[WEAK COMBINED MAGNETIC FIELDS ADJUSTED TO THE PARAMETRIC RESONANCE FOR Ca2+ INTENSIFY DYSTROPHIN SYNTHESIS IN MDX MICE SKELETAL MUSCLES AFTER CELL THERAPY].

The mdx mice are an X-linked myopathic mutants, an animal model for human Duchenne muscular dystrophy (DMD). Mdx mice muscles are characterized by high level of striated muscle fibers (SMF) death followed by regeneration. As a result most SMFs of mdx mice have centrally located nuclei. The possibility of using stem cells therapy for the correction of DMD is actively being studied. One of the approaches to the usage of bone marrow stem cells for cellular therapy of DMD is the replacement of bone marrow after irradiation by X-rays. This method however does not give significant increase of dystrophin synthesis in mdx mice muscles fibers. We have tried to affect the mice after bone marrow transplantation by weak combined magnetic fields adjusted to the parametric resonance for Ca2+(Ca(2+)-MF) based on the data that the weak combined magnetic fields influence on tissues regeneration. We observed a significant increase in the proportion of dystrophin-positive SMFs in group of mdx mice radiation chimera 5 Gy and 3 Gy which was additionally exposed in Ca(2+)-MF in comparison with the control mdx mice and the group of mdx mice radiation chimera 5 Gy and 3 Gy which was kept in terrestrial magnetic field 2 months after chimera preparation--up to 15.8 and 18.3%, respectively. Also, there was an accumulation of SMFs without central nuclei. These data indicate a significanly increased efficacy of cell therapy in the case of additional exposition in Ca(2+)-MF. Thus, the efficiency of bone marrow transplantation mdx mice after both in doses 3 and 5 Gy was considerably enhanced by additional exposition to Ca(2+)-MF. Apparently, such magnetic field can intensify functioning of donor's nuclei which had been incorporated into muscle fibers.

[Gypsy moth Lymantria dispar L. in the South Urals: Patterns in population dynamics and modelling].

The analysis is conducted on population dynamics of gypsy moth from different habitats of the South Urals. The pattern of cyclic changes in population density is examined, the assessment of temporal conjugation in time series of gypsy moth population dynamics from separate habitats of the South Urals is carried out, the relationships between population density and weather conditions are studied. Based on the results obtained, a statistical model of gypsy moth population dynamics in the South Urals is designed, and estimations are given of regulatory and modifying factors effects on the population dynamics.

[The concept of the development of the otorhinolaryngological service in Moscow under conditions of modernization of the healthcare system and single-channel funding].

This paper highlights the complex of organizational and socio-economic measures designed to modify the healthcare system in the city of Moscow, improve the quality of medical aid, and ensure its availability for the urban population. The proposed methods were developed based on the results of analysis of the data obtained in the course of checking the work of the otorhinolaryngological services of the city, analysis of effectiveness of using the hospital stock in the therapeutic and prophylactic facilities of Moscow.

Idiopathic scoliosis, growth zones, magnetic therapy.

BACKGROUND: The study has been performed to investigate the influence of pulsed magnetic field on the bone growth plates to get new grounds of magneto therapy in AIS treatment. MATERIALS AND METHODS: Were used methods of "strong" and "weak" pulsed magnetic fields influence. RESULTS: Application of pulsed magnetic field causes an authentic inhibition of chondrocytes' active proliferation processes, decreases the index of labeled nuclei, indicating the suppression of DNA synthesis, takes place an increase in the unit weight of the more "mature" differentiated chondrocytes. The final result of these effects is the accelerated synostosis of bones' growth plates. CONCLUSION: Regardless of the reasons that cause growth infringements, the operating organ in the chain is the body's growth plate. Therefore, the appliance of magnetic fields in AIS treatment can be considered as a perspective one concerning growth plates' functional activity local management. To our point of view, the potential of magneto therapy methods in child's orthopedic treatment is significantly higher compared with modern practice.

[Theoretical analysis of recompression-based therapies of decompression illness].

Theoretical analysis is concerned with the benefits of oxygen, air and nitrogen-helium-oxygen recompression schedules used to treat decompression illness in divers. Mathematical modeling of tissue bubbles dynamics during diving shows that one-hour oxygen recompression to 200 kPa does not diminish essentially the size of bubble enclosed in a layer that reduces tenfold the intensity of gas diffusion from bubbles. However, these bubbles dissolve fully in all the body tissues equally after 2-hr. air compression to 800 kPa and ensuing 2-d decompression by the Russian navy tables, and 1.5-hr. N-He-O2 compression to this pressure followed by 5-day decompression. The overriding advantage of the gas mixture recompression is that it obviates the narcotic action of nitrogen at the peak of chamber pressure and does not create dangerous tissue supersaturation and conditions for emergence of large bubbles at the end of decompression.

[Experimental study of magnetic field influence on transitory instability of display image]. / Eksperimental'noe issledovanie vliianiia vneshnego magnitnogo polia na vremennuiu nestabil'nost' izobrazheniia displeia.

The article presents results of experimental study that determined threshold level of external magnetic field causing no flashing effect and spectral distribution of image instability, if general and local influence of magnetic field on display.

[The synthesis of phosphates of long-chain omega-hydroxyalkyl esters of 11-deoxyprostaglandin E1]. / Sintez fosfatov dlinnotsepochechnykh omega-gidroksialkilovykh éfirov 11-dezoksiprostaglandina E1.

Di(p-methylbenzyl) phosphates of omega-hydroxyalkyl esters of 11-deoxyprostaglandin E1 were synthesized from disubstituted 1,10-decane and 1,22-docosane derivatives for studying permeability of bilayer membranes. The English version of the paper.

[The possibilities of arifon in the combined therapy of unstable stenocardia in persons with arterial hypertension]. / Izuchenie vozmozhnostei arifona v kompleksnoi terapii nestabil'noi stenokardii u lits s arterial'noi gipertoniei.

The authors studied the effect of arifon on vascular-platelet hemostasis in patients with unstable angina pectoris and hypertension. It was established that arifon addition to standard treatment leads to a decrease of platelet aggregation. A degree of spontaneous aggregation under arifon administration reduced to 30.9%, the rate of spontaneous aggregation decreased to 47.6% versus 13.3 and 24.6%, respectively, in arifon-untreated patients. Arifon promoted reduction of ADP-induced platelet aggregation to 26.9% (without arifon 6.5%); p < 0.01.

[Effect of a 50 Hz magnetic field on rat behavior depends on the direct current component]. / Vliianie magnitnogo polia chastotoi 50 Gts na povedenie krys zavisit ot znacheniia postoiannogo magnitnogo polia.

The influence of extremely low frequency magnetic field on rats behavior (working memory, learning, social activity) was examined. Rats were exposed to magnetic fields at three different conditions: exposure 1-50 Hz, 46 mT; DC 65 mTl; exposure II-50 Hz, 46 mTl; DC 14-17 mTl; Exposure III-sham. Field related changes were observed after exposure I: the increase of error rate in Y-maze performance, shorter adaptation time in instrumental performance, the decrease of aggression and sociability, enhancement of the defence reactions. Data obtained show that influence of 50-Hz magnetic field depends on the level of DC field. The combination presumably considered as resonant to Ca++ was more effective.

[Total synthesis and properties of prostaglandins. XXXVIII. Synthesis of 11-deoxyprostaglandin-E1 derivatives with amino acids and amines]. / Total'nyi sintez i svoistva prostaglandinov. XXXVIII. Sintez proizvodnykh 11-dezoksiprostaglandina-D1 s aminokislotami i aminami.

In order to study the physiological functions of 11-deoxyprostaglandin E1-alpha, a series of its amide derivatives with amino acids and some amines were synthesized using mixed anhydride technique. The myotropic properties of newly synthesized compounds were investigated.

[Total synthesis and properties of prostaglandins. XXXI. Synthesis of pyranylated and glycosidated omega-hydroxyalkyl ethers of 11- deoxyprostaglandin E1]. / Total'nyi sintez i svoistva prostaglandinov. XXXI. Sintez piranilirvannykh i glikozidirovannykh omega-gidroksialkilovykh éfirov 11-dezoksiprostaglandina E1.

Four methods of the synthesis of model glycosides with 11-deoxyprostaglandin E1 and a connecting polymethylene chain as the aglycone are compared. Interaction of potassium salt of prostaglandin PG with omega-iodoalkylglycosides is the most promising approach.

[Total synthesis and properties of prostaglandins. XXIV. Synthesis of glycerides with prostaglandin acyl residues]. / Total'nyi sintez i svoistva prostaglandinov. XXIV. Sintez glitseridov s prostaglandinovym atsil'nym ostatkom.

Synthesis of a number of model triglycerides and lysoglycerides bearing the acyl moieties of 11-deoxyprostaglandin E1, and palmitic acid residues has been carried out.

[Complete synthesis and properties of prostaglandins. X. Hydroxyl-containing esters of 11-deoxyprostaglandin E1]. / Polnyi sintez i svoistva prostaglandinov. X. Gidroksilsoderzhashchieéfiry 11-dezoksiprostaglandina E1.

Hydroxyl-containing 11-deoxyprostaglandin E1 esters were synthesised by using the methods of mixed anhydrides and nucleophilic displacement.

[Pharmacokinetics of rifampicin administered intravenously to pulmonary tuberculosis patients]. / Farmakokinetika rifampitsina pri vnutrivennom vvedenii u bol'nykh tuberkulezom legkikh.

Pharmacokinetics of rifampicin studied in 15 patients after its intravenous administration in a dose of 10 mg/kg was described by a two-compartment model. The drug levels in serum and urine were determined with a chemical method. At the moment of the infusion discontinuation the maximum drug levels in serum averaged to 14.05 micrograms/ml. The mean values of the total clearance, distribution of the kinetic volume and half-lives were 93.2 ml/(h X kg), 1016.1 ml/kg and 8.1 h respectively. Cumulative excretion of the total rifampicin within 24 hours amounted to 19.4 per cent of the administered dose, the proportions of the main metabolite (25-O-desacetyl rifampicin) and intact rifampicin being equal to 29 and 71 per cent respectively. The renal and nonrenal clearance of rifampicin amounted to 14.2 and 79 ml/(h X kg) respectively.

Analysis of difference spectra of protonated DNA: determination of degree of protonation of nitrogen bases and the fractions of disordered nucleotide pairs.

The titration curves of nitrogen bases and fractions of disordered nucleotide pairs are obtained during DNA protonation. It is shown that purine bases are the first sites of the DNA double helix protonation. The cytosine protonation is due to proton-induced conformational transition within GC pairs with the sequence proton transfer from (N-7) of guanine to (N-3) of cytosine. Within DNA with unwound regions the bases are protonated in the following order: cytosine, adenine, guanine. It is shown that GC pairs are the primary centres in which the unwinding of protonated DNAs occurs.

[Effect of inhibitors of microsomal hydroxylation on endogenous respiration of Torulopsis candida cells]. / Vliianie ingibitorov mikrosomal'nogo gidroksilirovaniia na éndogennoe dykhanie kletok Torulopsis candida.

The effect of inhibitors of microsomal cytochrome P-450 on endogenous respiration and the redox state of components in the electron transport chain was studied in the cells of Torulopsis candida. SKP-525 A and methyrapone in the presence of preliminarily added cyanide (or antimycin A) almost completely inhibited endogenous respiration of the cells irrespective of the growth phase of the culture. The effect of these inhibitors on endogenous respiration and the reduction state of pyridine nucleotides, flavoproteins and cytochromes b, c and a + a3, at all of the studied growth phases, was similar to that of hydroxamic acids, the inhibitors of the mitochondrial alternative oxidase system. Contrary to their action on endogenous respiration of the cells at the exponential phase, both benzhydroxamic acid (BHA) and the inhibitors of microsomal hydroxylation, in the presence of rotenone, suppressed endogenous respiration of the cells taken at the stationary phase. Therefore, the interaction of the electron transport chains changed in the process of the cultural growth. Addition of BHA to the medium inhibited growth of the culture on glucose and biosynthesis of cytochrome P-450 in these cells. The culture did not grow on hexadecane in the presence of BHA. Apparently, hydroxamic acids can affect the operation of the microsomal monooxigenase system containing cytochrome P-450.