bone Morphogenetic Protein 4 , Bone Morphogenetic Proteins/metabolism , DNA Primers/chemistry , Ectoderm/metabolism , Fibroblast Growth Factor 8 , Genetic Vectors , Homeodomain Proteins/biosynthesis , Homeodomain Proteins/genetics , In Situ Hybridization , Models, Biological , Molecular Sequence Data , Neural Crest/metabolism , Peptides/metabolism , Phenotype , Polymerase Chain Reaction , Protein Binding , RNA/metabolism , Signal Transduction , Time Factors , Transcription, Genetic , Xenopus , Xenopus ProteinsABSTRACT
Acute promyelocytic leukemia (APL) is characterized by the PML-RARA fusion gene. To identify genetic changes that cooperate with PML-RARA, we performed spectral karyotyping analysis of myeloid leukemias from transgenic PML-RARA mice and from mice coexpressing PML-RARA and BCL2, IL3, activated IL3R, or activated FLT3. A cooperating mutation that enhanced survival (BCL2) was not sufficient to complete transformation and was associated with multiple numeric abnormalities, whereas cooperating mutations that deregulated growth and enhanced survival were associated with normal karyotypes (IL3) or simple karyotypic changes (IL3R, FLT3). Recurring abnormalities included trisomy 15 (49%), trisomy 8 (46%), and -X/-Y (54%). The most common secondary abnormality in human APL is +8 or partial trisomy of 8q24, syntenic to mouse 15. These murine leukemias have a defined spectrum of changes that recapitulates, in part, the cytogenetic abnormalities found in human APL. Our results demonstrate that different cooperating events may generate leukemia via different pathways.
Subject(s)
Chromosome Aberrations , Leukemia, Promyelocytic, Acute/genetics , Leukemia/genetics , Neoplasm Proteins/genetics , Oncogene Proteins, Fusion/genetics , Animals , Cell Division/genetics , Cell Survival/genetics , Cell Transformation, Neoplastic/genetics , Cell Transformation, Neoplastic/pathology , Karyotyping , Leukemia, Promyelocytic, Acute/etiology , Leukemia, Promyelocytic, Acute/pathology , Mice , Mice, TransgenicABSTRACT
The PML-RAR alpha fusion protein is central to the pathogenesis of acute promyelocytic leukemia (APL). Expression of this protein in transgenic mice initiates myeloid leukemias with features of human APL, but only after a long latency (8.5 months in MRP8 PML-RARA mice). Thus, additional changes contribute to leukemic transformation. Activating mutations of the FLT3 receptor tyrosine kinase are common in human acute myeloid leukemias and are frequent in human APL. To assess how activating mutations of FLT3 contribute to APL pathogenesis and impact therapy, we used retroviral transduction to introduce an activated allele of FLT3 into control and MRP8 PML-RARA transgenic bone marrow. Activated FLT3 cooperated with PML-RAR alpha to induce leukemias in 62 to 299 days (median latency, 105 days). In contrast to the leukemias that arose spontaneously in MRP8 PML-RARA mice, the activated FLT3/PML-RAR alpha leukemias were characterized by leukocytosis, similar to human APL with FLT3 mutations. Cytogenetic analysis revealed clonal karyotypic abnormalities, which may contribute to pathogenesis or progression. SU11657, a selective, oral, multitargeted tyrosine kinase inhibitor that targets FLT3, cooperated with all-trans retinoic acid to rapidly cause regression of leukemia. Our results suggest that the acquisition of FLT3 mutations by cells with a pre-existing t(15;17) is a frequent pathway to the development of APL. Our findings also indicate that APL patients with FLT3 mutations may benefit from combination therapy with all-trans retinoic acid plus an FLT3 inhibitor.
