[Effect of biologically active food additives containing autolysate of baker's yeast and spirulina on intestinal permeability in an experiment]. / Vliianie biologicheski aktivnykh dobavok k pishche, soderzhashchikh avtolizat pekarskikh drozhzhei i spirulinu, na pronitsaemost' kishechnika v éksperimente.

Influence of bioactive food supplements (BFA) intake on intestinal barrier permeability to macromolecules of polyethylene glycol 4000 was studied in rats with intestinal anaphylaxis and after external gamma-irradiation. BFA studied included autolysed baker's yeast ("Vitasil") and edible algae Spirulina platensis. Intake of complex additive Vitasil + Spirulina resulted in significant diminution of permeability before irradiation and its partial normalization (24% decrease) after irradiation. Spirulina additive intake led to practically complete normalization of permeability (1.84 times decrease) in anaphylactic rats. It is concluded that Spirulina and Vitasil are promising BFA for organism general resistance elevation.

[The effect of fermented hydrolyzed whey enriched with lactates on the morphological structure of the internal organs in laboratory animals]. / Vliianie fermentativno-gidrolizovannoi molochnoi syvorotki, obogashchennoi laktatami, na morfologicheskuiu strukturu vnutrennikh organov laboratornykh zhivotnykh.

Results of pathomorphological investigation of rat organs fed during 1 and 3 months the diet containing 8% of protein as milk whey protein partly hydrolysed by enzymes and enriched by lactates (SGOL-1) are presented. The conducted investigation have not revealed of pathological effect of a product SGOL-1 on morphological structures of investigated internal bodies and tissues of the laboratory animals. A increase of body mass of animals and contents of RNAS in cells of practically all bodies and tissues of the animals were also marked.

[Biomedical aspects of the use of milk whey in nutrition (review)]. / Mediko-biologicheskie aspekty ispol'zovaniia molochnoi syvorotki v pitanii (obzor).

The present review of biotechnological research-works is connected with the use of milk whey in nutrition. The review deals with the use of milk whey in nutrition. The review deals with those aspects of the problem which prevent the widest use of milk whey possible in this field. A detailed review of technological methods is given, as far as their role in increasing the nutritional and biological value of milk wheys is concerned. The review also contains new data about modern ways of milk whey processing and a brief evaluation of the final products.

[The hygienic evaluation of modified Alaska pollack protein based on pathomorphological research]. / Gigienicheskaia otsenka modifitsirovannogo belka mintaia na osnove patomorfologicheskikh issledovanii.

It has been established that daily inclusion of modified Alaska pollack protein into the rat ration as a 10% substitute of casein does not induce any changes in the body. A long term (during 12 months) feeding of rats with the rations containing 50 and 100% of Alaska pollack protein, as casein substitute, results in significant changes in the histological structure of the kidneys and liver. The data of electron microscopy enabled the authors to make a conclusion on the presence of a toxic factor in the protein product studied.

[Features of the digestive function of the intestines during intake of nontraditional protein]. / Osobennosti pishchevaritel'noi funktsii kishechnika pri potreblenii netraditsionnogobelka.

Hydrolysis of modified walleye pollock protein under the action of pepsin pancreatic and intestinal enzymes was studied under conditions in vitro and in vivo. It was found that the degree of dissolution of bonds is decreased in the experiments in vitro by 18.05-24.87%, in vivo by 34.2%, as compared to the experiments where casein was used. At the same time hydrolysis of oligo- and dipeptides realized in the intestine by amino- and dipeptidases is stimulated under the influence of the pollock protein by 16.07-53.7%. Therefore it is suggested that utilization of the protein studied depends on the capacity of peptidases to release dipeptides in separate acetylated or free dipeptides. Thus, for the sake of regulatory and adaptive changes of secretion of some digestive glands and especially enzymes, fixed on enterocyte brush edge, the necessary enzyme-substrate correspondence is provided, as a result of which hydrolysis of the pollock protein is achieved.

[Features of the absorption of modified fish proteins]. / Osobennosti vsasyvaniia modifitsirovannykh rybnykh belkov.

The characteristics of absorption of modified fish protein were studied in comparison with those non-modified. It has been established that protein acetylation process reduces the absorption intensity of lysine, sulfur-containing amino acids as well as amino acids with a ramified chain. At the same time the final stages of protein assimilation on the surface of the small intestine mucosa are attended by appearance of amino acids at the free state, except lysine which in the form of acetyllysine enters blood flow. Thus, further investigation of the character of acetylated protein metabolism in the body is necessary, as their practical use in food industry is promising.

[The role of amino acids in the regulation of gastric secretion]. / Uchastie aminokislot v reguliatsii zheludochnoi sekretsii.

The experiments conducted on dogs have demonstrated that amino acids entering blood increase the maximum response of gastric glands to pentagastrin 2-3-fold, to histamine by 20-25%. The test on rats has revealed varying concentrations of amino acids when they are passing through the liver: a half of certain amino acids (lysine, glycine, proline etc.) is retained in the liver, other (cystine) increase their concentration in the hepatic vein as compared to that in the portal vein, whereas amino acids with ramified carbon chain are insignificantly retained by the liver. The data obtained have evidenced an important role of amino acids in the third intestinal phase of gastric secretion during protein food intake and in the modulating of gastric secretion under conditions of mixed food intake.

[Dynamics of the uptake of free amino acids in the blood serum of the portal vein during the digestion of isolated krill protein]. / Dinamika postupleniia svobodnykh aminokislot v syvorotku krovi vorotnoi veny v protsesse perevarivaniia izolirovannogo belka krilia.

The rate of absorption of amino acids obtained from isolated krill protein administered to rat stomach was studied. Absorption of casein amino acids was determined for reference purposes. Rats subjected to 48-hour fasting were administered krill isolate and casein in the form of homogenates at a rate 540 mg protein per 100 g bw in 6 ml of distilled water. The concentration of free amino acids in the portal vein was measured after 30 minutes, 1, 3 and 6 hours. The content of amino acids was measured by ion-exchange chromatography with the use of an automatic analyzer of amino acids. The digestion of krill protein proceeds at a high rate. This ensures the maximal augmentation of the concentration of most amino acids in the blood of the portal vein 30 min after isolate administration to the stomach.

[Amino acid metabolism in liver tissue during the consumption of qualitatively different proteins]. / K voprosu o metabolizme aminokislot v tkani pecheni pri potreblenii kachestvenno razlichnykh belkov.

To evaluate changes in the liver ability to metabolize amino acids during intake of different proteins, the content of free amino acids was measured in the blood of the portal and liver veins of rats fed krill protein and casein. After 48 h of fasting the animals were given a suspension of krill protein and casein at a rate of 540 mg protein per 100 g bw. After 0.5, 1, 3 and 6 h the content of free amino acids was measured in the blood of the portal and liver veins by ion exchange chromatography with the use of an automatic amino acid analyzer. Administration of krill protein brought about a high enough (63%) absorption of amino acids by the liver during the 6-hour experiment. The degree of casein absorption was 31%. As compared to casein, intake of krill protein led to an earlier saturation of the body with amino acids having branched chains (P less than 0.05). The enzymatic systems of amino acid metabolism responded to the supply of the proteins under study in the same manner, however the rate of adaptation was different, amounting to 0.5 h for krill protein and to 3 h for casein. It is evident that the enzymatic systems involved in amino acid metabolism respond to the intake of krill protein to a larger extent than enzymes involved in metabolism of casein amino acids.

[Principles and methods of hygienic evaluation of new protein products]. / Printsipy i metody gigienicheskoi otsenki novykh belkovykh produktov.

Modern approaches to the study of the quality of new protein products are reviewed. It is suggested that the food value of the products (organoleptic properties, the composition of nutrients, biological value) and their safety for the animal body should be examined. A possible chronic toxic and sensitizing action of new protein products, their effects on reproductive function, and mutagenic and cancerogenic action were studied in animal experiments.