A biosynthesis activity of Streptomyces recifensis var. lyticus.

The structure of enzyme complex synthesized of soil streptomycete was investigated. Fractionation and purification of enzymes have allowed to identify a line of hydrolases: proteinases, amylases, DNA-ses, cellulases and also glycosidases and endopeptidases with lytic action. Their properties were studied. The isolated lytic endopeptidases had a wide substrate specificity and hydrolyzed casein and synthetic peptides at pH 7-9 and also a cell wall of bacteria at pH 5-6. The temperature optimum of 5 researched endopeptidases are in the range 45-60 degrees C. They were activated by ions Co2+, Fe2+, Cu2+ to a different degree. The universal activators of lytic endopeptidases were ions Cd2+ and Pb2+. They have raised lytic activity of enzymes 3-7 times. The obtained data can witness high adaptogenic ability of streptomycetes in relation to heavy metals. The possibility of enzyme preparation from Streptomyces recifensis use for growth stimulation of agricultural plants was shown also against the wheat, oats, maize, haricot beans, peas, barley.

[The development of intestinal dysbacteriosis in sexually mature rats after thymectomy]. / Razvitie disbakterioza kishechnika u polovozrelykh krys posle timéktomii.

Thymectomy in puberal rats resulted in the development of pronounced enteric dysbacteriosis, correlating with the degree of T- and B-lymphocyte deficiency in the lymphoid organs and the blood stream during the first 2 months. Microbiocenosis restores probably due to more intensive functioning of alternative regulating mechanisms. A simple and informative characteristic indicating the degree of dysbacteriosis developing in the intestine is proposed: the index of dysbacteriosis.

[Chemotherapeutic activity of lytic enzymes in experimental staphylococcal infection]. / Khimioterapevticheskaia aktivnost' liticheskikh fermentov pri éksperimental'noi stafilokokkovoi infektsii.

It was shown possible to use lytic enzymes from Streptomyces recifensis subsp. lyticus 2435 for chemotherapeutic purposes. After fractionation with ammonium sulfate or chromatographic purification on sephadex G-75, the enzymatic preparations were found to be complex and contain a number of proteinases and lytic endopeptidases. They were highly active in vitro in lysing thick staphylococcal suspensions. The local use of the preparations provided a favourable therapeutic effect on a model of experimental staphylococcal infection of albino mice and rats. It was evident from rapid arrest of the inflammatory process or earlier maturation and opening of abscesses and their cicatrization. The contribution of nonlytic proteinases to the process is not excluded.

[The role of nonsporulating anaerobic bacteria in the etiology of postabortion and postpartal endometritis]. / Rol' nesporoobrazuiushchikh anaérobnykh bakterii v étiologii posleabortnogo i poslerodovogo éndometritis.

As the result of the study carried out by the authors, a conclusion has been made on the leading role of polymicrobial aerobic-anaerobic complexes, capable of producing a synergic effect, in the etiology of endometritis, anaerobic microorganisms prevailing in their importance. In the etiology of endometritis the most important organisms are bacteroids and peptostreptococci among anaerobes, enterobacteria and group D streptococci among aerobes. Anaerobic bacteria causing endometritis are most sensitive to dalacin and lincomycin, least sensitive to benzylpenicillin, resistant to aminoglycosides. The amounts of aerobic and anaerobic microflora contaminating the uterine cavity correlate with the severity of endometritis.

[Etiological structure and antibiotic sensitivity of the causative agents of puerperal endometritis]. / Etiologicheskaia struktura i chuvstvitel'nost' k antibiotikam vozbuditelei poslerodovogo éndometrita.

The study revealed the dominating role of aerobic-anaerobic microbial associations and in particular the specific role of anaerobic gram positive cocci in development of puerperal endometritis. The data suggested that a definite level of the uterus cavity contamination with microbes, not lower than 10(4)-10(5) CFU/ml or a large number of bacterial associates, not less than 3 was necessary for endometritis development. It was confirmed that pathogenicity of anaerobes increased in the presence of aerobic bacteria. It is concluded that quantitative methods for detecting the main causative agents of endometritis are needed. A set of antibacterial drugs for rational antibacterial therapy of puerperal endometritis is recommended.

[Antibiotic sensitivity of the microflora isolated from the uterine cavity of patients with postabortion endometritis]. / Chuvstvitel'nost' k antibiotikam mikroflory, vydelennoi iz polosti matki bol'nykh posleabortnym éndometritom.

Examination of the uterus cavity microflora in 50 patients with endometritis post abortum revealed that the etiology of endometritis post abortum was polymicrobial and its development was mainly due to aerobic and anaerobic microbial associations. By the isolation frequency enterococci predominated among the aerobic organisms and bacteriods and fusobacteria predominated among the anaerobic organisms. All the pathogens isolated from the uterus cavity were characterized by polyresistance to antimicrobial drugs. The aerobic bacteria were often resistant to the antibiotics active against the anaerobic organisms which required the use of combined antibacterial therapy in some cases.