[Problems in the diagnosis and surgical treatment in an osseous tuberculosis hospital].

The diagnosed patients treated at an osseous tuberculosis hospital are analyzed. In the reporting period, the diagnosis of osseous tuberculosis has not been verified. Late detection of osseous tuberculosis, as its prehospital hyperdiagnosis is one of the main reasons of a grave condition in this group of patients. The paper characterizes plastic reparative versus palliative decompressive operations for tuberculous spondylitis with spinal-cord abnormalities and shows that radical surgery has a higher efficiency (89%) in the treatment of an inflammatory process and in the regression of neurological complications. In generalized forms of osteoarticular lesions if there is an indication for surgical treatment, long-term drug therapy for tuberculosis of the lung and other organs is not warranted.

[Slow photoinduced changes in reaction centers of Rhodobacter sphaeroides R-26 by holographic interferometry]. / Issledovanie medlennykh fotoindutsirovannykh izmenenii v reaktsionnykh tsentrakh Rhodobacter sphaeroides R-26 metodom golograficheskoi interferometrii.

Using the on-line holographic interferometry, changes in the refraction index of illuminated solutions containing the Rhodobacter sphaeroides R-26 reaction centers isolated from membranes by detergents of ionic and nonionic nature were registered. Factors affecting the refraction index of these solutions were analyzed. It was shown that the photoinduced changes in this parameter are due to structural changes in the molecular complex of reaction centers. The results of measurements of the kinetics of absorption spectra at lambda = 865 nm for two detergent types are presented, which coincide qualitatively with the results of holographic measurements. The change in the volume of the molecular complex of reaction centers was estimated to be 0.1 divided by 1%.

Correlation of amino acid residues in the M1 and M2 proteins of influenza virus with high yielding properties.

The ability of influenza A viruses to replicate to high titer in the allantoic sac of the chicken embryo has been mapped to the matrix protein gene (RNA 7). Because influenza A/WSN/33 (H1N1) virus grows poorly in this host but contains a matrix protein gene with a sequence similar to sequences from viruses that grow well in eggs, we derived a single gene reassortant containing only the M gene from A/WSN/33 (H1N1) in a background of the other 7 RNA segments from A/Philippines/2/82 (H3N2) (a low yielding virus, hy-). This reassortant replicated 10 times better than the A/WSN parent itself, indicating that the high yielding (hy+) phenotype of the A/WSN/33 M gene may be suppressed by one of the other genes of A/WSN/33. Comparison of M gene sequences between hy+ (including A/WSN/33) and hy- strains allowed us to correlate specific amino acid positions in M1 and M2 proteins with the growth properties of influenza viruses.